Báo cáo khoa học: Identiﬁcation of tyrosine-phosphorylation sites in the nuclear membrane protein emerin pot

Báo cáo khoa học: Identiﬁcation of functional domains in the formyl peptide receptor-like 1 for agonist-induced cell chemotaxis doc

... the sixthtransmembrane domain and the third extracellular loop playing a promin-ent role. Interestingly, the N-terminus and a segment between the fourthtransmembrane domain and the third intracellular ... half of the ﬁrst TM domain through the second intracellular loop of FPRL1 with the corresponding part of FPR (A). Chimera H contains the N-terminus and a segment between the fourth TM and the ... control.Functional domains in FPRL1 Y. Le et al.774 FEBS Journal 272 (2005) 769–778 ª 2005 FEBS the involvement of multiple domains of FPR in recogni-tion of the agonist fMLF, including the ﬁrst, second,and...

Báo cáo khoa học: Identiﬁcation of domains involved in the allosteric regulation of cytosolic Arabidopsis thaliana NADP-malic enzymes ppt

... those of NADP-ME2 (Table 1). Theseresults indicate that, despite the differences detected, the binding sites for the substrates were integral in the chimeric proteins.Regulatory properties of the ... histidine tag to facilitate the puriﬁcation of the expressed fusion protein by a nickel-containing His-Bind column (Novagen, Gibbstown, New York, USA). The induction and isolation of the proteins ... than the other compounds (Fig. 6). In this way, amino acid res-idues of different segments of NADP-ME2 swapped in the construction of the chimeras are involved in the fumarate activation of the...

Báo cáo khoa học: Identiﬁcation of a domain in the a-subunit of the oxaloacetate decarboxylase Na+ pump that accomplishes complex formation with the c-subunit pot

... contains the carboxyl-transferase domain in the N-terminal part and the biotin-binding domain in the C-terminal part [5]. The b-subunit is an integral membrane protein with nine membrane- spanning ... 4A),indicating that amino acids 80–100 from the C termi-nus comprise part of the binding domain. This part of the protein is upstream of the putative linker segmentconnecting the C-terminal ... a-subunit contains the carb-oxyltransferase domain in its N-terminal portion and the biotin-bindingdomain in its C-terminal portion. The c-subunit plays a profound role in the assembly of the complex....

Báo cáo khoa học: Identiﬁcation of tyrosine-phosphorylation sites in the nuclear membrane protein emerin pot

... attachedto the lamina by integral membrane proteins of the INM. The INM proteins form complexes, transientlyor stably, with lamins, chromatin proteins and a vari-ety of regulatory proteins, including ... improving the search for phosphopeptidescontaining phosphotyrosine, we identiﬁed one of the well-deﬁned integral INM proteins. Emerin is a type-IIintegral membrane protein of 34 kDa, which is inser-ted ... several proteins undergo tyrosine phosphorylation at the nuclear envelope, we achieved, for the ﬁrst time, the identiﬁcation of tyrosine-phos-phorylation sites of a nuclear- membrane protein, emerin, ...

Báo cáo khoa học: Identiﬁcation of crucial residues for the antibacterial activity of the proline-rich peptide, pyrrhocoricin pot

... while the N-terminus served as the pharma-cophore and hence the interactive domain with the bacterialtarget protein, the C-terminal half aided the delivery of the pharmacophore into the interior ... pyrrhoco-ricin, indicating that the function of the C-terminal modulewas not compromised with the amino acid changes andinsertions in this domain. The removal of the Lys residuethat connected the ... drawn, the decrease of the intensity of the 200 nm band representing the unorderedstructure suggests the generation of a more orderedconformation. Identiﬁcation of the pyrrhocoricin binding surfaceonE....

Báo cáo khoa học: Identiﬁcation of amino acids in antiplasmin involved in its noncovalent ‘lysine-binding-site’-dependent interaction with plasmin pptx

... complement-ary site in antiplasmin is also of importance in regulating the ﬁbrinolytic system. The same sites in the plasmin moleculesare used in the interaction between plasmin and ﬁbrin [7].Fibrin-bound ... plasmin molecules [7,8]. In addition, it isknown that the lysine-binding sites in plasminogen areimportant for binding to other proteins, such as receptorproteins at the cellular surface, in ... picture of the structure of the C-terminalportion of antiplasmin. However, there are a large number of proline residues in this part (10 of the C-terminal 55residues), increasing the possibility of...

Báo cáo khoa học: Identiﬁcation of a copper-repressible C-type heme protein of Methylococcus capsulatus (Bath) A member of a novel group of the bacterial di-heme cytochrome c peroxidase family of proteins docx

... europaea CCPs. Strikingly, residues of both the heme-binding sites (CxxCH), in addition to the aminoacids coordinating the calcium ion present in the interface domain of the solved CCPs are ... 7C).Detection of C-type hemeBecause of the sequence similarity of SACCP to mem-bers of the BCCP family of proteins and the predic-tion of heme-binding motifs in the primary sequence,it was of interest ... periplasmic protein [18], hence indicatingthat the cells remained undisrupted during the extrac-tion procedure and thereby avoiding leakage of pro-teins from the periplasm. Protein immunoblot usingantibodies...

Tài liệu Báo cáo khoa học: Identiﬁcation of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt

... on the guanidini-um group of the Arg in the RGG domain in the C-terminal of EWS.Results and DiscussionSeveral DNA-binding proteins that bind to G-quadru-plex DNA have been investigated in ... RGG domain of nucleolin,a DNA-binding protein contributing to the transcrip-tion of ribosomal RNA, bind to the G-quadruplexforming ribosomal DNA [26]. Moreover, nucleolinbinds to the c-myc ... nucleolin [21–27]. The RGG domain of FMRP, which is an RNA-binding protein involved in nerve cell differentiation,interacts with the G-quartet forming RNA [22–25]. In addition, the RBD and the...

Tài liệu Báo cáo khoa học: Identiﬁcation of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc

... contained membrane- spanning regionsanchoring the proteins to the inner membrane but not in the cytoplasm of the bacterium (data not shown),which is consistent with those of deﬁned E. coli late Identiﬁcation ... bacteria. The difference betweenthese two mutants in bacterial clearance might reﬂectdifferences in the integrity of their outer membrane, binding activity and sensitivity of the murine comple-ment-mediated ... containing the various agents were plated on the lawn in triplicate at37 °C for 18 h. Sensitivity was assessed by measuring the diameter of the zone of growth inhibition in two axes, andthe...

Tài liệu Báo cáo khoa học: Identiﬁcation of differentially expressed genes of the Paciﬁc oyster Crassostrea gigas exposed to prolonged thermal stress docx

... tothermal stress are known to involve major adjustments in the expression of ribosomal genes and genes codingfor proteins involved in RNA metabolism and protein synthesis. In fact, protein ... assisting the folding of cytoskeletal proteins that are actively synthesizedand ⁄ or renatured under these conditions. The upregu-lation of all of these chaperones conﬁrms the severity of the thermal ... number of genes encoding structural components of the cytoskeleton and proteins involved in contractilefunctions (including actin, tubulin myosin and proﬁlin)were differentially expressed in C....

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