Báo cáo khoa học: Puroindoline-a and a1-purothionin form ion channels in giant liposomes but exert different toxic actions on murine cells pptx

... compilation ª 2006 FEBS Puroindoline-a and a1-purothionin form ion channels in giant liposomes but exert different toxic actions on murine cells Paola Llanos 1 , Mauricio Henriquez 1 , Jasmina Minic 2 , ... native PIN-a and a1-PTH. PIN-a forms ionic channels in giant liposomes Seals of high-resistance and excised patches in an ‘inside out’ config...

Ngày tải lên: 07/03/2014, 12:20

... KOH, and 10 lm ionomycin. Electroporation of retinal cells for introducing iberiotoxin An E3 retina was positioned between a pair of platinum electrodes separated by 0.6 mm in NBS. A train of ... this idea, a 5 mm-K + solution containing nystatin (an ionophore for monovalent cations and Cl – ) was bath-applied to a DiOC 5 (3)-stained retina. It caused an increase in the DiOC 5 (3) fl...

Ngày tải lên: 30/03/2014, 10:20

... into the expression vector pFLAG1 at the unique NdeI and BglII sites. Expression of HRPC in E. coli W31110, isolation of inclusion bodies, refolding and purification of the wild-type protein and ... resulting from benzhydr- oxamic acid of concentration L, binding to a total protein concentration P, was determined, while allowing the remaining K d and maximum absorbance change at satu...

Ngày tải lên: 07/03/2014, 21:20

... glucose uptake in response to insulin [8,9]. Under basal noninsulin- stimulated conditions, GLUT4 was present mainly in the HD-caveolae (Fig. 2G), but increased in response to insulin in both the ... visualization of caveolin-1 in caveolae fractions. Caveolae fractions from density gradient ultracentrifugation were immunogold labelled against cave- olin-1 and examined by transmi...

Ngày tải lên: 16/03/2014, 13:20

... has in the resulting ICI-dimensional space then represents the collocational behavior of w in the training cor- pus. In the present implementation, IW[ 20,500 and ICI = 1000. For computational ... measuring cohesion. For want of a way of observing cohesion directly, this indirect relationship is commonly used for purposes of evaluation. 4 Implementation The implementation of t...

Ngày tải lên: 17/03/2014, 07:20

... Firestein GS (1991) Cytokines in chronic in ammatory arthritis. VI. Analysis of the synovial cells involved in granulocyte–macrophage colony-stimulating factor production and gene expression in rheumatoid arthritis ... Activation, differential localization, and regulation of the stress-activated protein kinases, extra- cellular signal-regulated kinase, c-JUN N-terminal kinase, a...

Ngày tải lên: 23/03/2014, 07:20

... (translation start site) in the second exon and with the bovine growth hormone transcription termination and polyadenylation signal (bGH). The first and second exon up to the translation start ... observed. Fig. 2. Main activity and interactions of GS upstream and intron modules in FTO-2B hepatoma and C2/7 muscle cells. Main activities and interactions (±95% confidence interv...

Ngày tải lên: 23/03/2014, 20:22

... binding. In addition, stimulation of endogenous EGF receptors promotes transient ERK1 ⁄ 2 and proliferation, and EGF receptor overexpression induces sustained ERK1 ⁄ 2 and promotion of differentiation. ... activators and targets, it is possi- ble that activation and its cell death-promoting func- tion may involve multiple partners and regulations [5]. ERK1 ⁄ 2 in neurodegenera...

Ngày tải lên: 29/03/2014, 08:20

... harvested and tested for pro- duction of cytokines. The data points at 0 h represent the concen- tration of cytokines in the serum 90 min after the glycolipid injection. Representative profiles obtained ... monitored by measuring cytokine secretion. The average of the duplicate cultures in one of the two experiments giving similar results is demonstrated. (C) Determin- ation of the cell...

Ngày tải lên: 30/03/2014, 08:20

... phosphoinositide binding [31]. The five-amino-acid insertion in the loop of Noxo1 might alter the configuration of the phospho- inositide-binding region, affecting the affinity for Expression and function ... (PX) domain in the N-terminus. PX domains occur in a variety of proteins involved in cell signaling, mem- brane trafficking, and polarity establishment, and func- tion as phosp...

Ngày tải lên: 19/02/2014, 06:20