Báo cáo khoa học: The catalysis of the SARS 3C-like protease is under extensive regulation by its extra domain doc

... extra domain FEBS Journal 273 (2006) 1035–1045 ª 2006 The Authors Journal compilation ª 2006 FEBS 1045 The catalysis of the SARS 3C-like protease is under extensive regulation by its extra domain Jiahai ... and deleted SARS 3C-like protease constructs This material is available as part of the online article from http://www.blackwell-synergy.com...

Ngày tải lên: 07/03/2014, 12:20

... of the ligands, OD2 of Asp165, NE2 of His79 and NE2 of His169, formed an equatorial plane. The other protein ligand, NE2 of His31, bound to the metal, in the company of a water oxygen, from the ... ApeSOD. This implies that the conformation around the active site of ApeSOD is independent of the presence of a metal cofactor. Figure 3D shows the superimposi...

Ngày tải lên: 14/02/2014, 22:20

... in the light of the results obtained in the present study. Rather, the involvement of manganese in the in vivo assembly of the metallocofactor of C. ammoniagenes R2F is envis- aged. This is based: ... RNR and that of other class 1 RNRs or, instead, is a result of the interaction of the R2F with the inactivated (oxidized) form of the R1E. We consider...

Ngày tải lên: 15/02/2014, 01:20

... concomitant loss of the electron density of the loop region between b3 and b4 near the Thr- binding site are observed. The mechanism of regulation by Thr is discussed on the basis of the crystal structures. ... regulatory domain away from the kinase domain. Accompanied by the rotation of the regula- tory domain, other loops from the catalytic domains...

Ngày tải lên: 18/02/2014, 08:20

... sup- press the activation (1000-fold induction) of the IFN-b gene promoter by TRIF (Fig. 7A). This result was also supported by the results of the dimerization analysis of IRF-3 (Fig. 7B). Furthermore, ... determine if its activa- tion is mediated by the TLR3 but not the TLR4 signaling pathway, we examined whether or not activa- tion of IRF-3 by M-pIC treatment...

Ngày tải lên: 18/02/2014, 16:20

... cleavage is masked by a conformational change. The simplest interpretation of this result is that the rate of substrate cleavage is not equivalent to the rate of chemistry, and that the rate of chemistry ... dissociation For the fraction of substrates that do undergo the reaction, the resultant products dissociate from the ribozyme relatively slowly on the...

Ngày tải lên: 18/02/2014, 18:20

... found at this position (see below). The southern boundary of the active site cleft of DESC1 is formed by the 145 autolysis loop. The backbone of this loop differs mark- edly from the other serine ... and these can be exploited by the design of specific inhibitors. To the west, the pocket is limited by Lys224 side chain. The flexibility of this side chain i...

Ngày tải lên: 19/02/2014, 00:20

... indicates the C-terminal part. The dimer’s formation is established by the incorporation of the b8-strand of one monomer into a b-sheet of the other monomer. The position of the zinc ion is indicated by ... at the surface of the hexamer. Figure 4 illus- trates that the complete active site cavity is formed at the level of the hexamer by two subu...

Ngày tải lên: 19/02/2014, 00:20

... related to the reduction of the PQ pool. Results As reported in the literature, the I step of the O–J–I–P fluorescence transient cannot be clearly distinguished by visual analysis of the FI traces ... when the temperature is raised. The effect of DCMU on the traces was to increase the amplitude of step J with the concurrent decline of step I, and to retard...

Ngày tải lên: 19/02/2014, 05:20

... They would be indistinguishable on the chromatograms reported by Grishutin et al., as the two octasaccharide isomers present in their product mixture also coeluted [6]. Further proof of hydrolysis at substituted ... 4, and may be the basis for the unique mode of action of this enzyme. More definitive statements await the solution of the Cel74A structure, which is curren...

Ngày tải lên: 19/02/2014, 05:20