Báo cáo khóa học: Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using fluorescence spectroscopy pptx
Báo cáo khóa học: Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using fluorescence spectroscopy pptx
... transition in AC (Eur. J. Biochem. 271) 833 Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using fluorescence spectroscopy Jacques Gallay 1 , Michel Vincent 1 , ... direct exploitation of the structural results of the former to explain the mechanism of CaM activation of the latter. Crystallizatio...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Insight into the phosphodiesterase mechanism from combined QM ⁄ MM free energy simulations pot
... to model the hydrolysis of cAMP by the enzyme PDE4B, which provides further insights on the general features of phosphate hydrolysis. The PDE superfamily of enzymes can be classified into 11 members ... Although the structure of a small frag- ment of PDE4D was reported in 1996 [5,13], key insights into the understanding of the catalytic active site of PDEs wer...
Ngày tải lên: 14/03/2014, 23:20
Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc
... mechanism of h ydrolysis of IPU and o ther GH family 49 dextranases, the a nomer configuration of the hydrolysate of IPU was determined. A polarimetric assay was carried out using panose as the substrate. ... activating the nucleophilic water m olecule) in the first step of the reaction. A carbonium ion intermediate subsequently forms, and is further attacked by the...
Ngày tải lên: 19/02/2014, 16:20
Báo cáo khoa học: Insights into the activation of brain serine racemase by the multi-PDZ domain glutamate receptor interacting protein, divalent cations and ATP ppt
... Activation of brain SR requires the presence of GAP2 together with PDZ7. Interestingly, in the absence of additional ATP, the activation induced by the presence of the PDZ4- PDZ5-PDZ6-GAP2-PDZ7-end of ... carbonyl groups. Both the geometry and the distances perfectly agree with the coordination of these metals [23]. The Fig. 4. Binding of ATP to SR change...
Ngày tải lên: 30/03/2014, 03:20
Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt
... coli agmatinase, of the loop indicated by the letter a. Fig. 5. Scheme of the binuclear manganese cluster and the localization of the side-chains of Asp153 and Asn153 in the modelled structures of wild-type ... with the same region of the arginases (Fig. 4). Since the arginase loop contains residues that interacts with the a-carboxylate group of the subst...
Ngày tải lên: 21/02/2014, 01:21
Báo cáo khoa học: Bacitracin inhibits the reductive activity of protein disulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding domain pptx
... 10 mm). The structural identity of the isolated analogs was con- firmed by MALDI-TOF ⁄ TOF MS, with a 4800 MALDI- TOF ⁄ TOF Analyzer (AB SCIEX, Framingham, MA, USA). MALDI-TOF and TOF ⁄ TOF MS spectra ... involved in the crosslink by the sin- gle-letter code. The PDI peptide side of the disulfide bond is named R1, and the bacitracin side R2. (C) MALDI-TOF MS spectrum of...
Ngày tải lên: 14/03/2014, 23:20
Báo cáo khoa học: Defining the QP-site of Escherichia coli fumarate reductase by site-directed mutagenesis, fluorescence quench titrations and EPR spectroscopy doc
... effects on the EPR properties of FrdABCD. In the case of the FrdB-T205H mutant, the [3Fe-4S] cluster line-shape is narrower than that of the wild-type (note the position of the trough in the spectrum ... Looking along the axis defi- ned by the two keto-oxygens of the prox- imal menaquinone (MQ) naphthoquinone bicycle. (B) Looking along the axis of the MQ...
Ngày tải lên: 23/03/2014, 13:20
Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt
... than the measurements of inactivation, show the same trends with respect to the effects of NaCl, CaCl 2 and EDTA as the inactivation constants. As shown by SDS ⁄ PAGE (Fig. 5), the decrease in fluorescence intensity ... [20,21]. In the present study, the first insights into the secon- dary and tertiary structure of PLD a2 from cabbage are given. The surface struc...
Ngày tải lên: 19/02/2014, 02:20
Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... as a consequence of the mutagenic replacements. Effects of the His120Asn and His145Asn mutations on the affinity of metal binding to arginase II To further examine the effects of the His120fiAsn and ... ana- lysis of the effect was not performed, the inhibition by 20 mm agmatine was eliminated by saturation with l-arginine, indicating the competitive character of...
Ngày tải lên: 20/02/2014, 02:21
Báo cáo khoa học: Investigations into the ability of an oblique a-helical template to provide the basis for design of an antimicrobial anionic amphiphilic peptide pot
... which facili- tates their interaction with the anionic membranes of microbial cells, and they exert their antimicrobial action by the use of nonreceptor-based mechanisms of Keywords anionic; ... contribute to the susceptibility of E. coli model membranes to the action of AP1. An important determinant of the susceptibility of membranes to a-AMPs is the packing chara...
Ngày tải lên: 07/03/2014, 12:20