Báo cáo khoa học: Akt-dependent phosphorylation negatively regulates the transcriptional activity of dHAND by inhibiting the DNA binding activity pdf

... speculated that phosphorylation of these sites might affect the DNA binding and/or dimerization activities of dHAND. We first analyzed the effect of phosphorylation on the transcriptional activity of dHAND ... the DNA binding activity of dHAND could be affected by Akt phosphorylation. The influence of phos- phorylation by Akt on the DNA bin...

Ngày tải lên: 07/03/2014, 16:20

... conditions [22], but with the wall of the pore made of both lipid and pro- tein molecules. At the rim of the membrane hole, the two leaflets of the bilayer can make contact with each other, forming a membrane ... permeabilizing activity of a pep- tide derived from the second helix of the putative pore-forming domain of Bax. We show that peptides with the seque...

Ngày tải lên: 19/02/2014, 07:20

... blots of lysates of Y394F tau (Fig. 2A). The absence of this larger species could indi- cate that phosphorylation of tau by Fyn is reduced and therefore, in addition to phosphorylation by c-Abl [25], ... interaction is regulated by serine ⁄ threonine phosphorylation of tau [20,21]. To determine whether the tyrosine phosphorylation status of tau also affects the...

Ngày tải lên: 14/02/2014, 14:20

... within the b-barrel, induced by the disulfide bridge, have been mapped onto the BABP apo structure, and the effects of the binding of the two most abundant glycoconju- gated bile salts on the backbone ... GCDA. The comparison of 1 H-spectra of the two protein samples at a pro- tein ⁄ GCDA ⁄ GCA ratio of 1 : 2 : 2 indicated that the final holo state is independe...

Ngày tải lên: 18/02/2014, 06:20

... suggesting that phosphorylation of these sites might regulate the formation of the SF1-U2AF-RNA complex. We first tested the effect of phosphorylation on the interaction of SF1 with U2AF 65 by GST pull-down ... in the presence of 1.2 l M U2AF 65 . The different bands were quantified by phosphorimaging and the formation of the tern- ary complex was plotted as...

Ngày tải lên: 19/02/2014, 07:20

... if the binding of one affects the binding of the other. We report here that phosphorylation of Tyr304 in the functional C-terminal region (residues 301–333) of ephrin B2 confers high-affinity binding ... affinity of the binding interactions was evaluated by measuring the changes of fluorescence polarization of the peptides at each step of titration with t...

Ngày tải lên: 19/02/2014, 12:20

... from the intestinal digestion of proteins [4,5]. Another example of the detection of the lack of an amino acid is metabolic adaptation to cope with epi- sodes of protein malnutrition. In these ... in bold). The enrichment for a given GO category was computed as the ratio of the distribution of the amino acid-regulated genes* versus the distribution of the gen...

Ngày tải lên: 07/03/2014, 03:20

... been the subject of intensive research for many years. Yet, we don’t know the details of the molecular dysregulation in the target cells of the hormone. Studies of cells from patients with the ... tyrosine- phosphorylation of the insulin receptor or of IRS1, or of phosphorylation of ERK1 ⁄ 2, was not significantly different in either group of diabetics compa...

Ngày tải lên: 07/03/2014, 16:20

... counter. Values are the mean of six replicates. The specific uptake was determined by subtracting the uptake counts in the absence of inhibitor from the uptake counts in the presence of cocaine or S-cita- lopram, ... with the direct binding of p25a to vesicles suggest the pres- ence of vesicular p25a receptors, and this is now under investigation. The functional...

Ngày tải lên: 14/02/2014, 21:20

... stress) of adipose tissue. These findings support the possibility that the alteration of circulating ANGPTL2 levels could serve as a marker of obesity-induced metabolic abnormalities. Furthermore, ... adipose tissue of ANGPTL2 null mice compared with that of wild-type mice [15]. These results raise the possibility that blocking ANGPTL2 signaling simultaneously sup- presses the...

Ngày tải lên: 14/02/2014, 22:20