Báo cáo khoa học: Evolutionary origin and divergence of PQRFamide peptides and LPXRFamide peptides in the RFamide peptide family pdf

... 2006 The Authors Journal compilation ª 2006 FEBS 1743 Evolutionary origin and divergence of PQRFamide peptides and LPXRFamide peptides in the RFamide peptide family Insights from novel lamprey RFamide ... similarity to PQRFamide peptides. Although the C-terminal region of lamprey PQRFamide peptides also showed high similarity to LPXRFamide peptides, the N-terminal region of lamprey PQRFamide peptides showed ... gene. To clarify the evolutionary origin and divergence of PQRFamide and LPXRFamide peptides, we sought to identify novel RFamide peptides from the brains of sea lamprey Petromyzon marinus. Lam-preys...

Tài liệu Báo cáo khoa học: Interactions between M proteins of Streptococcus pyogenes and glycosaminoglycans promote bacterial adhesion to host cells pdf

... protein H appear to be involved in the interaction as the binding of 125I-labelled DS was reduced to isogenicderivatives of the AP1 strain lacking either of these surfaceproteins (Fig. 2). The ... IgGFc-binding to the S-domain, and albumin-binding to the C-repeats [31].Recombinantly expressed fragments rA–S and rS–C3 are indicated. (D) The M1 protein and the A–S and S–C3 fragments of M1 ... charged sulfate groups of the GAGchains and positively charged regions of the ligand. Typi-cally, the heparin-binding domains of known GAG-bindingproteins are rich in basic amino acids that are...

Báo cáo khoa học: Human anionic trypsinogen Properties of autocatalytic activation and degradation and implications in pancreatic diseases potx

... existed in the presence of salts or other proteins, as the routinely used in vitrobiochemical system obviously lacked the variety of salts and proteins present in the intra-acinar environment or in pancreatic ... mixturescontained only active cationic trypsin by the end of the 60 min incubation (Fig. 11A). Both the single-chain form and the double-chain form (denoted by bands A and B in Fig. 11) were observed. In ... double-chaintrypsin results in the appearance of band B, whichcorresponds to the N-terminal chain of double-chaintrypsin. For a more detailed description of the uniqueproperties of double-chain...

Báo cáo khoa học: "Diagram Understanding Using Integration of Layout Information and Textual Information" ppt

... Figure 3: The shape of leaf 2 Diagram Understanding 2.1 PBF Diagram Understanding A diagram in the PBF consists of its title and ele- ments. A title shows the subject of the diagram and it ... as shown in Figure 1 and 2. Taking advantage of this, we propose here a new method for analyzing the PBF diagrams by using natural language information. In the PBF, many kinds of information ... sketches in the diagram. Step 2. describe the following kinds of information for each word in the diagram. 1. ID number 2. writing 3. ID number of the corresponding element 4. type of correspondence...

Báo cáo khoa học: "High-precision Identiﬁcation of Discourse New and Unique Noun Phrases" docx

... having speciﬁc syntactic structure,2. appearing in the ﬁrst sentence of some text in the training corpus,3. exhibiting the same pattern as several expres-sions of type (2),4. appearing in the ... cross-validationon these 19 texts in order to ﬁnd the settings with the best precision for the class. Thesesettings are then used to train Ripper on all the 19ﬁles and test on the reserved one. The whole ... currently of 32 features.They can be divided into three groups:1. Syntactic Features. We encode part of speech of the head word and type of the determiner.Several features contain information on the characters,...

Báo cáo khoa học: An inserted loop region of stromal ascorbate peroxidase is involved in its hydrogen peroxide-mediated inactivation pot

... chi-meric APXs in the elution buffer, the wavelength of the Soret peak ranged from 405 to 407 nm, lying in the range between those of the APX-B and the stro-mal APX of spinach. On the basis of these ... monodehydroascorbateradicals.There are two APX isoforms in chloroplasts, one of which is soluble in the stroma and the other of whichis bound to the stromal side of thylakoid membranes.Both isoforms are involved in the ... auto-oxidation of the remaining ascorbate, reactswith APX [5] and leads to inactivation of the stro-mal APX. This property has the advantage of allow-ing a precise comparison of the susceptibility of various...

Tài liệu Báo cáo khoa học: Evolutionary divergence of valosin-containing protein ⁄cell division cycle protein 48 binding interactions among endoplasmic reticulum-associated degradation proteins ppt

... removal of the N-terminal, VBM-containing 16 amino acids of Ube4b blocks binding of the otherwise intact protein[8], this indicates similarities between the VCP-bindingsites of intact ERAD proteins ... for an additional leucine in Ube4b and Hrd1 and by the lack of VCP binding in OTUD7a and SMURF1 ⁄ 2.VBM dependence for VCP binding in intactproteins in vitro and in cells In order to conﬁrm a ... also in uence binding, and asimilar motif required for VCP binding in gp78 toler-ates substitution of these two arginines. We map the site of binding of Ube4b to the N-domain of VCP and show that...

Báo cáo khoa học: Evolutionary changes to transthyretin: structure and function of a transthyretin-like ancestral protein doc

... b-strands A, G and H [17].Structural nature of the TLP and transthyretin active sitesOne of the striking features of transthyretin is the cen-tral channel of the protein into which the THs bind.This ... involved in the oxidation of uric acid to allantoin. Certainly, the co-localization of these proteins in the peroxisomes of metazoan and plant species, and the co-regulation of TLP genes in some ... transthyretin dimers associate, vianonpolar interactions, between the loops joining standsG and H with the loops joining strands A and B, mak-ing the transthyretin tetramer a ‘dimer of dimers.’Recently,...

Tài liệu Báo cáo khoa học: Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by ﬂuorescence resonance energy transfer–ﬂuorescence lifetime imaging microscopy in living cells doc

... upon the distance between and relative ori-entation of two ﬂuorophores, being independent of the strength of protein–protein interactions. In the case of very weak interactions as described in ... between the N-terminal amino acids of subunits O and P,subunits O and Q and subunits O and R areshown (B–D). FLIM analysis of the interaction between cerulean–GAPDH and citrine–GAPDH in living ... that induction of FRET does not sim-ply depend on the length of linkers. In the linker con-taining seven amino acids, a Pro-Pro sequence that isnot contained in other linkers is incorporated....

Tài liệu Báo cáo khoa học: Post-translational modiﬁcation of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion pptx

... threonine kinasethat phosphorylates actin [12], binds the deubiquitinat-ing enzyme otubain 1 (OTUB1) [13,14], the smallG protein subunit Gaq [15] and interacts with mem-bers of the Rho family of ... Yersinia surface proteininvasin binds to b1 integrins on the apical surface of M cells, which facilitates translocation across the epithelium [4,5]. The pathogenicity and virulence of Yersiniae ... In addition to actin, other host cell proteins have beenshown to bind to the virulence factor YpkA, includingOTUB1 [13]. In order to investigate the role of OTUB1 in Yersinia invasion of HEK293T cells,...

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