Báo cáo khoa học: GlnK effects complex formation between NifA and NifL in Klebsiella pneumoniae docx

... either NifL or NifA, or to the NifL NifA complex. In order to confirm the in vivo formation of a trimeric complex between NifL, NifA and GlnK, we coexpressed MBP -NifL, NifA and GlnK in K. pneumoniae ... of complex formation between cytoplasmic MBP- NifL and NifA under derepressing conditions. Based on those findings we conclude that in viv o compl...

Ngày tải lên: 07/03/2014, 16:20

... are known to be involved in either protein–protein interactions [61,62] or protein–RNA interactions [63]. The proposed intramolecular interaction may be an integrated step in the guanine nucleotide-exchange ... inhibits protein synthesis by binding EF-Tu–GTP and preventing the release of EF-Tu–GDP from the ribosome [24]. These antibiotics bind at the interface between domain 1 (guanin...

Ngày tải lên: 21/02/2014, 00:20

... in plasmin formation, to a decrease in plasmin binding or to a combination of the two. We demonstrate that both mAb 34D3 and mAb A10.2 inhibit plasmin binding to cells (Fig. 5A) and fibrin (Fig. 5B) in ... function. Blocking the kringle 1 lysine- binding site with monoclonal antibody 34D3 fully abolished binding and activation of Glu-plasminogen and prevented both fibrinolysis a...

Ngày tải lên: 20/02/2014, 01:20

... K + binding site [34–36]. Solvation and energetic of binding of Na + and K + to pyruvate kinase It has been hypothesized [18] that the difference between Na + and K + in the activation and inhibition ... binding sites (Fig. 2, inset). Competition between Na + or K + and Mg 2+ To gain further insight into how dimethylsulfoxide affects the competition between the monovalent ca...

Ngày tải lên: 08/03/2014, 02:20

... rystal structures are available [4,5] and amino-acid residues involved in substrate binding, metal binding, and catalysis h ave been identi®ed and studied in detail by site-directed mutagenesis ... their ability to bind a number of heparin-binding proteins [28]. In our search for allele-speci®c inhibitors based on single- nucleotide polymorphisms in target mRNA sequences using a...

Ngày tải lên: 08/03/2014, 10:20

... preprohepcidin, prohepcidin and mature hepcidin. Insert in pBT Insert in pTRG Growth on CTCK plates a Preprohepcidin a-1 Antitrypsin +++ Prohepcidin a-1 Antitrypsin +++ Hepcidin a-1 Antitrypsin ) a Colony ... After binding hepcidin, ferroportin is internalized, phosphor- ylated, ubiquitinated and degraded by hepatocytes and macrophages. [18,19]. The action of hepcidin is differ- ent...

Ngày tải lên: 16/03/2014, 01:20

... findings demonstrating a role for NF-jBin antagonizing keratinocyte proliferation and regulating epithelial cell differentiation [24,25]. In our experiments, TNF-a alone was insufficient to induce ... treated as in (A) and fixed 5 h after CHX addition. Cells were then immunostained with antibody to p65. 4¢-6- diamidino-2-phenylindole (DAPI) staining is visualized in blue and perinucl...

Ngày tải lên: 16/03/2014, 06:20

... preprocessing method for both training and decoding in English- Korean SMT. In particular, we transform a source language sentence by inserting pseudo words and syntactically reordering it to form a target ... linked (in- correctly) to their head’s corresponding words, or they can be linked to totally different words with respect to their meaning. In the preliminary experiment usin...

Ngày tải lên: 17/03/2014, 02:20

... Proceedings of EACL '99 The Treegram Index An Efficient Technique for Retrieval in Linguistic Treebanks Hans Argenton and Anke Feldhaus Infineon Technologies, DAT CIF, ... CIF, Postbox 801709, D-81617 Miinchen hans.argenton@infineon.com University of Tiibingen, SfS, Kleine Wilhelmstr.113, D-72074 Tiibingen feldhaus@sfs.nphil.uni-tuebingen.de Multiway trees (MT, ... the resu...

Ngày tải lên: 17/03/2014, 23:20

... genomes and template switching between genomes has been observed during reverse transcription, only a single genome strand is considered in the following discussion. The key steps in M-MLV and HIV-1 reverse ... larger b subunit, in addi- tion to possessing both the polymerase and RNase H domains found in the a subunit, also contains a C-ter- minal region corresponding to the v...

Ngày tải lên: 30/03/2014, 02:20