Báo cáo khoa học: Computer-assisted mass spectrometric analysis of naturally occurring and artiﬁcially introduced cross-links in proteins and protein complexes potx

... compilation ª 2005 FEBS 291 Computer-assisted mass spectrometric analysis of naturally occurring and artiﬁcially introduced cross-links in proteins and protein complexes Leo J. de Koning1, Piotr T. Kasper1, ... structures, chemical modiﬁcations and cross-linking in protein mixtures, complexes, and assemblies. Proteins A single protein, a list of proteins from a mixture orfrom a protein complex or a complete ... general, rapid and relativelysimple procedures for the analysis of naturally occur-ring cross-links, e.g. disulﬁde bonds, and artiﬁcially introduced cross-links in proteins. Cross-links imposedistance...

Báo cáo khoa học: Semi-nested PCR analysis of unknown tags on serial analysis of gene expression potx

... transcription–PCR analysis of unknown serial analysis of geneexpression tags; rSAGE, reverse serial analysis of gene expression; SAGE, serial analysis of gene expression; TSAT-PCR, two-step analysis of unknown ... sequence downstream of the most 3¢NlaIII restriction site. In contrast, the remaining PCRproduct was a large size band of the no. 10 product, in which sequences of UP-II and oligo(dT16) were ... reaching theend of an RNA template, it added three to ﬁve resi-dues (predominantly dC) to the 3¢-end of the ﬁrst-strand cDNA. The 5¢-cap oligonucleotide contained aterminal stretch of G residues...

Báo cáo khoa học: Modular metabolic control analysis of large responses in branched systems – application to aspartate metabolism potx

... determined from data obtained bychanging the concentration of the linking intermediate and measuring the corresponding rates of the modules(i.e. from module rates versus S data). Changes in theintermediate ... effect of removing the inhibition of AKI and AKII, and theinhibition of HSDHI and HSDHII, separately. In bothprocedures, the corresponding maximal rates wereadjusted such that the starting values ... v1arefairly constant, in most of the range of r1, and showreasonably high values. However, the range of r1values, maintaining the concentration of the linkingintermediate at plausible...

Báo cáo khoa học: Antibody-based proteomics Analysis of signaling networks using reverse protein arrays pdf

... blockage of the ATP-binding site in the catalytic domain of the kinase. As the majority of kinase inhibitors that are discovered by high-throughput screening methods target the ATP-bindingpocket of ... of 100 lm) and will contain the amount of protein contained in a single cell. Spotting is performedusing a noncontact piezo-electric spotter (inkjet tech-nology), with a spotting capacity of ... the kinase inhibitor in ques-tion is immobilized and used as ‘bait’ to ﬁsh forinteracting proteins in cell or tissue lysates [19].Alternatively, tracing the functional effects of compounds in...

Báo cáo khoa học: "On-Line Semantic Analysis of English Texts" ppt

... derived inductively. Before the analysis begins, an initial set of functions breaks each sentence of a paragraph into strings of words, and, in certain circumstances, reforms discontinuous ... ING), (AND WITH IT THE TRAVELING PUBLIC'S HABITS). Now consider the analysis of a text contain- ing this sentence, and so of the paragraph frame contain- ing a message-pair for each of ... damp." In British English that sentence allows two quite different interpretations: "a certain kind of human being is in a certain state," and "a certain kind of chemical...

Báo cáo khoa học: Modular metabolic control analysis of large responses The general case for two modules and one linking intermediate docx

... literature. In one of these, 40% increase in theactivity of the supply module results in less than 4% increase in ﬂux, while in nitesimal MMCA predicts more than 30% increase in ﬂux. In addition,it ... to increase the ﬂux by manipulating the activity of demand. The impossibility of increasing the ﬂux by changing the activity of a single module is due to an abrupt decrease of the control of ... inside the supply module and measure thechanges in the intermediate and the demand rate and, second, to perturb the demand module and measurethe changes in the intermediate and the supply rate.This...

Báo cáo khoa học: Sulfoquinovosylmonoacylglycerol inhibitory mode analysis of rat DNA polymerase b pdf

... concentration of inhib-itor, and n is the number of inhibitor binding sites on the protein [36,37].Structural models of the complexMolecular docking of the 8-kDa domain of pol b and SQMG was ... the interaction interfaces of theSQG and fatty acid separately. We then combinedthese two interaction interfaces and identiﬁed theSQMG interaction interface of the 8-kDa domain. Analysis of ... nuc-leotide, single-strand and singly primed), and 0.15 nmol of the full-length pol b or the 8-kDa domain of pol b. Variousconcentrations of inhibitors were added to the bindingmixture, followed by incubation...

Tài liệu Báo cáo khoa học: Post-translational modiﬁcation of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion pptx

... reports of emerging multidrug resistant strains [3]. PathogenicYersiniae are typically taken up through ingestion and ﬁrst reach the intestine. The Yersinia surface protein invasin binds to b1 integrins ... cell line was maintained in Dulbecco’smodiﬁed Eagle’s medium (DMEM) containing 10% fetalbovine serum, 1% glutamine and 100 lgÆmL)1streptomycin and penicillin in a humidiﬁed atmosphere of 5% ... In addition to actin, other host cell proteins have beenshown to bind to the virulence factor YpkA, includingOTUB1 [13]. In order to investigate the role of OTUB1 in Yersinia invasion of HEK293T cells,...

Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt

... (1993)Evaluation of secondary structure of proteins fromUV circular dichroism spectra using an unsupervisedlearning neural network. Protein Eng 6, 383–390.27 Fersht A (1999) Structure and Mechanism in Protein Science: ... being dominant.The ﬂuorescence and near-UV CD spectra point to tight packing of thearomatic residues in the core of the protein. From the near-UV CD signals and activity data as a function of ... present in all members of the PLD superfamily. However, there is low similar-ity in the remaining parts of the molecules. Most mam-malian PLDs contain a PX and a PH domain instead of the C2 domain...

Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by ﬂuorescence resonance energy transfer and the role of ATP hydrolysis pptx

... hydrolysisfunction of the protein may be related to some criticalsteps in the conversion of inactive forms of protein tothat of active forms. It is interesting and intriguing tonote that in the absence of ... (1)without OsDmc1 deproteinized after 15 min; (2) with 5.0 lMOsDmc1 protein without deproteinization; and (3) with 5.0 lMOsDmc1 protein with deproteinization after 15 min as indicated in ﬁgure with ... latter. Interestingly, it appears that protein binding to ssDNA in the absence of any nucleotidecofactor results in attenuation of spontaneous anneal-ing (compare line 1, Fig. 3 with line 2,...

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