Báo cáo khoa học: Kinetics of the inhibition of neutrophil proteinases by recombinant elafin and pre-elafin (trappin-2) expressed in Pichia pastoris ppt
Tài liệu Báo cáo khoa học: Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane pdf
... repre-sent the last subunits incorporated into the bc1com-plex. On the other hand, the absence of Qcr9p in the DQCR9 ⁄ DQCR10 strain prevented the binding of ISP(Fig. 4B). Interestingly, in the ... addition of ISP and Qcr10p).On the other hand, in the transition from the 500 kDaband to the 670 kDa band, a structural rearrangement of the bc1complex may occur due to the binding of ISP and ... comparable amounts in both yeast strains. Therefore, the reason for the disap-pearance of the bc1dimer in the yeast strain in whichQcr10p is missing remains unknown.On the basis of all the above...
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Tài liệu Báo cáo khoa học: Looking for the ancestry of the heavy-chain subunits of heteromeric amino acid transporters rBAT and 4F2hc within the GH13 a-amylase family ppt
... is the lack of domain B protruding out of the TIM barrel in the place of loop 3 connecting the b3-strand to the a3-helix [9,21]. Sharing domain B by rBATs and GH13 enzymes, and especially the ... barrel domain,including domain B and the C-terminal domain C forrBAT proteins (669 residues on average); (b) the cata-lytic TIM barrel domain, including domain B and the C-terminal domain C for ... in region I covering the b3-strand; (b) a segment of up to five residues(307_GVDGF in human rBAT) preceding the func-tional arginine (arginine 197 in oligo-1,6-glucosidase) in region II of the...
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Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt
... similar-ity in the remaining parts of the molecules. Most mam-malian PLDs contain a PX and a PH domain instead of the C2 domain in plants, whereas microbial PLDslack such probably regulatory domains.Despite ... responsible for the calcium binding [8], we hypothesize that this bind-ing event takes place at the C2 domain.A second calcium-binding site of lower affinity at the C2 domain (corresponding to the dissociationconstant ... inactivated than in the absence of higher salt concentrations. In Table 1, the rate constants of irreversible inactivation, which fol-lowed first-order kinetics, in the absence and presenceof...
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Tài liệu Báo cáo khoa học: Mixed-type noncompetitive inhibition of anthrax lethal factor protease by aminoglycosides ppt
... governing the inhibition of the lethal factor protease by aminoglycosides. Forexample, the kinetic mechanism of inhibition by neo-mycin B has been reported as being competitive with the substrate ... binding. Only one ion pair seems to beinvolved in the binding process, which suggests the presence of a singlebinding site. Combining the results of our substrate competition studieswith the ... substrate, in conjunctionwith the mixed-type noncompetitive inhibition patternseen with neomycin B and other inhibitors, suggesteither the presence of two distinct binding sites or the involvement of...
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Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf
... hypoxia on HO-1 and HO-2 expression in human cell linesWe initially analyzed the effects of hypoxia on the expression of HO-1 and HO-2 in human cell lines of bone marrow origin, including KG1 myeloid ... the intensity for the a-tubulin signal. Shown are the relativeexpression levels of HO-1 and HO-2 proteins in YN-1 cells (B) and those of HO-2 protein in K562 cells (D). The intensity representing ... C). Other conditions are described in Fig. 1B. The intensities of the signals in (A) and (C) were quantified, and the intensity representing HO-1 or HO-2 protein was normalized with respect to the...
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Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... ana-lysis of the effect was not performed, the inhibition by 20 mm agmatine was eliminated by saturation withl-arginine, indicating the competitive character of the inhibition produced by the substrate ... known to be involved in binding and hydrolysis of the guanidino group of l-arginine by arginase are strictly conserved in the act-ive site of the agmatinases [19]. Moreover, modelingstudies ... effect of agmatine on arginine hydrolysis, reactions were followed by measuring the formation of ornithine, determined by the method of Chinard [42]. Protein concentrations weredetermined by the...
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Báo cáo khoa học: Studies on the role of the receptor protein motifs possibly involved in electrostatic interactions on the dopamine D1 and D2 receptor oligomerization pdf
... protein 4.1 N, a member of the 4.1 family,facilitates the transport of D2R to the cell surface by interacting with the N-terminal portion of the ic3 loop of D2R via its C-terminal domain [66]. ... Measuring receptor dimer-ization by monitoring changes in the fluorescence life-time of probes linked to the receptors of interest seems the best approach in this kind of the study. Although the ... GavÞ2rð4Þwhere Ri and Gi are the red and green intensities of voxelI, respectively, and Rav and Gav the average values of Ri and Gi, respectively.It is used for describing the correlation of the intensitydistributions...
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Báo cáo khoa học: Investigations into the ability of an oblique a-helical template to provide the basis for design of an antimicrobial anionic amphiphilic peptide pot
... using the methodology of Simpson [58]. The interaction parameter (a) relates the interaction of each molar fraction of components within a monolayerwith the free energy of mixing. Values of ... valuesfor DGMix, a and DH in the absence of AP1, indicatingmembrane stability, but, in the presence of AP1, posit-ive values of for DGMix, a and DH were obtained, sug-gesting that the monolayer ... treats the hydrophobicity of successive amino acids in a sequence, asvectors and then sums these vectors in two dimensions,assuming an amino-acid side chain periodicity of 100 °. The resultant of...
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Báo cáo khoa học: Flexible nets The roles of intrinsic disorder in protein interaction networks potx
... to the regions of the proteininvolved in interaction with Cdk2 and cyclin A: 1, domain 1 interacting withcyclin A; 2, a linker helix involved in bindingboth cyclin A and Cdk2; 3, domain 2 inter-acting ... Cdk2,p27Kip1binds and rearranges the amino-terminal lobe and also inserts into the catalytic cleft, mimicking ATP in the context of the cyclin A–Cdk2 complex [120]. In contrast, the unbound p27Kip1Cdk -inhibition ... (as esti-mated by a method suggested in [130]). The binding of p27Kip1to Cdk2 leading to the inhibition of kinaseactivity is much slower and is accompanied by the fold-ing of 59 residues...
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Báo cáo khoa học: Evidence for the presence of ferritin in plant mitochondria pdf
... assayed in the presence and absence of antimycin Ato assess the contamination from endoplasmic reticulum. In purified mitochondria (in the presence of antimy cin A), the activity was 4.35 times and ... partiallylost the cytochrome c; the densitometric a nalysis of the immunodecoration show a d ecrease of approximately 50% in the Mpl proteins. On the other hand, the immunodec-oration of PMt and Mpl ... drops of blocking buffer: 1% (w/v) bovine serumalbumin, 20% (v/v) normal goat serum in 0.1MTris-buffered saline (pH 7.4), and then incubated for 2 h in Tris-buffered saline (pH 7.4) containing...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpBáo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Báo cáo quy trình mua hàng CT CP Công Nghệ NPVchuyên đề điện xoay chiều theo dạngNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Nghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀM