Báo cáo khoa học: Computer-assisted mass spectrometric analysis of naturally occurring and artificially introduced cross-links in proteins and protein complexes potx

... compilation ª 2005 FEBS 291 Computer-assisted mass spectrometric analysis of naturally occurring and artificially introduced cross-links in proteins and protein complexes Leo J. de Koning 1 , Piotr T. Kasper 1 , ... structures, chemical modifications and cross-linking in protein mixtures, complexes, and assemblies. Proteins A single protein, a list o...

Ngày tải lên: 07/03/2014, 12:20

... transcription–PCR analysis of unknown serial analysis of gene expression tags; rSAGE, reverse serial analysis of gene expression; SAGE, serial analysis of gene expression; TSAT-PCR, two-step analysis of unknown ... sequence downstream of the most 3¢ NlaIII restriction site. In contrast, the remaining PCR product was a large size band of the no. 10 product, in which...

Ngày tải lên: 07/03/2014, 04:20

... determined from data obtained by changing the concentration of the linking intermediate and measuring the corresponding rates of the modules (i.e. from module rates versus S data). Changes in the intermediate ... effect of removing the inhibition of AKI and AKII, and the inhibition of HSDHI and HSDHII, separately. In both procedures, the corresponding maximal rates were...

Ngày tải lên: 14/03/2014, 23:20

... blockage of the ATP-binding site in the catalytic domain of the kinase. As the majority of kinase inhibitors that are discovered by high- throughput screening methods target the ATP-binding pocket of ... of 100 lm) and will contain the amount of protein contained in a single cell. Spotting is performed using a noncontact piezo-electric spotter (inkjet tech- nology), with a...

Ngày tải lên: 16/03/2014, 00:20

... derived inductively. Before the analysis begins, an initial set of functions breaks each sentence of a paragraph into strings of words, and, in certain circumstances, reforms discontinuous ... ING), (AND WITH IT THE TRAVELING PUBLIC'S HABITS). Now consider the analysis of a text contain- ing this sentence, and so of the paragraph frame contain- ing a message-pai...

Ngày tải lên: 23/03/2014, 13:20

... literature. In one of these, 40% increase in the activity of the supply module results in less than 4% increase in flux, while in nitesimal MMCA predicts more than 30% increase in flux. In addition, it ... to increase the flux by manipulating the activity of demand. The impossibility of increasing the flux by changing the activity of a single module is due to an abrupt decr...

Ngày tải lên: 30/03/2014, 10:20

... concentration of inhib- itor, and n is the number of inhibitor binding sites on the protein [36,37]. Structural models of the complex Molecular docking of the 8-kDa domain of pol b and SQMG was ... the interaction interfaces of the SQG and fatty acid separately. We then combined these two interaction interfaces and identified the SQMG interaction interface of the 8-kDa...

Ngày tải lên: 30/03/2014, 20:20

... reports of emerging multidrug resistant strains [3]. Pathogenic Yersiniae are typically taken up through ingestion and first reach the intestine. The Yersinia surface protein invasin binds to b1 integrins ... cell line was maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum, 1% glutamine and 100 lgÆmL )1 streptomycin and penicillin in a humidi...

Ngày tải lên: 16/02/2014, 15:20

... (1993) Evaluation of secondary structure of proteins from UV circular dichroism spectra using an unsupervised learning neural network. Protein Eng 6, 383–390. 27 Fersht A (1999) Structure and Mechanism in Protein Science: ... being dominant. The fluorescence and near-UV CD spectra point to tight packing of the aromatic residues in the core of the protein. From the near-U...

Ngày tải lên: 19/02/2014, 02:20

... hydrolysis function of the protein may be related to some critical steps in the conversion of inactive forms of protein to that of active forms. It is interesting and intriguing to note that in the absence of ... (1) without OsDmc1 deproteinized after 15 min; (2) with 5.0 l M OsDmc1 protein without deproteinization; and (3) with 5.0 lM OsDmc1 protein with deproteiniz...

Ngày tải lên: 19/02/2014, 07:20