Báo cáo khóa học: Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase docx

... reports on the direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase (DgAOR), a molybdenum enzyme of the xanthine oxidase family that contains three redox-active cofactors: ... [16,20]. Electrochemistry of Dg AOR in the presence of aldehydes Cyclic voltammetry was used to analyze the behavior of DgAOR in the presence of increasing con...

Ngày tải lên: 07/03/2014, 15:20

... ancestor of life. With respect to the studied enzymes of the POP family, POP seems to be the most conserved enzyme. Ten conserved amino acids were found at the active site of the enzyme of each of the ... proposed that the b-propeller of P. furiosus POP does not perform the same function as the mammalian enzyme, i.e. the exclusion of large peptides from the...

Ngày tải lên: 19/02/2014, 13:20

... active sites. Refolding of the scrambled RNase in the presence o f P DI was used as a positive control, and the absence of the recovery of the RNase activity in the presence of the thioredoxin from Alicyclobacillus ... wasusedasanegative control. The refolding of the scrambled RNase in the presence of Pf PDO seems to be less efficient when using PDI. However, t...

Ngày tải lên: 16/03/2014, 16:20

... [26]. These findings lead to the hypothesis that Fe-bound ApeSOD mimics the product-inhibited form and the shift of Tyr39 sup- presses the release of the peroxide product. This may be one of the ... inhibi- tion of the binding of the superoxide substrate and the other to product inhibition by hydrogen peroxide. There are two mechanisms proposed for the reaction cy...

Ngày tải lên: 14/02/2014, 22:20

... man- ganese in the transcriptional regulation of the nrd operon [35] may not be considered in the light of the results obtained in the present study. Rather, the involvement of manganese in the in vivo ... RNR and that of other class 1 RNRs or, instead, is a result of the interaction of the R2F with the inactivated (oxidized) form of the R1E. We consider t...

Ngày tải lên: 15/02/2014, 01:20

... EC 2.7.2.4) is an enzyme that catalyzes the first committed step, the phosphorylation of the c-carboxyl group of aspartate, of the biosynthetic pathway of the aspartic acid group amino acids Lys, Thr, ... and B) sections of the structure are not seen on the map, probably owing to disorder of these regions. The over- all geometry of the model according to the p...

Ngày tải lên: 18/02/2014, 08:20

... that the activation of IRF-3 by M-pIC treatment is mediated by the TLR3 ⁄ TRIF signaling pathway. We obtained further evidence by examining the status of the phos- phorylation and dimerization of ... cells. First, we confirmed the effect of NS3-4A on the activation of the IFN-b gene promoter by the Cardif exogenously expressed in PH5CH8 cells. The results of the...

Ngày tải lên: 18/02/2014, 16:20

... K d is the equilibrium dissociation constant of the 5¢ exon mimic, h is the fraction of 5¢ exon mimic bound to the ribozyme, and [ribozyme] u is the concentration of unbound ribozyme in the reaction. Measurement ... and fitting to the equation: k obs ¼ k 1 ½Eþk À1 : Measurement of the dissociation constant, K d P of the ribozyme–product complex The equilibrium di...

Ngày tải lên: 18/02/2014, 18:20

... according to the residue in the mid- point of the respective loop, as shown in Fig. 2. To the east of the active site the 37- and 60-loops border the S2¢ pocket of the proteinase. The observed ... below). The southern boundary of the active site cleft of DESC1 is formed by the 145 autolysis loop. The backbone of this loop differs mark- edly from the oth...

Ngày tải lên: 19/02/2014, 00:20

... determines the shape of the active site entry. (e) The structure of the active site is essentially identical with the active sites of the MshB and LpxC proteins. The conservation of catalytically important ... formation. Sections (6 A ˚ thick) of the protein surface that illustrate the shape and size of the active site. The sphere represents the zinc ion. (...

Ngày tải lên: 19/02/2014, 00:20