Báo cáo khóa học: Selective release and function of one of the two FMN groups in the cytoplasmic NAD + -reducing [NiFe]-hydrogenase from Ralstonia eutropha pptx

... Selective release and function of one of the two FMN groups in the cytoplasmic NAD + -reducing [NiFe]-hydrogenase from Ralstonia eutropha Eddy van der Linden1, Bart W. Faber1, ... upondilution. In the following we will refer to the FMN released uponreduction as FMN- a and the one located in the HoxFsubunit as FMN- b.Kinetics of the release of FMN induced by reductionwith NADHWhen ... by NADH opens up the enzymesuch that the FMN- a group is released. The role of the FMN- a group in activation of the SH The H2 -NAD + activity of the enzyme after gel-ﬁltrationunder reducing...

Báo cáo khoa học: Proteolytic activation and function of the cytokine Spatzle in the innate immune response of a lepidopteran insect, Manduca sexta ppt

... patterning in earlyembryonic development and in the antimicrobial immune response in larvae and adults. We have investigated the function of Spa¨tzle in a lepidopteraninsect, Manduca sexta, in ... active C106[7,13]. C106 then binds to the ectodomain of the trans-membrane receptor Toll and thereby initiates a cyto-plasmic signaling pathway, resulting in the release of arel family transcription ... ortho-log of the product of this gene. In the clade includingSpa¨tzle-1, the branch lengths are noticeably longer and the bootstrap values are lower than in the other cladescontaining Spa¨tzle-2...

Tài liệu Báo cáo khoa học: Guanidinium chloride- and urea-induced unfolding of FprA, a mycobacterium NADPH-ferredoxin reductase Stabilization of an apo-protein by GdmCl docx

... C-terminalregions of the enzyme, and the NADPH-bindingdomain (residues 109–323) consisting of the centralpart of the polypeptide chain [8]. A small two- strandedb-sheet links the two domains. Our recent ... nonpolar parts of the protein molecule along with the peptide backbone CONH groups and the polar groups in the side chains of proteins [20,21]. Accordingto this mechanism the unfolding of FprA should ... family of glutathione reductase [8], of whichFprA is a member. The FprA monomer consists of two domains: the FAD-binding domain (residues2–108 and 324–456) consisting of the N- and C-terminalregions...

Báo cáo khoa học: Substrate preference and phosphatidylinositol monophosphate inhibition of the catalytic domain of the Per-Arnt-Sim domain kinase PASKIN ppt

... in insulin regulation in the pancreas. According to the current model, binding of a putative ligand to the PAS domain disinhibits the kinase domain, lead-ing to PASKIN autophosphorylation and increased ... domain serine ⁄ threonine kinase PASKIN, or PASkinase, links energy ﬂux and protein synthesis in yeast, regulates glycogensynthesis and protein translation in mammals, and might be involved in insulin ... PASKIN con-tains two PAS domains (PAS A and PAS B) and aserine ⁄ threonine kinase domain that might be regulated in cis by binding of so far unknown ligands to the PASdomain [7]. PASKIN shows...

Báo cáo khóa học: O-acetylation and de-O-acetylation of sialic acids in human colorectal carcinoma docx

... hydrolysis of 9-O-acetyl groups from sialicacids is catalysed by sialate-9-O-acetylesterase (OAE)[17,18]. In mammals two distinct forms of OAE, one in the cytoplasm and the other in the lysosomal ... found in individual bacterial strains and faecalextracts from normal individuals [4,20]. Therefore weexplored the possibility that the relative levels of OAT and OAEinboththeresectionmarginandcancermucosaregulate ... kit according to the manufacturers instructions. One unit of esterase activityequals 1 nmol of acetic acid released per min under the conditions used. The hydrolysis of O-acetyl groups from Neu5,9Ac2 and Neu5,7(8)9Ac3was...

Tài liệu Báo cáo khoa học: A second novel dye-linked L-proline dehydrogenase complex is present in the hyperthermophilic archaeon Pyrococcus horikoshii OT-3 pptx

... of this family have similar struc-tures consisting of two domains, one that binds FMN and one that binds FAD and NADPH [8–10]. The FMN domain is homologous to ﬂavodoxins, while the FAD and NADPH ... ADP-binding motif. This suggests the ADP-binding motif in the a1 subunit mediates the observed ATP binding, and FMN is likely situated at the interface between the a1 and b1 subunits within the ... at the interface between the a and b subunits.A comparison of the amino-acid sequences showed that the ADP-bindingmotif in the a subunit of PDH1 clearly differs from that in the a subunitof...

Tài liệu Báo cáo khoa học: Structure determination and biochemical studies on Bacillus stearothermophilus E53Q serine hydroxymethyltransferase and its complexes provide insights on function and enzyme memory doc

... examined. Mutation of E53 to Qweakens the binding of THF ⁄ FTHF to the Gly binarycomplex. The results highlight the role of E53 in bind-ing of THF ⁄ FTHF and in the proper positioning of l-Ser for ... role in tetrahydrofolate ⁄ 5-formyl tetrahydro-folate binding and in the proper positioning of Cb of l-Ser for directattack by N5 of tetrahydrofolate. Most interestingly, the structure of the complex ... observed in the case of E53QbsSHMT-Gly. The most signiﬁcant difference in the structure isobserved in the case of the bound Gly. The carboxyl-ate group of Gly is in two distinct conformations in E53QbsSHMT-Gly....

Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf

... J(0) and J(xN) as a function of s,using asimple Lorentzian function. The left-hand inset shows an overview of the theoretical curve and the ﬁtting line to highlight the two intercept points. ... related to the dis-tribution of the energy between the overall tumbling and the internal mobility, and is indicative of the degree of internal restraint of each15N–1H vectormotion. In Fig. ... suggesting the occurrence of eithera kinking or tightening of the local geometry. Asimilar discontinuity had been noted in solution also in the Antp HD [8,9] and the Antp (C39S) HD [10], and indeed,...

Tài liệu Báo cáo khoa học: Relationships between structure, function and stability for pyridoxal 5¢-phosphate-dependent starch phosphorylase from Corynebacterium callunaeas revealed by reversible cofactor dissociation studies doc

... timing of steps involved in dimer formation and a ppearance of thermostabilization byphosphate has been examined. The role of the cofactor5¢-phosphate group in the induction of stability and stabil-ization ... present from the b eginning of the r econstitution or was added at the e nd of the burst phase (5 min). The yield was the same in bothexperiments within t he experimental error. The r ecovery of activity ... formation of an a po-oligomer induced by the b inding of the nucleotide sugar. The presence of maltopentaose (5 mM) resulted in a moderate 1.5-foldincrease in the half-life of apo-CcStP.Effects of...

Báo cáo khoa học: " Medication errors: a prospective cohort study of hand-written and computerised physician order entry in the intensive care unit"

... remit of the local ethics committee. Prior tointroduction of CPOE, local standards of prescribing existedspecifying the tenets of good practice, including the avoid-ance of the use of abbreviations.An ... amphoter-icin was intended. The doses of these two products are notinterchangeable and the high dose prescribed would havebeen nephrotoxic. In the third case, vancomycin was pre-scribed 1 g intravenously ... investigate the impact of CPOE,without decision support, on MEs in the critical care setting. The data collected were viewed in terms of proportion of errors, patient outcomes arising from the...

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