Báo cáo khóa học: Conformational changes of b-lactoglobulin in sodium bis(2-ethylhexyl) sulfosuccinate reverse micelles A fluorescence and CD study docx

... M. Andrade et al. (Eur. J. Biochem. 271) Ó FEBS 2004 Conformational changes of b-lactoglobulin in sodium bis(2-ethylhexyl) sulfosuccinate reverse micelles A fluorescence and CD study Suzana M. Andrade, ... was used as a standard to check the apparatus response on a daily basis. Data analysis was performed by a deconvolution method using a nonlinear least...

Ngày tải lên: 07/03/2014, 15:20

... protein [1]. HN binds to sialic acid-containing receptors at the cell surface through its hemagglutinating activity (receptor-binding activity) and it also displays neuraminidase or sialidase activity ... we suggest that the binding of NDV to free gangliosides results in the formation of a conformational intermediate of HN. Materials and methods Materials Bis-ANS and octadec...

Ngày tải lên: 30/03/2014, 13:20

... double stranded oligos Oct-1, 5¢-TGT CGAATGCAAATCACTAGAA-3¢; Sp1, 5¢-ATTCGATC GGGGCGGGGCGAGC-3¢; Ets/Pea3, 5¢-GATCTCGAG CAGGAAGTTCGA-3¢; Ets (PU.1), 5¢-GGGCTGCTTG AGGAAGTATAAGAAT-3¢;Stat3,5¢-GATCCTTCTG GGAATTCCTAGATC-3¢; ... GAA AAG GGC GGG AGG TAC CGC ATC ACA GTC TC Mu-S)77/)71 GCA AAT GCG CCA GGT ACC TTC TGG GAA CTC Mu-AS)77/)71 GAG TTC CCA GAA GGT ACC TGG CGC ATT TGC Mu-S)68/)59 CCG CCC TT...

Ngày tải lên: 20/02/2014, 11:20

... HIF- 1a forward, 5¢-TCAAGCAGTAGCGAATTGGAACATTATT-3¢; HIF- 1a reverse, 5¢-TTTACACGTTTCCAAGAAAGTGATG TA-3¢; BNIP3 forward, 5¢-TTTGCTGGCCATCGGATT- 3¢; BNIP3 reverse, 5¢-ACCAAGTCAGACTCCAGTTCTT CA-3¢; aldolase ... aldolase forward, 5¢-GAATTGGATGAAAGATA AAGCCCTTA-3¢; aldolase reverse, 5¢-TTGCCAGACC ATCCGTACTG-3¢; RPL1 3A forward, 5¢-CTCAAGGTC GTGCGTCTGAA,-3¢: RPL13 reverse, 5¢-TGGCTGTCAC TG...

Ngày tải lên: 16/03/2014, 05:20

... required, after replicon initiation had taken place. Immunofluorescence staining of total cellular and chromatin-bound PCNA T24 were grown and incubated on coverslips. Two sets of hypoxic cells and of ... During the last wash total DNA was stained with bisbenzimide (2 lgÆmL )1 in NaCl/ P i ). Finally PCNA (Alexa FluorÒ 568 stain) and total DNA (bisbenzimide stain) were visualiz...

Ngày tải lên: 21/02/2014, 00:20

... (steady- state, time-resolved and ANS binding), and anisotropy were used to probe conformational features of LamA before and after heat or chemical treatment. It was suggested that upon heating at ... [6,7]. Even in the absence of structural data, valuable information about the local and global dynamics of LamA can be inferred from inspection of the fluorescence decays...

Ngày tải lên: 07/03/2014, 21:20

... structure of 17b-HSDcl of increasing NaCl concentrations at pH 2.0 and 25 °C are shown in Fig. 3A, and these indicate that an increase in NaCl concentration induces an increase in b S structure. At 300 ... an alkaline pH of 13.0, there was an alteration in the shape and intensity of Fig. 1. Far- and near-UV CD spectra of 17b-HSDcl at different pH values. (A) Far-UV...

Ngày tải lên: 16/03/2014, 13:20

... While maintain- ing the ATPase domain of DnaK, different regions of the more divergent substrate-binding domain were exchanged: (a) a- helical subdomain and C-terminal residues in KKCC; and (b) ... times, and (d) after 40 min, the 44 kDa band was the most abundant. In the absence of nucleotides or in the presence of ADP, KKCC and KCCC generated mainly 46 and 44 kDa fr...

Ngày tải lên: 16/03/2014, 22:20

... conformation and dynamic that is maintained in both N- and O-like states. The exact nature of the alteration in dynamic and how it is linked to the small observed changes of orientation of helix ... 163 and 209, with only 68 and 163 located in loops and all others located in helices A, B, D, E and G. Application of this label- ling scheme in combination with...

Ngày tải lên: 23/03/2014, 13:20

... globin expressed in the brain. Nature 407, 520–523. 6 Kawada N, Kristensen DB, Asahina K, Nakatani K, Minamiyama Y, Seki S & Yoshizato K (2001) Charac- terization of a stellate cell activation-associated ... with an alanine in Mb (MbF-P8 8A) , and of Ala90 (AlaF2) with a proline in Ngb (NgbF -A9 0P) (see Fig. 1). Fig- ures 4A, B depict the spectra obtained for NgbF-wt at n...

Ngày tải lên: 30/03/2014, 01:20