Báo cáo khóa học: ICAM-1 expression is highly NF-jB-dependent in A549 cells No role for ERK and p38 MAPK docx

... ICAM-1 expression is highly NF-jB-dependent in A549 cells No role for ERK and p38 MAPK Neil S. Holden 1 , Matthew C. Catley 2 , Lisa M. Cambridge 2 , Peter J. Barnes 2 and Robert Newton 1 1 Department ... SB203580, PD098059 and U0126 on NF-jB DNA binding and NF-jB-dependent transcription To examine the role of the p38 and the ERK MAPK pathways,...

Ngày tải lên: 07/03/2014, 15:20

... Blue staining. Table 2. Quantitation and comparison of proteins in GM and WI-38 cells. From the data in Fig. 2 and others, the concentrations of Mcm proteins, ORC2 and PCNA in total protein and chromatin-bound ... ubiquitously in cancer cells than in prolif- erative cells from normal uterine cervix or cells with dysplasia. Their expression is probably more abund...

Ngày tải lên: 20/02/2014, 23:20

... were washed 4 · 20mininPBSTGandincubated in a 1 : 750 dilution of biotinylated anti-rabbit IgG (Vector Laboratories) for 1 h. Tissues were washed 4 · 20 min in PBSTG and incubated in a 1 : 200 dilution ... However, studies on insect aquaporins are lacking, and it is not known if redundancy is also present in insect tissues. The most similar proteins to AeaAQP are the D. mela...

Ngày tải lên: 20/02/2014, 23:20

... protein for recombinant expression of the a-factor receptor Ste2p itself in S. cerevisiae [25], which is the best level ever achieved for any GPCR membrane expression in yeast. Discussion In this ... for increasing the yield of properly folded recombinant human gamma interferon from inclusion bodies. J Biotechnol 52, 127– 133. Expression of olfactory receptors in yeast...

Ngày tải lên: 07/03/2014, 16:20

... hpST3dODN. This binding is increased in cells treated with IL-6 and, to a lesser extent, in cells treated with IFN-c (Fig. 6C, lanes 1 and 2). On the other hand, binding of phospho-STAT1 is detected ... sig- nalling [3–5] and of cytokines such as interleukin (IL)-6 [6]. It is also involved in transformation and tumour progression [7] and its activation, as detected i...

Ngày tải lên: 18/02/2014, 08:20

... fea- tures distinguishing easy inputs from difficult ones. 2.2 Difficulty for people and machines Before proceeding to the analysis of input difficulty in multi-document summarization, it is worth ... cover- age for difficult sets and higher coverage for easy sets. Since we are interested in identifying charac- teristics of generally difficult inputs rather than in discovering wh...

Ngày tải lên: 20/02/2014, 09:20

... interest in protein folding in vitro has expanded rapidly, mainly focusing on the production, in bacteria, of disulfide-containing proteins with poten- tial biotechnological applications. Therefore, increased attention ... an incorrect protein disulfide followed by a thiol–disulfide interchange within the substrate, leading in turn to a native disulfide. Two CXC-containing peptides, namel...

Ngày tải lên: 07/03/2014, 00:20

... suggesting that the heterologous expression of genes encoding metallo- proteins can lead to incorrect metal ion incorporation. This is observed in rubredoxin and desulforedoxins where zinc, instead ... concentration was in excess of 0.15 nmol. The reasons for this suprising drop in the radical con- centration during substrate conversion are given in the discussion. Discussion...

Ngày tải lên: 15/02/2014, 01:20

... complex interactions in living cells is increasing. Materials and methods The PTS model Here, we present the PTS model [8] in detail, list the linear conservation relations associated with it, and ... are peer-reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missin...

Ngày tải lên: 18/02/2014, 06:20

... Dynamics Limited, Newcastle, UK). Immunohistochemical staining of paraffin- embedded tissues Immunohistochemistry of normal and tumour tissues was performed according to the avidin–-biotin complex peroxi- dize ... were incubated for 30 min with preformed macromolecular complex consisting of avidin and biotinylated peroxidase (Vector Laboratories, Burlingame, CA), which retains biotin-bin...

Ngày tải lên: 19/02/2014, 00:20