Báo cáo khoa học: 3 Cdt1 and geminin are down-regulated upon cell cycle exit and are over-expressed in cancer-derived cell lines potx

... recombinant full-length Cdt1 and geminin (HisT7 -geminin and His -geminin, amount of recombinant protein run in each lane in ng is indicated) and immunoblotted with anti -Cdt1 and anti -geminin Ig. ... and geminin mRNAs are down-regulated in G0 During the cell cycle, Cdt1 and geminin mRNA levels are mostly stable and protein levels are primary contr...

Ngày tải lên: 07/03/2014, 16:20

... triphenyl- phosphonium. (Received 27 February 20 03, revised 15 April 20 03, accepted 12 May 20 03) Eur. J. Biochem. 270, 2827–2 836 (20 03) Ó FEBS 20 03 doi:10.1046/j.1 432 -1 033 .20 03. 036 60.x (NARP), by targeting a restriction enzyme ... 36 . Grossman, L.I., Watson, R. & Vinograd, J. (19 73) The presence of ribonucleotides in mature closed-circular mitochondrial DNA. Proc.Natl...

Ngày tải lên: 20/02/2014, 11:20

... 150 mm; Dionex) and elution using a linear binary gradient: 5–65% ACN in 0.1% formic acid in 35 min, maintenance at 65% ACN for 10 min, and 65–5% ACN in 10 min. The typical operating conditions ... identified cleavage sites, and Ala was found N-terminal to 21 (33 %). Similar results were obtained for MMP-12, where Gly was found N-terminal of 30 (33 %) and Ala N-terminal of 36...

Ngày tải lên: 16/02/2014, 14:20

... point (+1) and the extended )10 and )35 are indicated (blue letters). The ribosome-binding site (RBS) and translation initiation codon (Met) of kis are underlined and shown in red. The N-terminal ... 1 43 (Pt 11), 34 03 34 16. 8 Hayes F (20 03) Toxins-antitoxins: plasmid mainte- nance, programmed cell death, and cell cycle arrest. Science 30 1, 1496–1499. 9 Yarmolinsk...

Ngày tải lên: 18/02/2014, 04:20

... constitutively in cells and binds to and regulates the expression of genes without any apparent stress intervention [1 ,3] . HSF2 and HSF4 may not be directly involved in the stress response, but rather in cell ... DNA-binding domain (DBD) at the amino-terminus and the oligomerization domain in the central region of the protein [1,4]. The HSF DBD belongs to the ‘winged’ heli...

Ngày tải lên: 18/02/2014, 04:20

... of colorectal cell lines and NIH3T3 cells overexpressing miR-192 ⁄ 194. NIH3T3) is the control cell line and NIH3T3+ indicates the cells overexpressing miR-192 ⁄ 194. (C) Graph showing the periodicity ... cluster in NIH3T3 cells to identify its effect on the circadian cycle. In NIH3T3 cells, miR-192 and miR-194 are almost undetectable (Fig. 5A). As expected, the intro- duct...

Ngày tải lên: 18/02/2014, 06:20

... class of RNA-binding proteins. RNA 3, 1421–1 433 . 35 Cheong CG & Hall TM (2006) Engineering RNA sequence specificity of Pumilio repeats. Proc Natl Acad Sci USA 1 03, 136 35– 136 39. 36 Murata Y & ... the binding of RNA-binding proteins to 3 UTR tran- scripts has been poorly described in plants. PUF proteins are a large family of RNA-binding proteins found in all eukaryotes....

Ngày tải lên: 18/02/2014, 06:20

... containing over 20 grouped PS molecules). We have shown that doubling the PS concentration in the membrane results in an increase in the number of binding sites (compare Fig. 3D and 3E) and also in ... ⁄ Q32E ⁄ N33D (QNED); mutant III, P10Q ⁄ S12N ⁄ D23S ⁄ Q32E⁄ N33D (QNSED); mu- tant IV, P10Q ⁄ S12N ⁄ D23S (QNS); mutant V, Q32E ⁄ N33D (ED); mutant VI, D23S (S); and mutant VII,...

Ngày tải lên: 18/02/2014, 06:20

... Several hemicellulases were identified, including three xylanases in F1, and several cellulases belonging to glycoside hydrolase families 9 and 5 and, a cystein protease inhibitor were identified in F5 and ... cluster, beginning with the gene encoding the scaffolding pro- tein named cellulosome-integrating protein C (CipC), followed by genes that code for the major cellulosomal cellu...

Ngày tải lên: 18/02/2014, 08:20

... 64.0 33 .3 ) 30 .7 ) 3. 7 ) 40.6 ) 36 .8 m 67.8 73. 9 6.1 25 p 65.8 33 .2 ) 32 .6 ) 2 .3 ) 40.6 ) 38 .2 m 68.1 73. 8 5.7 30 p 65.5 33 .2 ) 32 .3 ) 1.9 ) 40.6 ) 38 .7 m 67.4 73. 7 6.4 37 p 66.1 33 .1 ) 32 .9 ... N-terminus (Thr and Met) are encoded by the expression vector. An analysis of the amino-acid composition shows that VsEndA contains 13 more lysines and two fewe...

Ngày tải lên: 19/02/2014, 05:20