Báo cáo khoa học: Glycosphingolipids in Plasmodium falciparum Presence of an active glucosylceramide synthase pot

... timethe presence of an active glucosylceramide synthase in theintraerythrocytic stages of P. falciparum. Incorporation of [14C]palmitic acid and [14C]glucose allowed the analysis of puriﬁed glycosphingolipids. ... results obtained by TLC analysis of the long chain bases. Presence of an active glucosylceramide synthase In a ﬁrst approach, the activity of the enzyme in a parasitelysate was examined using UDP-[14C]glucose ... of an authentic standard of C18-sphinganine, the other one (RF0.38) migrating slightlyabove, would correspond to C20-sphinganine. A similarresult was obtained when the spot comigrating...

Báo cáo khoa học: Identiﬁcation and expression analysis of an IL-18 homologue and its alternatively spliced form in rainbow trout (Oncorhynchus mykiss) doc

... c.secombes@abdn.ac.ukAbbreviations: IL, interleukin; IL-1Ra, interleukin 1 receptorantagonist; ICE: interleukin 1b converting enzyme; IL)18 BP,interleukin 18 binding protein; IFN, interferon; c-IP10, interferongamma induced ... alternativesplicing may play an important role in regulating IL-18activities in rainbow trout.Keywords: interleukin 18; alternative splicing; expression;rainbow trout. In the last few years, major advances ... skinand spleen, using TRIZOL (Invitrogen) according to themanufacturer’s instructions. The RNA was then reversetranscribed into cDNA using Bioscript (Bioline) accordingto the manufacturer’s instructions....

Báo cáo khoa học: "Studies in Machine Translation—8: Manual for Postediting Russian Text" pot

... articles a and an are not distinguished. Connections: of, to, by, with, than, as, in, on. English connecting words must be inserted in the absence of a Russian equivalent, in two kinds of context ... by the insertion of accurate entries in the coding space. When the computer performs an inflection, it also prints a mark in the coding space. 3.2 ENGLISH INFLECTIONS The posteditor inflects ... text listing prepared on an IBM printer; a sample list is shown in Table 2. Each occurrence in the Russian text occupies one line of the listing; the following items of information are given...

Báo cáo khoa học: "Search in the Lost Sense of “Query”: Question Formulation in Web Search Queries and its Temporal Change" pptx

... context and an improved un-derstanding of question-queries by search engines.2 Related workThere has been some work on studying and exploit-ing linguistic structure in web queries. Spink andOzmultu ... to the in- crease in Q-level, if we examine the co-click datawithout the stability constraint, we observe the fol-lowing: an increasing click trafﬁc from Q-queriesand an increasing gap in click ... onecan observe how the fraction of query trafﬁc con-taining the phrase1changes over time. For instance,as shown next, the fraction of query trafﬁc contain-ing “how to” has in fact been going...

Báo cáo khoa học: Alterations in the photoactivation pathway of rhodopsin mutants associated with retinitis pigmentosa potx

... Structure andfunction in rhodopsin: correct folding and misfolding in point mutants at and in proximity to the site of theretinitis pigmentosa mutation Leu-125–>Arg in thetransmembrane helix ... A, Park JH, Choe HW,Ernst OP, Hofmann KP & Hildebrand PW (2009)Structural and kinetic modeling of an activating helixswitch in the rhodopsin–transducin interface. Proc NatlAcad Sci USA ... the results obtained in DMsolution.The altered band of these mutants, in DM solution,had less intensity than the corresponding band formed in the case of the G51V single mutant. t1 ⁄ 2for...

Báo cáo khoa học: The in vitro nuclear aggregates of polyamines pot

... separation of polyaminesolutions prepared by changing the concentration of a single poly-amine and keeping the concentrations of the other two constant(24 mM). In the case of variation of Spm ... were obtained by progres-sively increasing (in the range 5–48 mM) the concentration of (A)Spm, (B) Spd and (C) Put, keeping the concentration of the remain-ing two polyamines constant at 24 ... report ﬁndings indicating struc-tural and functional analogies among extractive andsynthetic NAPs: therefore, according to their mole-cular masses, and in keeping with the terminology of natural...

Báo cáo khoa học: Identiﬁcation and functional characterization of an aggregation domain in long myosin light chain kinase ppt

... barrier in intestinal disease.Lab Invest 84, 282–291.12 Tran QK, Watanabe H, Zhang XX, Takahashi R &Ohno R (1999) Involvement of myosin light-chainkinase in chloride-sensitive Ca2+ in ux in ... Research Center, Nanjing University, China2 School of Life Science, Nanjing Normal University, China3 National Institute of Biological Science, Beijing, ChinaMyosin light chain kinases (MLCKs) ... molecules and in intracellular cytoskeletalproteins, including titin, MyBP-Cm, MyBP-M, MyBP-H, myotilin, and palladin [30–35]. As Ig-C2 domainsmay serve as molecular spacers and bind to a diversityof...

Tài liệu Báo cáo khoa học: Physico-chemical characterization and synthesis of neuronally active a-conotoxins docx

... of cholecystokinin-12 and bradykinin containing tyrosinesulfate [57], and could perhaps be applicable to synthesis of a-conopeptides.Synthesis of variants of a-conotoxins: alanine scansand loop replacements In ... reports of the solid phase synthesis of sulfotyrosine-containing a-conotoxins including EpI,PnIA, PnIB, AnIA, AnIB and AnIC and some ana-logues [21,24,56]. Most of the reported syntheses of PnIA, ... well-characterized peptides(some of them including a-conotoxin SI) and proteins. One of the original comprehensive studies of disulﬁde bondlinkage relevant to conotoxins involved analysis of differ-entially...

Báo cáo khoa học: Cell biology, regulation and inhibition of b-secretase (BACE-1) potx

... amember of the ‘a disintegrin and metalloprotease’(ADAM) family of proteins [31]. Shedding is a processby which many integral membrane proteins, such asangiotensin-converting enzyme and tumour ... simultaneously by a number of independent groups using quite distinct methodologies.It is unique in being a transmembrane asparticprotease of type I topology, in which the N-terminusand catalytic ... overex-pression of soluble BACE-1 resulted in a dramaticincrease in the production of Ab, and so membraneanchorage in the vicinity of its substrate is notessential.Expression and localization of BACEBACE-1...

Báo cáo khoa học: NMR and molecular dynamics studies of an autoimmune myelin basic protein peptide and its antagonist Structural implications for the MHC II (I-Au)–peptide complex from docking calculations ppt

... indicates the important role of Arg78, ﬁrstly in thestabilization of local microdomains (e pitopes) of the integralprotein and in a number of post-translational modiﬁcation srelevant to multiple ... University of Ioannina, Greece;4Department of Chemistry, University of Patras, GreeceExperimental autoimmune encephalomyelitis can beinduced in susceptible animals by immunodominant deter-minants ... structuralimportance of the nature o f the amino acid at position 81 of the encephalitogenic sequence 74–85 of guinea MBP:replacement of Asp81 with an alanine seems to break achain of electrostatic interactions,...

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