Báo cáo khoa học: Kinetics of the inhibition of neutrophil proteinases by recombinant elafin and pre-elafin (trappin-2) expressed in Pichia pastoris ppt
Tài liệu Báo cáo khoa học: Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane pdf
... repre- sent the last subunits incorporated into the bc 1 com- plex. On the other hand, the absence of Qcr9p in the DQCR9 ⁄ DQCR10 strain prevented the binding of ISP (Fig. 4B). Interestingly, in the ... addition of ISP and Qcr10p). On the other hand, in the transition from the 500 kDa band to the 670 kDa band, a structural rearrangement of the bc 1 comp...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: Looking for the ancestry of the heavy-chain subunits of heteromeric amino acid transporters rBAT and 4F2hc within the GH13 a-amylase family ppt
... is the lack of domain B protruding out of the TIM barrel in the place of loop 3 connecting the b3-strand to the a3-helix [9,21]. Sharing domain B by rBATs and GH13 enzymes, and especially the ... barrel domain, including domain B and the C-terminal domain C for rBAT proteins (669 residues on average); (b) the cata- lytic TIM barrel domain, including domain B a...
Ngày tải lên: 18/02/2014, 13:20
Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt
... similar- ity in the remaining parts of the molecules. Most mam- malian PLDs contain a PX and a PH domain instead of the C2 domain in plants, whereas microbial PLDs lack such probably regulatory domains. Despite ... responsible for the calcium binding [8], we hypothesize that this bind- ing event takes place at the C2 domain. A second calcium-binding site of lower affinity...
Ngày tải lên: 19/02/2014, 02:20
Tài liệu Báo cáo khoa học: Mixed-type noncompetitive inhibition of anthrax lethal factor protease by aminoglycosides ppt
... governing the inhibition of the lethal factor protease by aminoglycosides. For example, the kinetic mechanism of inhibition by neo- mycin B has been reported as being competitive with the substrate ... binding. Only one ion pair seems to be involved in the binding process, which suggests the presence of a single binding site. Combining the results of our substr...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf
... hypoxia on HO-1 and HO-2 expression in human cell lines We initially analyzed the effects of hypoxia on the expression of HO-1 and HO-2 in human cell lines of bone marrow origin, including KG1 myeloid ... the intensity for the a-tubulin signal. Shown are the relative expression levels of HO-1 and HO-2 proteins in YN-1 cells (B) and those of HO-2 protein in...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... ana- lysis of the effect was not performed, the inhibition by 20 mm agmatine was eliminated by saturation with l-arginine, indicating the competitive character of the inhibition produced by the substrate ... known to be involved in binding and hydrolysis of the guanidino group of l-arginine by arginase are strictly conserved in the act- ive site of t...
Ngày tải lên: 20/02/2014, 02:21
Báo cáo khoa học: Studies on the role of the receptor protein motifs possibly involved in electrostatic interactions on the dopamine D1 and D2 receptor oligomerization pdf
... protein 4.1 N, a member of the 4.1 family, facilitates the transport of D 2 R to the cell surface by interacting with the N-terminal portion of the ic3 loop of D 2 R via its C-terminal domain [66]. ... Measuring receptor dimer- ization by monitoring changes in the fluorescence life- time of probes linked to the receptors of interest seems the best approach in...
Ngày tải lên: 07/03/2014, 03:20
Báo cáo khoa học: Investigations into the ability of an oblique a-helical template to provide the basis for design of an antimicrobial anionic amphiphilic peptide pot
... using the methodology of Simpson [58]. The interaction parameter (a) relates the interaction of each molar fraction of components within a monolayer with the free energy of mixing. Values of ... values for DG Mix , a and DH in the absence of AP1, indicating membrane stability, but, in the presence of AP1, posit- ive values of for DG Mix , a and DH were obta...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Flexible nets The roles of intrinsic disorder in protein interaction networks potx
... to the regions of the protein involved in interaction with Cdk2 and cyclin A: 1, domain 1 interacting with cyclin A; 2, a linker helix involved in binding both cyclin A and Cdk2; 3, domain 2 inter- acting ... Cdk2, p27 Kip1 binds and rearranges the amino-terminal lobe and also inserts into the catalytic cleft, mimicking ATP in the context of the cyclin A–Cdk2 com...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Evidence for the presence of ferritin in plant mitochondria pdf
... assayed in the presence and absence of antimycin A to assess the contamination from endoplasmic reticulum. In purified mitochondria (in the presence of antimy cin A), the activity was 4.35 times and ... partially lost the cytochrome c; the densitometric a nalysis of the immunodecoration show a d ecrease of approximately 50% in the Mpl proteins. On the other...
Ngày tải lên: 16/03/2014, 18:20