Báo cáo khoa học: Investigations into the ability of an oblique a-helical template to provide the basis for design of an antimicrobial anionic amphiphilic peptide pot
Báo cáo khoa học: Investigations into the ability of an oblique a-helical template to provide the basis for design of an antimicrobial anionic amphiphilic peptide pot
... with the anionic membranes of microbial cells, and they exert their antimicrobial action by the use of nonreceptor-based mechanisms of Keywords anionic; antimicrobial; a-helical; membrane; peptide Correspondence D. ... contribute to the susceptibility of E. coli model membranes to the action of AP1. An important determinant of the susceptibility of membr...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Saporin and ricin A chain follow different intracellular routes to enter the cytosol of intoxicated cells pptx
... Kreitman RJ & Pastan I (1995) Importance of the glu- tamate residue of KDEL in increasing the cytotoxicity of Pseudomonas exotoxin derivatives and for increased binding to the KDEL receptor. ... USA). Acknowledgements We thank Alexandre Mezghrani, Jez Simpson, Roberto Sitia and Tatiana Solda ` for the critical reading of this manuscript. We are grateful to Serena C...
Ngày tải lên: 23/03/2014, 15:21
Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt
... an important role in the regulation of cellular pro- cesses in plants and mammals. Moreover, they are an essential tool in the synthesis of phospholipids and phospholipid analogs. Knowledge of ... knowledge of their struc- tures cannot be generalized to plant PLDs. In this art- icle, the first information on the spatial structure of a plant a-type PLD has been obtained...
Ngày tải lên: 19/02/2014, 02:20
Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc
... 2004 significantly. The mutant E356Q had 38% and 50% of the activities for pullulan and panose, respectively. In contrast, the activity of E273Q for p ullulan was 45%, w hile that for panose remained ... isopanose from pullulan, and isomaltose and glucose from panose, and the action patterns of the wild- type and the W402F enzymes were almost identical (Fig. 2). The k...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... the existence of high and low affinity bindings of mangan- ese ions to arginase II and provide an explanation for the manganese stoichiometries determined here. Con- sidering the stoichiometry of 2 Mn 2+ Æsubunit )1 derived from ... His145Asn mutations on the affinity of metal binding to arginase II To further examine the effects of the His120fiAsn and His145fiAs...
Ngày tải lên: 20/02/2014, 02:21
Báo cáo khoa học: Insights into substrate and product traffic in the Drosophila melanogaster acetylcholinesterase active site gorge by enlarging a back channel ppt
... hypothesis, we used two inhibitors specific for the peripheral site that bind to Trp321 close to the entrance: propidium and aflatoxin B1. In the wild-type DmAChE, the affinity of propidium for the ... entrance of acetylcholine to the CAS in the wild- type enzyme, it appears that, in the two mutants (Trp83Ala ⁄ Glu), inhibitors that bind to the PAS did not preve...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Gene expression silencing with ‘specific’ small interfering RNA goes beyond specificity – a study of key parameters to take into account in the onset of small interfering RNA off-target effects potx
... post-transfection, and it decreased thereafter. Thus, according to our data, the undesired effects of siRNA seem to appear in a transient way. Another key parameter to take into account could be the length of the ... is taken into account in the normalization. Furthermore, in order to consider the purity and quality of the mRNA, a second normalization step was pe...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khóa học: Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using fluorescence spectroscopy pptx
... direct exploitation of the structural results of the former to explain the mechanism of CaM activation of the latter. Crystallization and resolution of the 3D structures of AC and AC–CaM, which ... 5¢-triphos- phate (Ant-dATP), than the free enzyme [22], and therefore a CaM-induced conformational change in the nucleotide- binding site was postulated. The recent r...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Insight into the phosphodiesterase mechanism from combined QM ⁄ MM free energy simulations pot
... oxyanion by His234. Although the MFEP in the two-dimensional PMF suggests that an intermediate is formed and there is a barrier for the proton transfer from the intermediate, the proton trans- fer ... contributing factor is the elonga- tion of the separation of the divalent metal ions as the reaction proceeds from the Michaelis complex to the transition state,...
Ngày tải lên: 14/03/2014, 23:20
Báo cáo khoa học: Investigations on the evolutionary conservation of PCSK9 reveal a functionally important protrusion pot
... in the binding of PCSK9 to the EGF-A domain of the LDLR, and mutations of these residues lead to loss of function, as found in our study and in the study of Kwon et al. [34]. The part of the ... and on PCSK9 cleavage by furin To study the effects of the 13 PCSK9 mutants on the PCSK9-mediated degradation of the LDLR, we used transiently transfected Hep...
Ngày tải lên: 23/03/2014, 07:20