Báo cáo khoa học: Differential interactions of decorin and decorin mutants with type I and type VI collagens pptx

... possible linke r between type VI and type II collagen [24]. In initial experiments we studied the interaction of the various decorinformswithtypeVIcollageninasolidphase binding assay. Decorin ... collagens and decorin are of paramount importance in attaining and maintaining tissue integrity. In the present study we investigate the binding properties of wild -type dec...

Ngày tải lên: 07/03/2014, 16:20

... with Mxi1-SRa and enhances its ability to activate the Myc promoter. Our findings suggest that Mxi1 gains functional complexity by encoding isoforms with shared and distinct activities. Abbreviations FITC, ... (Fig. 4B). The finding of these differential functions is in line with the dogma that the proteome gains functional complexity by encoding multiple isoforms of a given pro...

Ngày tải lên: 18/02/2014, 16:20

... This indicates a higher degree of freedom of the native enzyme, i. e. higher structural flexibility, which is reflected in higher specific activity [3]. The calorimetric enthalpy of denaturation of ... pattern of proportional pooling of subunits with different kinetic and thermodynamic properties of the tetrameric enzyme may result in fine-tuning of the properties of skelet...

Ngày tải lên: 19/02/2014, 02:20

... electrophoretic mobility shift assays, the binding reaction was carried out in 16 lL of mixture containing 0.2–2.0 lLofin vitro transcription ⁄ translation reaction mixture (0.9–5.8 ng of hHSF polypeptides), ... expres- sion of different hHSF target genes is dependent upon the architecture of the HSE. Results Human HSF1, HSF2 and HSF4 exhibit differential binding specificities for...

Ngày tải lên: 07/03/2014, 00:20

... glucocorticoid inhibition, suggesting that inhibition of COX-2 is critical in t he repression of prostaglandins by glucocorticoids. As cytokine-induced COX-2 and PGE 2 release are highly NF-jB-dependent ... including increased gluconeogenesis, i ncreased blood glucose, amino and fatty acid mobilization, and loss of bone [2]. In addition, endogenous glucocorticoids p articipate i...

Ngày tải lên: 07/03/2014, 16:20

... regulation of cyclin A and B1 upon TSA treatment is via diminished activity of NF-Y-associated HAT and is mediated through CCAAT motifs; further, the diminished HAT activity is mediated by ... capability of degrading the majority of ECM proteins [2]. Four tissue inhibi- tors of metalloproteinases (TIMPs) safeguard ECM integrity by virtue of their ability to inhibit the MMPs [3...

Ngày tải lên: 16/03/2014, 18:20

... sialidase activity against mixed gangliosides (MG, Fig. 2), suggesting that the siali- dase activity measured with mixed bovine gangliosides was not due to the activity of Neu1. Thus, the activ- ity ... endogenous sialidase activity of monocytes increases as they differentiate in vitro into macrophages. The increase in activity of lysosomal sialidase Neu1 during monocyte differentiati...

Ngày tải lên: 16/03/2014, 19:20

... Department of Proteomics and Signal Transduction, Klopferspitz, Martinsried, Germany 2 Hospital Universitario Canarias, Center Biomedical Research on Rare Diseases (CIBERER) and Institute of Biomedical ... converting it into glycine. The enzyme is present in peroxisomes and ⁄ or mitochondria in different mammalian species, with peroxisomal AGT being mainly responsible for the deto...

Ngày tải lên: 23/03/2014, 03:20

... D’Alessio 1 and Claudia De Lorenzo 1 1 Dipartimento di Biologia Strutturale e Funzionale, Universita ` di Napoli Federico II, Italy 2 Dipartimento di Chimica, Universita ` di Napoli Federico II, Italy ErbB2 ... a single set of identical independent bind- ing sites, using the Bindwork software supplied with the instrument, which provided the stoichiometry of binding (n ligand : protein...

Ngày tải lên: 23/03/2014, 06:20

... subunit has three binding sites for the divalent cation, namely a conformational site (site I) , a catalytic site (site II) and an inhibitory site (site III) [39–42]. As binding of divalent cation ... enolase: visualisation of the inhibitory metal binding site III and potential as target for selective, irreversible inhibition. J Mol Biol 331, 653–665. 42 Lee BH & Nowak T (1992) I...

Ngày tải lên: 23/03/2014, 09:20