Báo cáo khoa học: Iron regulatory protein-independent regulation of ferritin synthesis by nitrogen monoxide pot

... Iron regulatory protein-independent regulation of ferritin synthesis by nitrogen monoxide Marc Mikhael 1,2 , Sangwon F. Kim 2 , Matthias Schranzhofer 3 , Shan S. Lin 1,4 , ... and storage of iron. The regulation of ferritin synthesis is largely accom- plished via an elegant regulatory system that tightly controls intracellular iron levels. The structurally...

Ngày tải lên: 07/03/2014, 12:20

... determined by X-ray crystallography to a resolution of 2.5 A ˚ with a final R value of 23.3% and R free of 28.5% (Table 1). The enzyme is a hexamer composed of three dimers that are related by threefold ... and Phe133 (Fig. 3D). The central channel of the hexamer is made up of two layers of electrostatic forces on top of each other, with one layer rotated by 60°. The amin...

Ngày tải lên: 18/02/2014, 16:20

... equations. Normalization by dividing all substrate forms by [S] was used for estimation of k s and k )s ; normalization by dividing all concentrations by E tot wasusedtoinvestigatethe dependence of oscillations, ... general, disruption of the PAS architecture by mutation tends to increase the rate, k, of the hysteretic transition (k is the reciprocal of s), probably by i...

Ngày tải lên: 07/03/2014, 14:20

... solution of the nonionic surfactant Polysorbate 20. The purpose of the surfactant is to reduce sample loss caused by the adsorption of hydrophobic molecules to the surfaces of the flow system of the ... the absence of specific stabilizing structures. Because of their relatively broad spectrum of activity, it has been suggested that defence peptides may offer an alternative sou...

Ngày tải lên: 08/03/2014, 08:20

... Unit, School of Life Sciences, University of Dundee, UK 2 Division of Gene Regulation and Expression, School of Life Sciences, University of Dundee, UK Cisplatin and oxaliplatin are potent chemotherapeutic agents ... 3323 Results Identification of the protein encoded by YBL046w as a regulatory subunit of Pph3 The protein encoded by S. cerevisiae ORF YBL046w (SPTREMBL P381...

Ngày tải lên: 07/03/2014, 12:20

... characteristics of binding of PCSK9 to LDLR. Using PCSK9 iodinated by the tyramine cellobiose (TC) method ([ 125 I]TC-PCSK9), we measured the affinity and kinetics of binding of PCSK9 to LDLR ... The relative amount of cell-surface immunoreactive LDLR (A), as measured by the amount of specific binding of 125 I-labeled anti-(rabbit IgG) to cells, and the specific binding of [...

Ngày tải lên: 14/02/2014, 14:20

... PhyH-DII AB CD Fig. 2. Environmental factors that affect the catalytic activities of PhyH and PhyH-DII. (A) Effect of 0–5.0 mM Ca 2+ at 37 °C. (B) Effect of pH at 37 °C. (C) Effect of temperature at ... analysis of purified PhyH, PhyH-DI and PhyH-DII. Lane M, molecular weight markers; lane 1, culture supernatant of PhyH; lane 2, culture supernatant of PhyH-DII; lane 3, culture su...

Ngày tải lên: 14/02/2014, 15:20

... University of Bath, UK 2 Department of Biochemistry, University of Wisconsin–Madison, USA 3 Department of Chemistry, University of Wisconsin–Madison, USA Introduction Upon catalyzing the cleavage of ... regarding a new class of surrogates for the phosphoryl group. Results and Discussion Sulfonimides as inhibitors of RNase A We began by determining the ability of three back...

Ngày tải lên: 14/02/2014, 22:20

... K m ) of PRTFDC1 towards hypoxanthine and guanine was only 0.26% and 0.09%, respectively, of that of HPRT. This low activity could be explained by the fact that PRTFDC1 has a Gly in the position of the ... of the human HPRT homolog PRTFDC1. To shed light on the potential function of PRTFDC1, the protein was screened against a nucleotide metabolome library containing 81 potential...

Ngày tải lên: 15/02/2014, 01:20

... the development of better tools for glycan analysis by MS n techniques. We are currently building a spectral library of gly- can structures by measuring MS n spectra of a variety of glycans, as ... previously demonstrated the application of this method to the determination of the glycan structure of a form of AFP [10]. However, identification of the details of a glycan...

Ngày tải lên: 16/02/2014, 08:20