Báo cáo khoa học: Val216 decides the substrate specificity of a-glucosidase in Saccharomyces cerevisiae doc

... maltose cannot bind to the active site because the side chain of Val216 interfere with binding of a 4-linked glucose. The CG1 of Val216 is too close to the O3¢ of maltose. On the other hand, both ... were used as substrates, the enzyme activity was determined as the rate of hydrolysis of the substrate by measuring the release of glucose according to the...

Ngày tải lên: 07/03/2014, 16:20

... nkatÆmg )1 . The GLD1 pro- tein was tagged with a histidine tag at the N-terminal end by adding the coding sequence for six histidines to the end of the open reading frame, and then expressed in S. cerevisiae. ... cerevisiae. The tagged protein had a similar activ- ity in the crude extract as the nontagged protein, indi- cating that the tag did not affect the prote...

Ngày tải lên: 19/02/2014, 06:20

... appear in the train- ing texts, the semantic class of each noun phrase 3 , plus a few additional features to represent the tense of the main verb phrase in the sentence and whether the document ... not the focus of the ar- ticle. We will refer to these documents as fleeting reference documents. Many of the fleeting reference documents in the MUC-4 corpus are tr...

Ngày tải lên: 20/02/2014, 04:20

... noteworthy in view of their coordinative potential, CeMT1 includes four histidines, whereas CeMT2 only has a terminal one. In the absence of a comprehensive analysis of the metal-binding abilities of ... effect at all on the bind- ing abilities of the Crs5 MT of S. cerevisiae [12], we decided to continue our studies with the obtained clones. Recombinant synthesis a...

Ngày tải lên: 07/03/2014, 02:20

... activation of the complex, which allow PAP to elongate the poly(A) tail [6,7]. Then, the poly(A) tail binds to the poly(A)-bind- ing protein (PABP), which brings in eIF4G, thus allowing the positioning ... arginine ⁄ glycine-rich (RGG) motifs that are characteristic of RNA-binding proteins, and an alanine-rich carboxy-terminal sequence that could be involved in protein–prote...

Ngày tải lên: 07/03/2014, 05:20

... preincubated with purified recombinant Cyp4x1 protein, resulting in the ablation of detection of the 55-kDa protein in brain (Fig. 5C). This shows that the antisera specifically detect the murine ... localization of the Cyp4x1 protein in brain showed specific staining of neurons, choroids epithelial cells and vascular endothelial cells. These data suggest an important rol...

Ngày tải lên: 19/02/2014, 07:20

... ,,(),,( 2211 −+∈ℜ∈ i D inn yxyxyxyx where x i is a feature vector in D-dimension space of the i-th example, and y i is the label of xi either positive or negative. The training of SVMs involves in minimize the ... features, the PrefixSpan mines the frequent patterns which occurs more than prede- fined minimum support in the training set and lim- ited in th...

Ngày tải lên: 20/02/2014, 12:20

... mass of the protein, as a result of cleavage at the interdomain tether that connects the two domains into which the single polypeptide chain protein is folded [42,43,45]. Thus, the inactivation of ... result of the closeness of the allyl group to the Trp residues present in the protein active site, and is probably responsible for the differential quenching of...

Ngày tải lên: 07/03/2014, 06:20

... tetracycline-resistance cassette into the HindIII site. pRS239 was obtained by inserting the tetra- cycline-resistance cassette into the HindIII site of pRS155, encoding glnK under the control of t ... limitation from the beginning (Fig. 4A; r) the resulting nif induction in the absence of additional GlnK synthesis was significantly decreased in cultures initially conta...

Ngày tải lên: 07/03/2014, 16:20

... in a wide range of proteins, including other GHs (clans E, F and J) [3] and hemo- pexin [20]. The CD spectrum of the latter protein [21] is very similar to the one observed here; therefore, the unusual ... course of these experiments (10 min). Docking experiments Docking of the oligosaccharide XXXG into the active site of OXG-RCBH from Geotrichum (Protein Data Bank 1sq...

Ngày tải lên: 19/02/2014, 05:20