Báo cáo khoa học: Expression of the Drosophila melanogaster ATP synthase a subunit gene is regulated by a transcriptional element containing GAF and Adf-1 binding sites pptx

... this reason, ATP synthase and in particular the a- F1-ATPase and b-F1-ATPase catalytic subunits have been often used as markers for mitochondrial biogenesis [6,31,44,45]. The Drosophila a- F1-ATPase ... a- F1-ATPase GAF/ Adf-1 binding cassette has enhancer properties. (A) The basal promoter activity of b-F1-ATPase is greatly increased when the a- F1-ATPase GAF/...

Ngày tải lên: 07/03/2014, 16:20

... in Table 1. Plasmid pCJ31B contains the L. lactis pyrG gene, and was made from a PCR-product made with prim- ers pyrG1 1a (5¢-GTAGAAGCTAAAATCTGG-3¢ )and SLLH7 (5¢-TACAAAAGATTTTGGGC-3¢) cloned in the ... control on the CTP and dCTP concentrations and a negative control on the UTP concentration. Materials and methods Bacterial strains and plasmids The strains and pl...

Ngày tải lên: 16/03/2014, 16:20

... A portion of the lysate was separated by 10% SDS ⁄ PAGE and autoradiographed. A mock-programmed lysate was run in paral- lel. Molecular mass markers are shown at the left. An arrowhead indicates ... 5¢-GCCGAATTCCAACATGGC AGCACCAGAGACGGG-3¢;5¢-GCCTCTAGATCATCG CAGCGTGCTGGTCAG-3¢. The amplified products were purified, digested and subcloned into EcoRI and XbaI sites of pBlu...

Ngày tải lên: 16/03/2014, 18:20

... All the characteristics of the recombinant laccase are in agreement with those of the native laccase. This is the first report of the production of a white-rot laccase in A. niger. Keywords:laccase;Pycnoporus ... et al.[21].An aliquot of 15 lg o f total RNA was denatured a t 6 5 °Cina loading buffer mixture containing formamide and form- aldehyde [22] and loade...

Ngày tải lên: 17/03/2014, 11:20

... from xylem, and vice versa. Glx (glutamine and glutamate: five carbon amide and amino acid) and Asx (asparagine and aspartate: four carbon amide and amino acid) were found to be the main nitrogen ... in maize, and the regulation and function of each gene have been elucidated in part [2–4]. Ammonium and glutamine-amide group are also assimilated into asparagine by as...

Ngày tải lên: 18/02/2014, 18:20

... 5¢-CAGCTTGCTCACAAT CATCC-3¢ and 5¢-CTCATTCCAAGCCCGAGAAAC-3¢. The 4.5-kb product was purified with the GFX Ô PCR DNA and Gel Band Purification Kits GFX (Amersham Biosciences, Piscataway, NJ, USA) and ... amplification of al-2 using the primers 5¢-TCC AAGCTTCTATATGACAATAGCGCC-3¢ and 5¢-CCAG GATCCGTCTACTGCTCATACAAC-3¢, deduced from the A. Prado-Cabrero et al. Alteration of Fusa...

Ngày tải lên: 30/03/2014, 01:20

... Biovitrum AB. Journal compilation ª 2007 FEBS 2887 all of which are required for its activity [8,9]. In the mammalian genome, two isoforms of the a- subunit (a1 and a2 ) and the b -subunit (b1 and b2), and ... substitutions in the AMP -binding Bateman domains of c3. We demonstrate that AMP-activated protein kinase a2 b2c3 trimers are allosterically activated appro...

Ngày tải lên: 30/03/2014, 08:20

... AAG AAC CAA CAA GAT GAA RT-PCR for neuronal differentiation MAP2 reverse AAT CAA GGC AAG ACA TAG CGA RT-PCR for neuronal differentiation b-actin forward TGA CGG GGT CAC CCA ACT GTG CCC ATC TA ... piRNAs are not generated by Dicer, both sense and antisense strands of the piRNA hairpin are involved in formation of the piR- NA-interacting complex (piRC) and function in the trans...

Ngày tải lên: 18/02/2014, 17:20

... aa 60 aa 38 aa 16 aa 5828 bp 3262 bp 23 aa 60 aa 38 aa 16 aa 7629 bp 6815 bp 7470 bp 23 aa 60 aa 38 aa 16 aa 1959 bp 3453 bp 1222 bp 23 aa 60 aa 38 aa 16 aa 554 bp 2277 bp 4313 bp 23 aa 60 aa ... showing similarity to the zebrafish CRABPIa and the mammalian CRAB- PIs (Fig. 1A) . The structure and coding capacity of this gene was identical to that of the mammalian and A B...

Ngày tải lên: 16/03/2014, 22:20

... antibody against acetylated a- tubuline and visualized with Alexa 488. TauT (red) was marked with a primary antibody raised against the C-terminus of TauT and visualized with Alexa 568. The merged ... against the N-terminal part of TauT, and immunolocalization of the antibody raised against the C-terminal part of TauT was abolished by a specific blocking peptide...

Ngày tải lên: 30/03/2014, 15:20