Báo cáo khoa học: Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicans pot

... Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicans Elke Zameitat 1 , Zoran Gojkovic ´ 2, *, ... DHODH, the yields Fig. 1. Scheme of dihydroorotate dehydrogenase (DHODH) reaction with chemical formulae. Electron transfer from dihydroorotate to FMN and further on to quinone. Can...

Ngày tải lên: 07/03/2014, 12:20

... PCR’, using the first PCR product as template. To make use of the BamHI site in the vector and the XbaI site in the EhMGL1 gene, the product of the nes- ted PCR was replaced with the corresponding ... molecular ratio of 1 : 1000, and further tested for the Met-degrading activity on the basis of the detection of a-keto acid after the addi- tion of 2 mm Met (F...

Ngày tải lên: 07/03/2014, 05:20

... 8.5, we studied thermal stability of 17b-HSDcl from CD and UV melting curves, from which the temperature of denaturation (T d ) and the van’t Hoff enthalpy of denaturation (DH° vH )of1 7b-HSDcl were ... Inspection of Fig. 2B reveals two transitions, the first in the pH range pH 7–5, and the second in the pH range 5–3. The observed transitions in the aci- dic pH ran...

Ngày tải lên: 16/03/2014, 13:20

... 4 °C. The supernatant was transferred to an HPLC vial. The products of the reaction were detected at 260 nm using the HPLC system described above for the synthesis of the CoA- esters. The assay ... mUÆmg )1 protein). These results show, that the test measuring the hydratase reaction of AUH indeed is useful for the direct analysis of fibroblast cultures derived from...

Ngày tải lên: 19/02/2014, 07:20

... kunkelii, the genomes of the members of the mollicutes group (total number, 7 out of 10) are devoid of genes for the biosynthesis of IPP and DMAPP. The same is true for the genome of the archaeon N. ... of the enzyme for that cofactor and the apparent absence of a redox process as part of the catalytic cycle. The substrate binding site of the type I...

Ngày tải lên: 19/02/2014, 13:20

... products, the cleavage site protected by the presence of ubiquitin was Ser341, located in the ‘fingers’ region of the catalytic core domain involved in the recognition of the ubiquitin core. These ... bands of similar identity derived from other USP7 variants. These symbols were also used to represent graphically the cleav- age sites on the schematic view of USP7 sh...

Ngày tải lên: 07/03/2014, 05:20

... the presence of calcium were carried out with the addition of 5 mm CaCl 2 in the buffer. For investi- gation of the thermal denaturation of annexin B1, the CD signal at 222 nm was monitored from ... Whereas binding of the carbohydrate to the site on the concave surface of the protein most likely does not interfere with the essential annexin– membrane associat...

Ngày tải lên: 07/03/2014, 12:20

... been explored. To further characterize the enzymatic properties of plant TPP enzymes, we also purified recombinant OsTPP1 using the same methods. Then, the kinetic parameters of these recom- binant ... homogeneity. The size of the purified recombinant enzyme was estimated to be 45 kDa on SDS ⁄ PAGE, in accordance with the size deduced from the nucleotide sequence (42.6...

Ngày tải lên: 16/03/2014, 11:20

... properties of SPC4. The absorption spectrum of purified SPC4 was recorded in 50 m M sodium acetate pH 5. The inset shows the spectrum of the extract- ed heme. (B) MALDI-TOF-MS analysis of SPC4 heme. Characterization ... will obviously affect the shape of the active site, and thus either increase or decrease the enzyme’s affinity for substrate molecules [1]. This hypoth...

Ngày tải lên: 16/03/2014, 13:20

... Many of the tiss ues included in this a nalysis also showed a prevalence of one isoform over the other. For instance in the spleen, the short isoform is the predominant AK species , whereas in the ... than the cerebellum and various components of the forebrain. Furthermore, one or the o ther AK isoform predominates in most tissues, including the brain, where the sh...

Ngày tải lên: 16/03/2014, 18:20