Báo cáo khoa học: The natural mutation by deletion of Lys9 in the thrombin A-chain affects the pKa value of catalytic residues, the overall enzyme’s stability and conformational transitions linked to Na+ binding pdf

... domain [8]. These domains have been shown to interact with other molecules, including DNA [10,11] and proteins [1] and are responsible for mediat- ing Evi1 inhibition of apoptosis. Interactions of ... activity, including: two dis- tinct zinc finger motifs of seven and three repeating units at the N-terminus and towards the C-terminus, respectively [8]; a central repressor d...

Ngày tải lên: 29/03/2014, 08:20

... 169 The natural mutation by deletion of Lys9 in the thrombin A-chain affects the pK a value of catalytic residues, the overall enzyme’s stability and conformational transitions linked to Na + binding Raimondo ... sodium binding. X-ray structures of human thrombin show that the A-chain closely follows the contour of the cat...

Ngày tải lên: 07/03/2014, 12:20

... FEBS bean and soybean. When binding of arginine in the presence of tRNA was investigated, some changes in the binding architecture were observed [35], in that N(153:Y), in addition to interacting with ... N of the guanidine group that is replaced by the heteroatom in l-thioarginine, locking the guanidino nitrogens into the arginine-like tauto- meric form....

Ngày tải lên: 18/02/2014, 13:20

... into training and test subsets with the ratio of 9:1 we randomly split the data into training, validation and test sets with the ratio of 8:1:1. We then run our experiments and measured contingency ... test set and the corre- sponding 9 remaining sets were merged to a train- ing set. We kept the labels of the training set to determine model parameters through max...

Ngày tải lên: 20/02/2014, 04:20

... requires the assumption that the referent of the urn of coffee is in the other room. Schematically, one could represent this kind of inference as in Fig. 2 -/~ is the goal, ~ the instruction to ... with coffee. Notice that, in the same way that the generatee affects the execution of the generator, so the enabled action affects the execution of...

Ngày tải lên: 20/02/2014, 21:20

... as a standard. The amounts of chromatophores and standard protein in the different lanes of a single gel were kept in the linear range of the luminol assay response. Light-induced ATP synthesis Light-driven ... view of (A), showing only the data obtained in the absence of Du.(C)Theratioof the best-fitting functions and of the data points of wild-type ove...

Ngày tải lên: 21/02/2014, 03:20

... facilitating the passage of choline (Fig. 2B). In the case of the Glu mutation, the b value is linked to the k 2 value and thus both cannot be estimated independently. However, if b is set to 1 (i.e. ... inhibition of propidium [21] and the increase of remaining activity upon peripheral site saturation by fasciculin [24]. Finally, minor deviations of p...

Ngày tải lên: 07/03/2014, 05:20

... upgraded by a further 3 per cent by retagging problematic strings of words prior to disambiguation and by altering the probability weightings for sequences of three tags. The remaining 3 to ~ ... potential tag B divided by the frequency of A. Subsequently, it was changed to the frequency of A followed by B divided by the product of the frequency...

Ngày tải lên: 08/03/2014, 18:20

Ngày tải lên: 08/03/2014, 18:20

... sequence of words. The distance of two surface patterns is defined as the fraction of the LD value to the length of the longer sequence. For estimating the number of clusters k, we ap- ply the stability- based ... structure of text. Then the system is trained to identify entity pairs that assume a relation and to classify them into pre-defined relations....

Ngày tải lên: 23/03/2014, 16:21