Báo cáo khoa học: Differential interactions of decorin and decorin mutants with type I and type VI collagens pptx

... possible linke rbetween type VI and type II collagen [24]. In initialexperiments we studied the interaction of the variousdecorinformswithtypeVIcollageninasolidphasebinding assay. Decorin ... collagens and decorin are of paramount importance in attaining and maintainingtissue integrity.In the present study we investigate the binding properties of wild -type decorin, two decorin mutants ... [19].Previous studies indicated that biglycan interacts with type VI collagen and is involved in the o rganization of type VI collagen networks [37]. Our ﬁndings indicate that thebinding of biglycan...

Tài liệu Báo cáo khoa học: Differential effects of Mxi1-SRa and Mxi1-SRb in Myc antagonism ppt

... with Mxi1-SRa and enhances its ability toactivate the Myc promoter. Our ﬁndings suggest that Mxi1 gainsfunctional complexity by encoding isoforms with shared and distinctactivities.AbbreviationsFITC, ... (Fig. 4B).The ﬁnding of these differential functions is in line with the dogma that the proteome gains functionalcomplexity by encoding multiple isoforms of a givenprotein, with these isoforms ... predominanttranscript in the adult intestine and in the developingembryo, whereas Mxi1-SRb transcripts predominate inthe adult liver and kidney [12].In our initial description of Mxi1-SRa and itscomparison...

Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherﬁsh Misgurnus fossilis Functional consequences for the enzyme ppt

... This indicates a higherdegree of freedom of the native enzyme, i. e. higherstructural ﬂexibility, which is reﬂected in higher speciﬁcactivity [3]. The calorimetric enthalpy of denaturation of ... pattern of proportional pooling of subunits with different kinetic and thermodynamic properties of the tetrameric enzymemay result in ﬁne-tuning of the properties of skeletal LDH-A, which is in line with ... 4), which must inevitably manifest indifferentiation of the overall properties of pooledLDH-A iso-tetramers from warm and cold acclima-tions. This is in line with most of the observationssummarized...

Báo cáo khoa học: Differential recognition of heat shock elements by members of the heat shock transcription factor family ppt

... electrophoretic mobility shift assays, the bindingreaction was carried out in 16 lL of mixture containing0.2–2.0 lLofin vitro transcription ⁄ translation reactionmixture (0.9–5.8 ng of hHSF polypeptides), ... expres-sion of different hHSF target genes is dependent uponthe architecture of the HSE.ResultsHuman HSF1, HSF2 and HSF4 exhibit differential binding speciﬁcities for various HSE typesin vitro Interactions ... transcription via discontinuous gap -type and step- type HSEs, indicating that hHSF1 recognizes HSEs ina different way from yHSF [10]. In this study, we ana-lyzed in vitro binding of hHSF1, hHSF2 and...

Báo cáo khoa học: Differential effects of RU486 reveal distinct mechanisms for glucocorticoid repression of prostaglandin E2 release docx

... glucocorticoid inhibition, suggesting thatinhibition of COX-2 is critical in t he repression of prostaglandins by glucocorticoids. As cytokine-inducedCOX-2 and PGE2release are highly NF-jB-dependent ... including increasedgluconeogenesis, i ncreased blood glucose, amino and fattyacid mobilization, and loss of bone [2]. In addition,endogenous glucocorticoids p articipate in feedback inhibi-tion ... washing with NaCl/P i (PBS)containing 0.1% (v/v) Tween and incubation with biotin-ylated anti-rabbit immunoglobulins (Dako) for 1 h, cellswere incubated with ﬂuorescein isothiocyanate (FITC)-linked...

Báo cáo khoa học: Differential effects of histone deacetylase inhibitors on phorbol ester- and TGF-b1 induced murine tissue inhibitor of metalloproteinases-1 gene expression docx

... regulation of cyclin A and B1 upon TSA treatment isvia diminished activity of NF-Y-associated HAT and is mediated through CCAAT motifs; further, thediminished HAT activity is mediated by ... capability of degradingthe majority of ECM proteins [2]. Four tissue inhibi-tors of metalloproteinases (TIMPs) safeguard ECMintegrity by virtue of their ability to inhibit theMMPs [3]. The TIMPs ... both, increases its trans-activating potential and DNA-binding activity bymediating its dissociation from an inhibitory complexcontaining HDAC3, a class 1 HDAC [33–35]. HDAC3associates with...

Báo cáo khoa học: Differential expression of endogenous sialidases of human monocytes during cellular differentiation into macrophages potx

... sialidase activity against mixedgangliosides (MG, Fig. 2), suggesting that the siali-dase activity measured with mixed bovine gangliosideswas not due to the activity of Neu1. Thus, the activ-ity ... endogenous sialidaseactivity of monocytes increases as they differentiatein vitro into macrophages.The increase in activity of lysosomal sialidaseNeu1 during monocyte differentiation is greaterthan ... Institute of Human Virology, University of Maryland, Baltimore, MD, USA2 Division of Infectious Diseases, Department of Medicine, University of Maryland Medical Center, Baltimore, MD, USA3Hoˆpital...

Báo cáo khoa học: Differential expression of liver and kidney proteins in a mouse model for primary hyperoxaluria type I pdf

... Department of Proteomics and Signal Transduction, Klopferspitz, Martinsried, Germany2 Hospital Universitario Canarias, Center Biomedical Research on Rare Diseases (CIBERER) and Institute of Biomedical ... converting it into glycine. Theenzyme is present in peroxisomes and ⁄ or mitochondriain different mammalian species, with peroxisomal AGTbeing mainly responsible for the detoxiﬁcation of glyco-late-derived ... characterize this model, and provideevidence useful in substrate depletion strategies, wereport in this article a proteomic analysis of thechanges in expression levels of various enzymes of liverand...

Báo cáo khoa học: Differential binding of human immunoagents and Herceptin to the ErbB2 receptor ppt

... D’Alessio1 and Claudia De Lorenzo11 Dipartimento di Biologia Strutturale e Funzionale, Universita`di Napoli Federico II, Italy2 Dipartimento di Chimica, Universita`di Napoli Federico II, ItalyErbB2 ... a single set of identical independent bind-ing sites, using the Bindwork software supplied with theinstrument, which provided the stoichiometry of binding (nligand : protein), the change in ... Li JL,Shen GL & Vitetta ES (2002) Targeting multiple Her-2epitopes with monoclonal antibodies results in improvedantigrowth activity of a human breast cancer cell linein vitro and in vivo....

Báo cáo khoa học: Differential susceptibility of Plasmodium falciparum versus yeast and mammalian enolases to dissociation into active monomers doc

... subunit has three binding sites for the divalentcation, namely a conformational site (site I) , a catalyticsite (site II) and an inhibitory site (site III) [39–42]. Asbinding of divalent cation ... enolase: visualisation of the inhibitory metalbinding site III and potential as target for selective,irreversible inhibition. J Mol Biol 331, 653–665.42 Lee BH & Nowak T (1992) Inﬂuence of pH ... an additional possibility of a p–cation interaction[55]. The observed greater effectiveness of the imidazo-lium ion in dimer dissociation may arise from its abilityto undergo p–cation and stronger...

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