Báo cáo khoa học: Identiﬁcation of yeast aspartyl aminopeptidase gene by purifying and characterizing its product from yeast cells potx

... Identiﬁcation of yeast aspartyl aminopeptidase gene by purifying and characterizing its product from yeast cells Ryo Yokoyama*, Hiroshi Kawasaki and Hisashi HiranoSupramolecular ... Theamount of peptide generated was estimated from theratio of the height of the product peptide peak versusthe sum of the heights of the product peptide and sub-strate peaks.Puriﬁcation of yeast aspartyl ... FEBSResultsPuriﬁcation of yeast aspartyl aminopeptidase A novel aspartyl aminopeptidase was puriﬁed from yeast cells by ultracentrifugation, ammonium sulfatefractionation, and chromatography. Mono...

Báo cáo khoa học: Identiﬁcation of proNeuropeptide FFA peptides processed in neuronal and non-neuronal cells and in nervous tissue potx

... 684.5 from 100 fmol of synthetic SQA-NPFF (A), at m/z 684.5 from HPLC fractions of NPFF-IR peak 2 (B), at m/z 990.0 from 100 fmol of synthetic hNPAF (D) and at m/z 990.0 from HPLC fractions of NPFF-IRpeak3(E)andMS/MSspectraofthe[M+2H]2+precursor ... toexplain the generation of NPFF: NPFF could be generated by processing of the hproNPFFA by enzymes recognizinga nonbasic motif. This hypothesis is supported by thepresence of a conserved ... isolated from cell extracts wascompared with that observed in mouse spinal cord.NPFF-related peptides from cells and tissues were extracted and puriﬁed by RP-HPLC, assessed by radioimmunoassayand...

Báo cáo khóa học: Identiﬁcation of residues controlling transport through the yeast aquaglyceroporin Fps1 using a genetic screen ppt

... shock, cells were pregrown in the presence of 0.8MNaCl and shifted to medium without salt. Growth wasmonitored as above.Mutagenesis and screeningRandom mutations in FPS1 were introduced by transform-ing ... reported here regarding Gly348 and His350 arelikely to be of more general relevance for the understanding of the structure-function relationship of the MIP family of channel proteins.AcknowledgementsThis ... concentrationgradient, cells were grown in liquid YNB medium to a D600 of approximately 0.7. Cells were harvested, washed and suspended in ice-cold Mes buffer (10 mMMes, pH 6.0) to adensity of 40–60 mg cells ml)1....

Tài liệu Báo cáo khoa học: Identiﬁcation of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt

... Afﬁnity of RGG3 for binding to a G-quadru-plex DNA and RNA.Fig. S2. CD spectra of DNAs and RNAs.Fig. S3. Binding afﬁnity of EWS and RBD toHtelo.Fig. S4. In vitro arginine methylation of RGG3 by PRMT3.Fig. ... (lanes 2 and 4) and 32P-labeled Htelo (lanes 1 and 2) orrHtelo (lanes 3 and 4). (B) EMSA was performed with RGG3 (lanes2 and 4) and 32P-labeled rHtelo (lanes 1 and 2) or mut rHtelo (lanes3 and ... presence of various amounts of RGG3.The CD spectrum of Htelo, a hybrid (3 + 1) form,showed a strong positive band at 290 nm and a nega-tive band at around 235 nm, whereas the addition of 1ratio...

Tài liệu Báo cáo khoa học: Identiﬁcation of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc

... and structural analysis of OSs inLOSs of lpxX and lpxL mutantsThe OS portion of each LOS was obtained after mildacid hydrolysis and analyzed for its glycosyl composi-tions and by MALDI-TOF ... features of both mutants wasperformed to study the functions of these genes and the structures of their resultant LOSs in vitro and in vivo.Results Identiﬁcation of putative late acyltransferasegenes ... predicted gene product of 307 or 325 amino acids (Fig. 1). Upstreamsequence analysis of lpxX revealed the presence of a gene encoding aspartyl- tRNA synthetase (ats), and sequence analysis of the...

Tài liệu Báo cáo khoa học: Identiﬁcation of differentially expressed genes of the Paciﬁc oyster Crassostrea gigas exposed to prolonged thermal stress docx

... adjustments and lead to a betterunderstanding of organismal responses to environmen-tal change. Gene expression datasets can be clusteredinto groups of genes that represent different compart-ments of ... Expression of most transcripts(mainly heat shock proteins and genes involved in cellular homeostasis)showed a high and rapid increase at 3–7 days of exposure, followed by adecrease at 14 days, and ... differentialexpression of a number of genes that encode elements of the transcription and translation machinery, includ-ing transcription factors, ribosomal proteins and elon-gation factors. After 24 days of exposure...

Tài liệu Báo cáo khoa học: Identiﬁcation of a novel matrix protein contained in a protein aggregate associated with collagen in ﬁsh otoliths pdf

... found to have a molecular mass of 64 kDa, and to containtwo tandem repeats and a Glu-rich region. The structure of the protein and that of its DNA are similar to those of starmaker, a protein involvedin ... availableonline:Fig. S1. Sequences of the omm-64 cDNA and its deduced protein.Fig. S2. Alignment of amino acid sequences of OMM-64 and starmaker.Fig. S3. Northern hybridization analysis of omm-64transcripts.Fig. ... observed by gel elec-trophoresis. These substances may be aggregates of proteins and polysaccharides, and may play importantroles in formation of the phases and ⁄ or morphologies of the crystals...

Tài liệu Báo cáo khoa học: Identiﬁcation of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx

... ﬁrst identiﬁcation of triterpene hydroxylase cDNA from any plantspecies. Successful identiﬁcation of a b-amyrin and sophoradiol 24-hydroxy-lase from the inducible family of cytochrome P450 genes ... Werck-Reich-hart D & Bak S (2004) Comparative genomics of rice and Arabidopsis. Analysis of 727 cytochrome P450 genes and pseudogenes from a monocot and a dicot. PlantPhysiol 135, 756–772.29 D’Auria ... for 24 h. Then, the cells were collected and suspen-ded in 25 mL of 40% potassium hydroxide and 25 mL of methanol and reﬂuxed for 2 h. Products were extractedwith 50 mL of hexane. The hexane...

Tài liệu Báo cáo khoa học: Identiﬁcation of GAS-dependent interferon-sensitive target genes whose transcription is STAT2-dependent but ISGF3-independent doc

... down-regulation of a substantially larger subset of genes. Identiﬁcation and characterization of a subset of ISGF3-independent STAT2-dependent targetgenesTo identify those genes whose expression ... Comparabletranscriptional activation of the PKR, 2¢5¢OAS and Mxgenes was observed in IFN-stimulated U6A-2 and U6A-2VV-II cells, and the promoters of all three genescontained the expected ISRE ... expression of these genes: TLR3,RBMS3. Closer examination of the IFN-inducibleexpression of the TLR3 gene using quantitative PCRconﬁrmed its diminished expression in U6A and U6A-2-VV-II cells...

Tài liệu Báo cáo khoa học: Identiﬁcation of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomodulin/mac25) doc

... Tsubota and Y. Kariya for helpful suggestions and discussions. Thiswork was supported by Grants-in-Aid from the Minis-try of Education, Culture, Sports, Science and Tech-nology and from the ... clearly showed doublet bands at approximately75 kDa and two minor bands at 95 and 110 kDa. Theconditioned media of HSC-4 and HEK293 cells alsoshowed weak immunoreactive bands at the same posi-tions. ... Higashi S,Kitamura H & Miyazaki K (2005) Production of soluble matriptase by human cancer cell lines and cellsurface activation of its zymogen by trypsin. J CellBiochem 95, 632–647.33 Takeuchi...

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