Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx

... zwit- terionic amino acid substrates and protons with a stoichio- metric coupling of 1 : 1 as shown for the model substrate L -proline [2]. Substrates of both transporters are the small apolar amino acids ... Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 Martin Foltz, Carmen Oechsler, Michael Boll, Gabor...

Ngày tải lên: 07/03/2014, 16:20

... °C. The activation energy (E a ) was determined from the slope ()E a ⁄ R) of Arrhenius plots of log V (nmol of product per minute) versus 1 ⁄ T. Substrate specificity The substrate specificity of ... determined by the Bradford method [37] using the Sigma prepared reagent and BSA as the standard. Effects of divalent metal ions and thiols on BH activities The e...

Ngày tải lên: 18/02/2014, 14:20

... obtained with the transition at posi- tions 1915 and 1917. These mutations had an effect on the formation of pseudouridine exclusively at the mutant position and did not affect either of the other two ... of the wild-type 23S rRNA from the 50S and the 70S particles were similar: stop sites were found at positions 1911 and 1917 on the CMCT treated RNA and not on...

Ngày tải lên: 18/02/2014, 16:20

... were detected in the soluble fraction, and the levels of both were signifi- cantly increased by HU treatment, but the amount of neither of them was dependent on the presence of Hat1. Although ... H4 associated with the HAT-B enzyme from the yeast soluble fraction. The requirements for the distinct components of the complex for the acetyla- tion and the assoc...

Ngày tải lên: 07/03/2014, 05:20

... from low amounts of pantoate [9]. Here, we report the results of the structural studies of the panto- ate-bound form of the N-terminal domain of E. coli PS (nPS). The structure and substrate- binding ... molecule takes the position of the adenine ring of ATP. The carbon and oxygen atoms of pantoate are shown in yellow and red, respectively. The carbon...

Ngày tải lên: 16/02/2014, 09:20

... percentage intensity of the band of the 57-kDa protein in a total of intensities of the 57-kDa, 38-kDa and 19-kDa bands, where the intensity of the 38-kDa protein band was corrected for the amounts of the added ... sizes. The rates of ligation in the absence of added proteins were taken as 100%. The mean of the values of three independent experiment...

Ngày tải lên: 19/02/2014, 07:20

... binding of the methionine sulfoxide residue at the S 1 ¢ subsite of the active centers of NE and Pr3. The fact that the S 1 ¢ subsite of Pr3 is significantly smaller than that of NE [21] might then explain ... conclude that the oxidant-promoted alteration of the K i of elafin and trappin is caused by the oxidation of their P 1 ¢ methionine residue. Fig. 2. S...

Ngày tải lên: 19/02/2014, 07:20

... methylation [8], and the cleavage of DNA by alkali [31]. The method allows easy detection of DNA–protein interactions via the N7 position of guanines in the major groove and via the N3 position of adenines ... structure and gene activity. The MMTV-LTR contains potential regulatory elements which mediate transcription in the presence of glucocorti- coid ligands a...

Ngày tải lên: 19/02/2014, 12:20

... splicing and evolution With increasing understanding of the importance of cis regulatory sequences located either in the introns or in the coding sequence for the splicing process, the scien- tific ... the inclusion of a new exon in only a minor fraction of the mature transcript represent the best candidates in the creation of new splicing substrates for evolution...

Ngày tải lên: 06/03/2014, 09:22

... immunogenicity of the antibody framework from a different species [23–25]. The success of the series of studies shows that the stable framework structure of Fv enables the transfer of function by means of ... binding affinity and specificity. The strong molecular recognition of antibodies plays important roles in the immune system, and it has been applied in the...

Ngày tải lên: 06/03/2014, 11:20