Báo cáo khoa học: The in vitro effects of CRE-decoy oligonucleotides in combination with conventional chemotherapy in colorectal cancer cell lines potx

... in blue, oxygens in red, hydrogens in grey. Carbon atoms of Pro are coloured in cyan, those of other amino acids in green. The side chain methyl carbon of b-HAla and the pro R methyl carbon of ... phospho- lipase C (PLC) in CHO cells transfected with the human NK-1 receptor [24,25]. Modelling and superimposition of the conformers of the different b-anda-ami...

Ngày tải lên: 08/03/2014, 08:20

... frugiperda (Sf)9 cells expressing the recombinant mPC1 ⁄ 3, the presence of the 87 kDa form in excess of the 66 ⁄ 71 kDa facilitates isolation of the enzyme and helps in maintaining the enzymatic ... substrate (pERTKR–MCA) in the presence of increasing concentrations from 0to10l M of RP-HPLC purified CT-peptide. The control condition corresponds to incubation o...

Ngày tải lên: 30/03/2014, 08:20

... are involved in the correct folding of proteins and ⁄ or the removal of incompletely synthes- ized, damaged or malfolded proteins in the different compartments of the cell [1–4]. By studying the ... membrane cell wall interface [6]. Thereafter, they can pass the cell wall in order to be released into the growth medium. During these post-translocational sta- ges...

Ngày tải lên: 07/03/2014, 12:20

... sorts of conceptual relationships that can occur in the "layered" domains of interest [1]. Finally, the macro file contains the meanings of modifiers, roughly in the form in which they ... MODIFIERS The job of the knowledge acquisition module of LDC, called "Prep" in Figure 1, is to' find out about (a) the vocabulary of the new d...

Ngày tải lên: 17/03/2014, 19:21

... confirmed the results obtained in the pull-down assays, showing that there is a physical interaction between the N- and C-terminal domains of SSIII in vitro. Mapping of the CD-binding region in the N-terminal ... D3 domains, respectively, but not the individual starch-binding domains, are involved in the interaction with the catalytic domain. We also determined...

Ngày tải lên: 22/03/2014, 21:20

... involved in the regulation via interactions with the different proteins operating in the cascade, resulting in an integration of the various signals. Indeed, UTase appears to monitor the gluta- mine ... activity of the protein at the bottom of the hierarchy depends on the modification state of that protein. The advantage of modulating the activity of a...

Ngày tải lên: 23/03/2014, 04:21

... and the fate of its two subunits, RTA and the cell- binding galactose-specific lectin ricin toxin B chain (RTB), are essential to gain further insights into the mechanism of toxin action [1]. During ... polypeptide of the heterodimeric toxin ricin, which is produced in the endosperm of the seed of the castor bean plant, Ricinus communis. The study of ricin, in...

Ngày tải lên: 23/03/2014, 07:20

... As seen in Fig. 6A, the position of the catalytic Asp214 residue is fixed with the interaction to (a) the e-amine of Lys78 and (b) the imidazole ring of His34. Asp214 also inter- acts with a water ... interaction between the main chain oxygen of Ala216 and the e-amine of Lys78. In H34A mutant (Fig. 6B), the interaction between the imidazole ring and Asp214 d...

Ngày tải lên: 23/03/2014, 07:20

... plated in media containing Geneticin to select for cell containing vector DNA (as in Fig. 5A,G). After three weeks, the number of cells was determined by fixing cells and staining with dye. Individual ... in vitro. Cellular stress protein responses play a key role in minimizing cell injury and maintaining tissue integrity in response to damaging levels of an environment...

Ngày tải lên: 23/03/2014, 10:21

... establish if the lack of processing and dissociation of the A. nidulans IAT was due to intrinsic characteristics of the protein itself or to the absence of a processing endopeptidase in the host, the ... a 1.6-kb XhoI–XbaI probe containing the penDE gene. The absence of introns was confirmed by sequencing clones containing fragments covering the intron sites; the...

Ngày tải lên: 31/03/2014, 01:20