Báo cáo khoa học: Identification of tyrosine-phosphorylation sites in the nuclear membrane protein emerin pot

... the sixth transmembrane domain and the third extracellular loop playing a promin- ent role. Interestingly, the N-terminus and a segment between the fourth transmembrane domain and the third intracellular ... half of the first TM domain through the second intracellular loop of FPRL1 with the corresponding part of FPR (A). Chimera H contains the N-terminus and a segment b...

Ngày tải lên: 16/03/2014, 18:20

... those of NADP-ME2 (Table 1). These results indicate that, despite the differences detected, the binding sites for the substrates were integral in the chimeric proteins. Regulatory properties of the ... histidine tag to facilitate the purification of the expressed fusion protein by a nickel-containing His- Bind column (Novagen, Gibbstown, New York, USA). The inductio...

Ngày tải lên: 23/03/2014, 04:20

... contains the carboxyl- transferase domain in the N-terminal part and the biotin-binding domain in the C-terminal part [5]. The b-subunit is an integral membrane protein with nine membrane- spanning ... 4A), indicating that amino acids 80–100 from the C termi- nus comprise part of the binding domain. This part of the protein is upstream of the putative linker...

Ngày tải lên: 23/03/2014, 13:20

... attached to the lamina by integral membrane proteins of the INM. The INM proteins form complexes, transiently or stably, with lamins, chromatin proteins and a vari- ety of regulatory proteins, including ... improving the search for phosphopeptides containing phosphotyrosine, we identified one of the well-defined integral INM proteins. Emerin is a type-II integral membrane...

Ngày tải lên: 07/03/2014, 12:20

... while the N-terminus served as the pharma- cophore and hence the interactive domain with the bacterial target protein, the C-terminal half aided the delivery of the pharmacophore into the interior ... pyrrhoco- ricin, indicating that the function of the C-terminal module was not compromised with the amino acid changes and insertions in this domain. The removal...

Ngày tải lên: 08/03/2014, 10:20

... complement- ary site in antiplasmin is also of importance in regulating the fibrinolytic system. The same sites in the plasmin molecules are used in the interaction between plasmin and fibrin [7]. Fibrin-bound ... plasmin molecules [7,8]. In addition, it is known that the lysine-binding sites in plasminogen are important for binding to other proteins, such as recepto...

Ngày tải lên: 17/03/2014, 03:20

... europaea CCPs. Strikingly, residues of both the heme-binding sites (CxxCH), in addition to the amino acids coordinating the calcium ion present in the interface domain of the solved CCPs are ... 7C). Detection of C-type heme Because of the sequence similarity of SACCP to mem- bers of the BCCP family of proteins and the predic- tion of heme-binding motifs...

Ngày tải lên: 23/03/2014, 11:20

... on the guanidini- um group of the Arg in the RGG domain in the C-terminal of EWS. Results and Discussion Several DNA-binding proteins that bind to G-quadru- plex DNA have been investigated in ... RGG domain of nucleolin, a DNA-binding protein contributing to the transcrip- tion of ribosomal RNA, bind to the G-quadruplex forming ribosomal DNA [26]. Moreover, nucleol...

Ngày tải lên: 15/02/2014, 01:20

... contained membrane- spanning regions anchoring the proteins to the inner membrane but not in the cytoplasm of the bacterium (data not shown), which is consistent with those of defined E. coli late Identification ... bacteria. The difference between these two mutants in bacterial clearance might reflect differences in the integrity of their outer membrane, binding a...

Ngày tải lên: 18/02/2014, 14:20

... to thermal stress are known to involve major adjustments in the expression of ribosomal genes and genes coding for proteins involved in RNA metabolism and protein synthesis. In fact, protein ... assisting the folding of cytoskeletal proteins that are actively synthesized and ⁄ or renatured under these conditions. The upregu- lation of all of these chaperones confirms t...

Ngày tải lên: 18/02/2014, 16:20