Báo cáo khoa học: Identification of yeast aspartyl aminopeptidase gene by purifying and characterizing its product from yeast cells potx

... Identification of yeast aspartyl aminopeptidase gene by purifying and characterizing its product from yeast cells Ryo Yokoyama*, Hiroshi Kawasaki and Hisashi Hirano Supramolecular ... The amount of peptide generated was estimated from the ratio of the height of the product peptide peak versus the sum of the heights of the product peptide and su...

Ngày tải lên: 07/03/2014, 12:20

... 684.5 from 100 fmol of synthetic SQA-NPFF (A), at m/z 684.5 from HPLC fractions of NPFF-IR peak 2 (B), at m/z 990.0 from 100 fmol of synthetic hNPAF (D) and at m/z 990.0 from HPLC fractions of NPFF-IR peak3(E)andMS/MSspectraofthe[M+2H] 2+ precursor ... to explain the generation of NPFF: NPFF could be generated by processing of the hproNPFF A by enzymes recognizing a...

Ngày tải lên: 31/03/2014, 07:20

... shock, cells were pregrown in the presence of 0.8 M NaCl and shifted to medium without salt. Growth was monitored as above. Mutagenesis and screening Random mutations in FPS1 were introduced by transform- ing ... reported here regarding Gly348 and His350 are likely to be of more general relevance for the understanding of the structure-function relationship of the MIP family...

Ngày tải lên: 07/03/2014, 15:20

... Affinity of RGG3 for binding to a G-quadru- plex DNA and RNA. Fig. S2. CD spectra of DNAs and RNAs. Fig. S3. Binding affinity of EWS and RBD to Htelo. Fig. S4. In vitro arginine methylation of RGG3 by PRMT3. Fig. ... (lanes 2 and 4) and 32 P-labeled Htelo (lanes 1 and 2) or rHtelo (lanes 3 and 4). (B) EMSA was performed with RGG3 (lanes 2 and 4) and 32 P-labeled rHte...

Ngày tải lên: 15/02/2014, 01:20

... and structural analysis of OSs in LOSs of lpxX and lpxL mutants The OS portion of each LOS was obtained after mild acid hydrolysis and analyzed for its glycosyl composi- tions and by MALDI-TOF ... features of both mutants was performed to study the functions of these genes and the structures of their resultant LOSs in vitro and in vivo. Results Identification of...

Ngày tải lên: 18/02/2014, 14:20

... adjustments and lead to a better understanding of organismal responses to environmen- tal change. Gene expression datasets can be clustered into groups of genes that represent different compart- ments of ... Expression of most transcripts (mainly heat shock proteins and genes involved in cellular homeostasis) showed a high and rapid increase at 3–7 days of exposure, followed...

Ngày tải lên: 18/02/2014, 16:20

... found to have a molecular mass of 64 kDa, and to contain two tandem repeats and a Glu-rich region. The structure of the protein and that of its DNA are similar to those of starmaker, a protein involved in ... available online: Fig. S1. Sequences of the omm-64 cDNA and its deduced protein. Fig. S2. Alignment of amino acid sequences of OMM- 64 and starmaker. Fig. S3....

Ngày tải lên: 18/02/2014, 17:20

... first identification of triterpene hydroxylase cDNA from any plant species. Successful identification of a b-amyrin and sophoradiol 24-hydroxy- lase from the inducible family of cytochrome P450 genes ... Werck-Reich- hart D & Bak S (2004) Comparative genomics of rice and Arabidopsis. Analysis of 727 cytochrome P450 genes and pseudogenes from a monocot and a dicot....

Ngày tải lên: 19/02/2014, 07:20

... down-regulation of a substantially larger subset of genes. Identification and characterization of a subset of ISGF3-independent STAT2-dependent target genes To identify those genes whose expression ... Comparable transcriptional activation of the PKR, 2¢5¢OAS and Mx genes was observed in IFN-stimulated U6A-2 and U6A-2VV-II cells, and the promoters of all three genes cont...

Ngày tải lên: 19/02/2014, 07:20

... Tsubota and Y. Kariya for helpful suggestions and discussions. This work was supported by Grants-in-Aid from the Minis- try of Education, Culture, Sports, Science and Tech- nology and from the ... clearly showed doublet bands at approximately 75 kDa and two minor bands at 95 and 110 kDa. The conditioned media of HSC-4 and HEK293 cells also showed weak immunoreactive...

Ngày tải lên: 19/02/2014, 07:20