Báo cáo khoa học: Purification, microsequencing and cloning of spinach ATP-dependent phosphofructokinase link sequence and function for the plant enzyme pdf

... spinach ATP-dependent phosphofructokinase link sequence and function for the plant enzyme Christian Winkler, Britta Delvos, William Martin and Katrin Henze Institute of Botany III, University of Du ¨ sseldorf, ... [22] reported the existence of chloroplast- and cytosol-specific isoenzymes in spinach. Since then, the isoforms of ATP-PFK from various plant...

Ngày tải lên: 07/03/2014, 11:20

... to the anchor sequence combined to the 3¢-end of the oligo- dT primer for first-strand synthesis of cDNA [12], and 5¢-RACE amplification was performed with a gene-specific primer based on the sequence ... in the sequence (Fig. 3). Genomic PCR experiments with a variety of different primers designed from the cDNA sequence resulted in the amplification of bands in...

Ngày tải lên: 17/03/2014, 10:20

... from cleavage of the signal peptide and the propeptide, which happens in the maturation process for every conotoxin [8], the removal of the two additional resi- dues Leu and Val at the C-terminus is rather ... with the presence of a short 3 10 -helix. A random coil rather than a compact loop in the region of residues 1–10 existed in l-Phe13-conomarphin. Fig. 3....

Ngày tải lên: 18/02/2014, 17:20

... Genes, enzymes and regulation of arginine biosynthesis in plants. Plant Physiol Biochem (in press). 19 Arabidopsis Genome Initiative (2000) Analysis of the genome sequence of the flowering plant ... CLGAT5a for the first PCR and CLGAT5b for the second PCR. For 3¢-RACE, single-stranded cDNA was synthesized using First-Strand cDNA Synthesis Kit (Amersham Bioscience)...

Ngày tải lên: 20/02/2014, 03:20

... middle and C-terminal region of the HdEG66, respectively, from the sequence similarity to the termite cellulase. Then, a forward primer F1 was synthesized on the basis of the N-terminal sequence of the ... sides of the cDNAsindicateprimersusedforthePCR.LengthsofthecDNAsare shown in the parentheses. (B) Structure of the genomic fragment, Hdcel-1 DNA. Open and...

Ngày tải lên: 20/02/2014, 23:20

... constit- uents of the cell surface. They are found at the outermost ends of the sugar chain of animal glycoconjugate. They act as an important component of the ligands recognized by the lectins. Recognition ... gently at room temperature for 3–4 h. The degree of coupling was checked by the reduction of BSM in the coupling medium. The protein concentration in t...

Ngày tải lên: 21/02/2014, 00:20

... physiological electron acceptor of the enzyme. The enzyme was not oxidized by the heterodisulfide of coenzyme M and coenzyme B, or by the homodisulfides of these two coenzymes. Also potassium trithionate and sodium ... out in the presence of one of the substrates of the enzyme. The physiological electron acceptor of the A. ful- gidus enzyme is still unk...

Ngày tải lên: 21/02/2014, 03:20

... kDa product. Furthermore, their 36.5 kDa bands became broader than that for the 50 min incubation. The sequence analyses of the 36.5 kDa bands revealed that two sequences of further digested products ... where most of cross-links are located, and swelled the egg envelope. Therefore, the manner of egg envelope diges- tion is analogous between ZHE1 and MHCE. The cros...

Ngày tải lên: 07/03/2014, 04:20

... three of them were N-terminally sequenced. The observed microhet- erogeneity in the N-terminal and cDNA sequences reveals the presence of several distinct cysteine proteinase isoforms in the latex ... content in their latex; and (2) the pres- ence of other cysteine proteinases or isoforms in the latex. To investigate these two hypotheses, the protein concentration per...

Ngày tải lên: 07/03/2014, 11:20

... cDNA sequence and sequence alignment of Cyn d 24. (A) Nucleotide and deduced amino acid sequences of Cyn d 24. The numbers on the right of the figure indicate the positions of the nucleotide sequence. ... this partial sequence, two specific primers were designed and used together with the anchor pri- mer AP2 to obtain the 5¢ and 3¢ portions of the Cyn d 2...

Ngày tải lên: 07/03/2014, 12:20