Báo cáo khóa học: Identification of residues controlling transport through the yeast aquaglyceroporin Fps1 using a genetic screen ppt
Báo cáo khóa học: Identification of residues controlling transport through the yeast aquaglyceroporin Fps1 using a genetic screen ppt
... screen; glycerol; osmoregulation. The discovery of the aquaporins marked a breakthrough in our understanding of water and solute transmembrane transport [1]. Aquaporins and aquaglyceroporins [the major intrinsic ... family] have been found in archea, eubacteria, fungi, plants, animals and human [2,3]. Aqua- porins facilitate the diffusion of water across biological membrane...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Identification of tyrosine-phosphorylation sites in the nuclear membrane protein emerin pot
... Ogawa M, Kurano Y, Kawada J, Okada R, Hayashi YK, Tsukahara T & Arahata K (1996) Emerin deficiency at the nuclear membrane in patients with Emery–Dreifuss muscular dystrophy. Nat Genet 12, 254–259. 34 ... and some applica- tions. Proc Natl Acad Sci USA 76, 4350–4354. 44 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Identification of versican as an isolectin B4-binding glycoprotein from mammalian spinal cord tissue pptx
... glycoconjugate and the versican variant can be co-released from spinal cord membranes by hyaluronidase, and that the IB4-reactive glycoconjugate and the versican variant can be co-precipitated by an anti- versican ... perfectly matched with sequences of the pig versican according to data- base entry AAF19155.1 (Fig. 6). The data bank search indicated versican as the only signific...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Identification of RNase HII from psychrotrophic bacterium, Shewanella sp. SIB1 as a high-activity type RNase H pot
... S, Oda Y, Nakai T, Katayanagi K, Kitakuni E, Katsuda-Nakai C, Nakamura H, Ikehara M & Kanaya S (1992) Effect of the cavity-modulating mutations on the stability of E. coli ribonuclease HI. ... Kanaya S, Katsuda C, Kimura S, Nakai T, Kitakuni E, Nakamura H, Katayanagi K, Morikawa K & Ikehara M (1991) Stabilization of Escherichia coli ribonuclease H by introduction of an art...
Ngày tải lên: 16/03/2014, 13:20
Báo cáo khoa học: Identification of alternative promoter usage for the matrix Gla protein gene Evidence for differential expression during early development in Xenopus laevis doc
... +2852 XlMGPF7 CCG GAGCTCACTACCAAATAGAGCCTCC +1278 XlMGPF8 ATCTCAAAGTTCCTTCATAGAG +1 XlMGPF9 ATGAAGACTCTTCCAGTTATTC +3032 XlMGPF10 CCG GAGCTCGAGCCACCAAAATAACTTCTAGATCGTAAAAATTTGCC +2818 ODC-specific ... primers XlMGPR1 CACGC AAGCTTGACTTCTTGCTGTTAGAGG +3013 XlMGPR2 GGGAAGTGACTGCAACATAGAGAC +7964 Sense XlMGP-specific primers XlMGPF1 CCG GAGCTCATCAGACTGATAATCTGTG +2123 XlMGPF2 CCG GAGCTCAGCATCACTTA...
Ngày tải lên: 30/03/2014, 16:20
Tài liệu Báo cáo khoa học: Identification of critical active-site residues in angiotensin-converting enzyme-2 (ACE2) by site-directed mutagenesis docx
... data were captured using a Fuji LAS-1000 Imaging System CCD camera (aida 2.11 soft- ware for analysis). ACE2 ⁄ ACE activity assays Fluorogenic assays using the synthetic ACE2 substrate, Mca-APK(Dnp) ... course. The reaction product was quantified by using standard solutions of Mca. Acknowledgements We thank the Medical Research Council of Great Brit- ain (MRC) and the Natio...
Ngày tải lên: 20/02/2014, 01:20
Báo cáo khoa học: Identification of crucial residues for the antibacterial activity of the proline-rich peptide, pyrrhocoricin pot
... IEAKIEAVIKASEPLMQAVQAKAQQAGGEQPQQ Haemophilus ducreyi 583-615 IEAKIEAVIKASEPLMQAAQAKAQTNQAGEQQS Helicobacter pylori 578-607 AELEDKTKLLAQAAQKLGEAMANKNNAEQP Pseudomonas aeruginosa 584-615 IEAKMNALSQASTPLAQKMYAEQAQQGEDAPQ Staphylococcus ... C-terminus. For the Ala-scan of the DnaK D-E helix region, all native residues were replaced with alanine. The three native alanines, Ala591, Ala60...
Ngày tải lên: 08/03/2014, 10:20
Báo cáo khoa học: Identification of two cysteine residues involved in the binding of UDP-GalNAc to UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) ppt
... cysteine residues into either alanine or serine residues. C10 6a, 5¢-TAGAGGGGGCTA AAACAAAA-3¢; c21 2a, 5¢-ACTGTGCACTCGGCGTG AGC-3¢; c21 4a, 5¢-ACTGTGGCCTCGCAGTGAGC-3¢; c23 5a, 5¢-TAGGAGCCACCACTGTCCTC-3¢; ... other GalNAc-transferases contain different amino acids at these positions, such as DSH VEC (GalNAc-T5), DAHCEV (GalNAc-T7), DAH IEV (GalNAc-T8), DAHVEF (GalNAc-T9), and DSHCE A (ppG...
Ngày tải lên: 08/03/2014, 10:20
Báo cáo khoa học: Identification of critical residues of subunit H in its interaction with subunit E of the A-ATP synthase from Methanocaldococcus jannaschii potx
... Backbone 1 H, 13 C, and 15 N resonance assignments of subunit F of the A 1 A 0 ATP synthase from Methanosarcina mazei Go ¨ 1. Biomol NMR Assign 1, 23–25. 17 Maegawa Y, Morita H, Iyaguchi D, Yao M, Watanabe N & ... integral membrane A 0 domain, involved in ion translocation [2–4]. The primary struc- ture of the archaeal ATP synthase is similar to that of the eukaryoti...
Ngày tải lên: 30/03/2014, 04:20
Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt
... to 95 °C on a thermal heating block and cooling to 4 °C at a rate of 2 °CÆmin –1 . Name Sequence ssDNAS d(CATTCCCACCGGGACCACCAC) ssDNA L d(CATTCCCACCGGGACCACCACCATTCCCACCGGGACCACCAC) ETS-1 d(TCTCTCGGTGGCCGGGGCTCGTCGGGGTTTTGGGTCCGTCC) Htelo ... ACA G) and EWS reverse d(CGC TCG AGT CAC TAG TAG GGC CGA TCT CTG C), for pGEX–EWS; EAD forward d(CGG AAT TCA TGG CGT CCA CGG ATT ACA G) and EAD r...
Ngày tải lên: 15/02/2014, 01:20