Báo cáo khóa học: Selective release and function of one of the two FMN groups in the cytoplasmic NAD + -reducing [NiFe]-hydrogenase from Ralstonia eutropha pptx

... Selective release and function of one of the two FMN groups in the cytoplasmic NAD + -reducing [NiFe]-hydrogenase from Ralstonia eutropha Eddy van der Linden 1 , Bart W. Faber 1 , ... upon dilution. In the following we will refer to the FMN released upon reduction as FMN- a and the one located in the HoxF subunit as FMN- b. K...

Ngày tải lên: 07/03/2014, 15:20

... patterning in early embryonic development and in the antimicrobial immune response in larvae and adults. We have investigated the function of Spa ¨ tzle in a lepidopteran insect, Manduca sexta, in ... active C106 [7,13]. C106 then binds to the ectodomain of the trans- membrane receptor Toll and thereby initiates a cyto- plasmic signaling pathway, resulting in...

Ngày tải lên: 06/03/2014, 09:22

... C-terminal regions of the enzyme, and the NADPH-binding domain (residues 109–323) consisting of the central part of the polypeptide chain [8]. A small two- stranded b-sheet links the two domains. Our recent ... nonpolar parts of the protein molecule along with the peptide backbone CONH groups and the polar groups in the side chains of proteins [20,21]....

Ngày tải lên: 19/02/2014, 16:20

... in insulin regulation in the pancreas. According to the current model, binding of a putative ligand to the PAS domain disinhibits the kinase domain, lead- ing to PASKIN autophosphorylation and increased ... domain serine ⁄ threonine kinase PASKIN, or PAS kinase, links energy flux and protein synthesis in yeast, regulates glycogen synthesis and protein translation in mam...

Ngày tải lên: 06/03/2014, 00:21

... hydrolysis of 9-O-acetyl groups from sialic acids is catalysed by sialate-9-O-acetylesterase (OAE) [17,18]. In mammals two distinct forms of OAE, one in the cytoplasm and the other in the lysosomal ... found in individual bacterial strains and faecal extracts from normal individuals [4,20]. Therefore we explored the possibility that the relative levels of...

Ngày tải lên: 07/03/2014, 14:20

... of this family have similar struc- tures consisting of two domains, one that binds FMN and one that binds FAD and NADPH [8–10]. The FMN domain is homologous to flavodoxins, while the FAD and NADPH ... ADP- binding motif. This suggests the ADP-binding motif in the a1 subunit mediates the observed ATP binding, and FMN is likely situated at the interface betw...

Ngày tải lên: 20/02/2014, 01:20

... examined. Mutation of E53 to Q weakens the binding of THF ⁄ FTHF to the Gly binary complex. The results highlight the role of E53 in bind- ing of THF ⁄ FTHF and in the proper positioning of l-Ser for ... role in tetrahydrofolate ⁄ 5-formyl tetrahydro- folate binding and in the proper positioning of Cb of l-Ser for direct attack by N5 of tetrahydrofolate...

Ngày tải lên: 18/02/2014, 16:20

... J(0) and J(x N ) as a function of s , using a simple Lorentzian function. The left-hand inset shows an overview of the theoretical curve and the fitting line to highlight the two intercept points. ... related to the dis- tribution of the energy between the overall tumbling and the internal mobility, and is indicative of the degree of internal restraint...

Ngày tải lên: 18/02/2014, 16:20

... timing of steps involved in dimer formation and a ppearance of thermostabilization by phosphate has been examined. The role of the cofactor 5¢-phosphate group in the induction of stability and stabil- ization ... present from the b eginning of the r econstitution or was added at the e nd of the burst phase (5 min). The yield was the same in both exper...

Ngày tải lên: 19/02/2014, 16:20

... remit of the local ethics committee. Prior to introduction of CPOE, local standards of prescribing existed specifying the tenets of good practice, including the avoid- ance of the use of abbreviations. An ... amphoter- icin was intended. The doses of these two products are not interchangeable and the high dose prescribed would have been nephrotoxic. In the th...

Ngày tải lên: 25/10/2012, 10:39