Báo cáo khoa học: Dissociation/association properties of a dodecameric cyclomaltodextrinase Effects of pH and salt concentration on the oligomeric state pot

... general transcriptional co-activators that contain histone and transcription factor acetylation activities [30]. In addi- tion, CBP contains a number of protein-binding domains that mediate transcription ... catalyze mono-, di- and trimethyla- tion of H3K4, regulation of which can have distinct functional consequences. MLL1 contains a number of conserved functional domains tha...

Ngày tải lên: 16/02/2014, 14:20

... further study the mechanism of action of bacitra- cin, the reduction of insulin was analyzed in the pres- ence of the PDI a domain and DsbA, which are capable of catalyzing the oxidation and ... domains, a, b, b¢, and a , a linker region x between the b¢ and a domains, and a C-terminal acidic extension. The a and a domains contain the CGHC act...

Ngày tải lên: 16/02/2014, 14:20

... ‘oblig- atory auxiliary flip’. (18) (dass) (that) Maria Maria einen a Mann man hat has lieben love k ¨ onnen can (that) Maria was able to love a man (19) (dass) Maria hat einen Mann lieben k ¨ onnen (20) * (dass) ... infinitival inflec- tion, yet acts as a complement of the perfectizer haben, which syntactically requires a past partici- ple. Only modals, AcI-verbs such as sehen and...

Ngày tải lên: 20/02/2014, 18:20

... (LDA).Thisstatistical multivariate method is supervised. It searches for the variables containing the greatest interclass variance and the smallest intraclass variance, and constructs a linear combination ... greatest part of the original data information. In the second part of our work, we focused on the biochemical information available i n infrared s pectra, and w...

Ngày tải lên: 21/02/2014, 03:20

... 5¢-CAGCCGCCGTAGAA GTT GTAAGTCATCGCAAAG-3¢,5¢-CGCCGCCACCAGGG AA CATCCAGTCAATATCTAC-3, and 5¢-GCCGCCAC CAGGGAA TTGCCAGTCAATATCTAC-3¢, respectively. Confirmation of the mutated nucleotides by automated sequencing was carried ... In the case of the E315Q mutant, an additional faint band was also seen at an M r of  43 000. This band appeared as a degradation product during freezing...

Ngày tải lên: 20/02/2014, 01:20

... basal level of sumoylation was greatly enhanced by cotransfection of PIASy, an E3 ligase for SUMO-1. The functional consequence of abolishing sumoylation was enhanced activation both of a transiently ... principal sumoylation site and K503 a secondary one. Both sites are important for transcriptional activity and their mutation causes a large enhancement of Myb-dependent...

Ngày tải lên: 21/02/2014, 00:20

... tail separation was in accordance with both the ET and hydrodynamic data of primed a IIb b 3 . Our revised mod- els further support an alternative view of the confor- mational states and mechanism ... thermodynamic cost of such a huge conformational change could still be manageable, its physiological need is unclear, and alternative explana- tions for the role of the...

Ngày tải lên: 18/02/2014, 04:20

... localized on the backbone, with little accompa- nying cleavage of weaker side-chain modifications such as glycosylated [37] and phosphorylated structures [38] (and even of noncovalent bonding and conformational tertiary ... mod- ifications [42]. Fig. 9. ECD spectral data from the RNase A deamidation samples of Fig. 8. Deamidation at an individual residue of a specific pro...

Ngày tải lên: 18/02/2014, 16:20

... experiments and theoretical analyses suggest that membrane association and plasma membrane targeting of K-Ras are a conse- quence of the electronegative sensing function of the C-terminal domain of this ... Biophys Acta 401, 509–529. 42 Jacobson K & Papahadjopoulos D (1975) Phase transi- tions and phase separations in phospholipid membranes induced by changes in temper...

Ngày tải lên: 19/02/2014, 00:20

... product concentration, v z and v s are the initial and steady -state velocities, respectively, and k is the pseudo-first order rate constant describing the presteady state of the reaction. Based on ... concentration and k ass and k diss are the associ- ation and dissociation rate constants, respectively. k was determined for four to six inhibitor concentrations....

Ngày tải lên: 19/02/2014, 17:20