Báo cáo khoa học: Stimulation of fibroblast proliferation by neokyotorphin requires Ca2+ influx and activation of PKA, CaMK II and MAPK/ERK pdf

... proliferation by neokyotorphin requires Ca 2+ influx and activation of PKA, CaMK II and MAPK/ERK Olga V. Sazonova, Elena Yu. Blishchenko, Anna G. Tolmazova, Dmitry P. Khachin, Konstantin V. Leontiev, Andrey ... activation should induce activation of all protein kinases shown to contribute to the neokyotorphin effect. Both PKA and CaMK II are known to be...

Ngày tải lên: 07/03/2014, 11:20

... no significant binding of actin was observed in any of the EREs, irrespective of the absence and presence of E2. Binding of ERa and ERb was increased in both ERE1 and ERE2 of the HOXC13 promoter ... II (RNAPII) and H3K4- trimethylation level in ERE1 and ERE2. Our results demonstrated that in both ERE1 and ERE2, the levels of RNAPII and H3K4-trimethylation were inc...

Ngày tải lên: 18/02/2014, 14:20

... be involved in stimulation of ENA1 expression and, conse- quently, in mediating Na + and Li + tolerance in yeast. Deletion of ISC1 distinctly decreased cellular Na + and Li + tolerance as growth of the ... extrusion of these cations by induction and activation of the specific, ATP-driven ion pump Ena1p is initiated [21,23–26]. Both pathways of yeast osmoadap- tation ha...

Ngày tải lên: 31/03/2014, 09:20

... PAP I. To better understand the mechanism of activation, we have investigated whether stimulation of PAP I activity is correlated with the affinity of Hfq for the 3¢ end of RNA. We used an RNA ... pooled and dialyzed Fig. 6. Hfq inhibits the addition of A residues by PNPase. Elonga- tion of 5¢ labeled polyadenylated 3¢rpsO mRNA by PNPase was per- formed in the absence and...

Ngày tải lên: 19/02/2014, 16:20

... activation of NF-jB. Attenuation of LXF-289 cell proliferation by ATP is mediated by the activation of the MEK ⁄ ERK1 ⁄ 2, PI3K and p38 MAPK pathways. Activation and translocation of the transcription ... II (CaMKII), which is also known to regulate cell cycle progression and proliferation. Inhibition of CaMKII activity by KN-62 (25 lm) completely reduced the...

Ngày tải lên: 07/03/2014, 12:20

... respectively. Plots and bars represent the means ± SD from three determinations. Fig. 11. Proposed models. (1) Activation of pNPPase by K + and pNPP. (2) Activation of pNPPase by Na + ,K + , pNPP and oligomycin. (3) ... simultaneous addition of NaCl and ATP induced convex-shaped activation curves of pNPPase. The combination of 10 mm NaCl and 0.1 mm ATP maximally...

Ngày tải lên: 07/03/2014, 16:20

... PASMC proliferation. Proliferation of PASMCs is a hallmark of pathogenesis of pulmonary hypertension [1,4]. How- ever, the mechanisms responsible for inhibition of PASMC proliferation by activation ... expression of HO-1 and p21 WAF1 . We also explored whether these responses modulate cell proliferation induced by 5-HT. Results Effect of PPARc agonist on HO-1 expres...

Ngày tải lên: 15/03/2014, 10:20

... Montan ˜ oLF&Lo ´ pez-Marure R (2005) Dehydroepiandrosterone inhibits the prolifera- tion of HUVEC by enhancing the expression of p53 and p21, restricting the phosphorylation of RB, and is androgen- and estrogen-receptor ... estrogen and androgen, because use of antagonists to androgen and estrogen receptors (flutamide and ICI 182,780, respectively), and letrozol, a...

Ngày tải lên: 16/03/2014, 00:20

... which suggested that stimulation periods of less than 2 min caused only partial activation and reversible relocation of cPLA 2 -a Fig. 5. Effects of EGTA and BAPTA-AM on the relocation of cPLA 2 -a. ... of CaCl 2 and EGTA to Buffer B, as determined by the METLIG program. Equivalent amounts of the fractions were analysed by SDS/PAGE and Western blotting. Temperature-...

Ngày tải lên: 30/03/2014, 13:20

... characterized by a conserved DFD amino acid motif in subdomain VII of the catalytic domain and by the presence of an amino acid insertion of variable size between subdomains VII and VIII [8,9]. Such ... and GST- lofMPK6. However, when lofMPK3 and lofMPK6 proteins were tested for phosphorylation with OXI1, no phosphorylation of lofMPK3 or lofMPK6 was observed (Fig. 5A). On...

Ngày tải lên: 14/02/2014, 19:20