Báo cáo khoa học: Expression of the Drosophila melanogaster ATP synthase a subunit gene is regulated by a transcriptional element containing GAF and Adf-1 binding sites pptx

... this reason, ATP synthase and inparticular the a- F1-ATPase and b-F1-ATPase catalyticsubunits have been often used as markers for mitochondrialbiogenesis [6,31,44,45]. The Drosophila a- F1-ATPase ... a- F1-ATPase GAF/ Adf-1 binding cassette has enhancer properties. (A) The basal promoter activity of b-F1-ATPase is greatly increased when the a- F1-ATPase GAF/ Adf-1 binding cassette is cloned upstream, ... 2004 Drosophila a- F1-ATPase gene expression (Eur. J. Biochem. 271) 4013 Expression of the Drosophila melanogaster ATP synthase a subunit gene is regulated by a transcriptional element containing...

Báo cáo khoa học: Expression of the pyrG gene determines the pool sizes of CTP and dCTP in Lactococcus lactis doc

... inTable 1. Plasmid pCJ31B contains the L. lactis pyrG gene, and was made from a PCR-product made with prim-ers pyrG1 1a (5¢-GTAGAAGCTAAAATCTGG-3¢ )and SLLH7 (5¢-TACAAAAGATTTTGGGC-3¢) cloned in the ... control on the CTP and dCTP concentrations and a negative control on the UTPconcentration.Materials and methodsBacterial strains and plasmids The strains and plasmids used in this study are listed ... the availability of purines and pyrimidines. The pyrimidine biosynthetic genes are regulated by the RNA -binding regulatory protein PyrR that regulates gene expression by an attenuation mechanism...

Báo cáo khoa học: Characterization of the Drosophila Methoprene -tolerant gene product Juvenile hormone binding and ligand-dependent gene regulation potx

... A portion of the lysate was separated by 10% SDS ⁄ PAGE and autoradiographed. A mock-programmed lysate was run in paral-lel. Molecular mass markers are shown at the left. An arrowheadindicates ... 5¢-GCCGAATTCCAACATGGCAGCACCAGAGACGGG-3¢;5¢-GCCTCTAGATCATCGCAGCGTGCTGGTCAG-3¢. The ampliﬁed products werepuriﬁed, digested and subcloned into EcoRI and XbaI sites of pBluescript II (Stratagene), and ... have led to the implication of another class of transcriptional regulator in JH signaling. Wilson and coworkers examined the resistance mechanism of Dro-sophila to the JHA, methoprene, and isolated...

Báo cáo khoa học: Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme pdf

... All the characteristics of the recombinantlaccase are in agreement with those of the native laccase. This is the ﬁrst report of the production of a white-rot laccase in A. niger.Keywords:laccase;Pycnoporus ... et al.[21].Analiquot of 15 lg o f total RNA was denatured a t 6 5 °Cinaloading buffer mixture containing formamide and form-aldehyde [22] and loaded on a 1% Tris/acetate/EDTAagarose gel containing ... su bstrate.Sd 194 kDa67 kDa43 kDa30 kDa20 kDaFig. 5. SDS/PAGE gel analysis of the pure laccase. Sd, molecular massstandards a nd lane 1, pure recombinant laccase stained withCoomassie...

Tài liệu Báo cáo khoa học: Implication of the glutamine synthetase ⁄glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves doc

... from xylem, and viceversa. Glx (glutamine and glutamate: ﬁve carbonamide and amino acid) and Asx (asparagine and aspartate: four carbon amide and amino acid) werefound to be the main nitrogen ... inmaize, and the regulation and function of each gene have been elucidated in part [2–4]. Ammonium and glutamine-amide group are also assimilated intoasparagine by asparagine synthetases (ASs; ... syn-thetase isoenzyme GSp1in maize (Zea mays). J PlantPhysiol 160, 601–605.5 Skopelitis DS, Paranychianakis NK, Paschalidis KA,Pilakonis ED, Dellis ID, Yakoumakis DI, Kouvarakis A, Papadakis AK,...

Báo cáo khoa học: Deviation of the neurosporaxanthin pathway towards b-carotene biosynthesis in Fusarium fujikuroi by a point mutation in the phytoene desaturase gene ppt

... 5¢-CAGCTTGCTCACAATCATCC-3¢ and 5¢-CTCATTCCAAGCCCGAGAAAC-3¢. The 4.5-kb product was puriﬁed with the GFXÔPCR DNA and Gel Band Puriﬁcation Kits GFX (Amersham Biosciences,Piscataway, NJ, USA) and ... ampliﬁcation of al-2 using the primers 5¢-TCCAAGCTTCTATATGACAATAGCGCC-3¢ and 5¢-CCAGGATCCGTCTACTGCTCATACAAC-3¢, deduced from the A. Prado-Cabrero et al. Alteration of Fusarium phytoene desaturaseFEBS ... Ss,NP344226), and the plant Arabidopsis thaliana (PDS3 At, Q07356). The comparison also includes the A. thaliana f-carotene desaturase(ZDS1 At, Q38893) and the human all-trans-retinol 13,14-reductase...

Báo cáo khoa học: Regulation of the muscle-speciﬁc AMP-activated protein kinase a2b2c3 complexes by AMP and implications of the mutations in the c3-subunit for the AMP dependence of the enzyme docx

... Biovitrum AB. Journal compilation ª 2007 FEBS 2887all of which are required for its activity [8,9]. In the mammalian genome, two isoforms of the a- subunit (a1 and a2 ) and the b -subunit (b1 and b2), and ... substitutions in the AMP -binding Bateman domains of c3.We demonstrate that AMP-activated protein kinase a2 b2c3 trimers areallosterically activated approximately three-fold by AMP with a half-maximal stimulation ... both the total activity and the AMP dependence (Fig. 1C). We measured the activity of the recombinant AMPK a2 b2c3 complexesover a range of AMP concentrations, and calculated the concentration of...

Tài liệu Báo cáo khoa học: Bioimaging of the unbalanced expression of microRNA9 and microRNA9* during the neuronal differentiation of P19 cells doc

... AAG AAC CAA CAA GAT GAA RT-PCR for neuronal differentiationMAP2 reverse AAT CAA GGC AAG ACA TAG CGA RT-PCR for neuronal differentiationb-actin forward TGA CGG GGT CAC CCA ACT GTG CCC ATC TA ... piRNAs are not generated by Dicer, both sense and antisense strands of the piRNA hairpin are involved in formation of the piR-NA-interacting complex (piRC) and function in the transcriptional gene ... white bar represents mature miR9 and the black bar maturemiR9*, and the line with a triangular head indicates the ratio(numerical values shown on the top of bar) of mature miR9 tomiR9*. Experiments...

Báo cáo khoa học: Retention of the duplicated cellular retinoic acid-binding protein 1 genes (crabp1a and crabp1b) in the zebraﬁsh genome by subfunctionalization of tissue-speciﬁc expression doc

... aa 60 aa 38 aa 16 aa5828 bp 3262 bp23 aa 60 aa 38 aa 16 aa7629 bp 6815 bp 7470 bp23 aa 60 aa 38 aa 16 aa1959 bp 3453 bp 1222 bp23 aa 60 aa 38 aa 16 aa554 bp 2277 bp 4313 bp23 aa 60 aa ... showing similarityto the zebraﬁsh CRABPIa and the mammalian CRAB-PIs (Fig. 1A) . The structure and coding capacity of this gene was identical to that of the mammalian and A BExon 1 Intron 1 Exon ... 38 aa 16 aa 511 bp 2925 bp 4328 bpFig. 1. Alignment of the amino acid sequences of the ﬁsh and mammalian CRABPIs and comparison of their gene structures. (A) Zebraﬁsh(Zf) CRABPIa (GenBank accession...

Báo cáo khoa học: Regulation of the expression and subcellular localization of the taurine transporter TauT in mouse NIH3T3 ﬁbroblasts doc

... antibody against acetylated a- tubuline and visualized with Alexa 488. TauT (red) was marked with a primary antibody raised against the C-terminus of TauT and visualized with Alexa 568. The merged ... against the N-terminalpart of TauT, and immunolocalization of the antibodyraised against the C-terminal part of TauT was abolished by a speciﬁc blocking peptide to t his antibody (data notshown).Besides ... fractions were separated by SDS/PAGE (10%). TauT was v isualized with a primary antibody raisedagainst the C-terminus of TauT and a secondary, alkaline, phosphatase conjugated antibody. The cytosolic...

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