Báo cáo khoa học: DNA polymerase e associates with the elongating form of RNA polymerase II and nascent transcripts pot

... lysates were derived from the cerebellum. Colon lysates were derived from the descending colon. Heart lysates were derived from the left ventricle. Muscle lysates were derived from skeletal muscle ... the plasma mem- brane in the presence of KCNQ1. All lysates were probed with anti- HA to demonstrate the presence of KCNE4. (C) KCNQ1 traffics to the plasma membrane in the...

Ngày tải lên: 18/02/2014, 17:20

... counts presented in the 2 × 2 contingency table of Figure 1. For gen- eral evidence, we averaged across all experiments using relative frequencies. Note that the relative frequencies of positives (TP ... are often considered as the smallest unit of a language when examining the grammatical structure or the meaning of sentences, referred to as syntax and semantics, however,...

Ngày tải lên: 20/02/2014, 04:20

... the expectation that the second time the task is per- formed the subject does better, half the subjects begin with natural language, while half the subjects begin 14C with the alternative ... ' ~e are computer workers and therefore well placed to know the present and future dangers of computer systems. They are used to classify, control and to repress.&qu...

Ngày tải lên: 21/02/2014, 20:20

... protected or if they degrade within the biological environment. We have studied the effects of QDs on living cells and have reported their internalization, the intracellular production of reactive ... of CdTe QDs. (E) The mean number of lipid droplets per microscopic field evaluated as described in Materials and methods. The data represent the mean and SEM from two in...

Ngày tải lên: 07/03/2014, 00:20

... can deduce that 10 °C corresponds closely to the tempera- ture transition between the bent form and the linear form, since their populations are roughly equivalent. The agreement between our results ... stiffness experience part of the SRE internal movements. If the conversion between a bent and a linear form occurs at a shorter or longer timescale than the fluorescenc...

Ngày tải lên: 07/03/2014, 09:20

... depletion of H3K4Me2 in the regions of DNA methylation. Conversely, the level of H3K4Me2 remains high in the unmethylated DNA regions regardless of the presence of RNA Pol II. It can be proposed that Me CpG- ... promoters of silenced gene during cancer development [16]. Catalytic mechanisms of DNMTs involve the formation of a covalent bond between a cysteine r...

Ngày tải lên: 18/02/2014, 14:20

... MluI, and NotIas landmark enzymes. These four enzymes cleave specific GC-rich sequences and therefore gave satisfactory resolution of a sufficient number of spots because the genomes of all three bacterial ... The cleavage by the landmark enzymes (AscI, MluI, and NotI) is not affected by CcrM methylation because of the lack of GANTC sequences on their recognition sequenc...

Ngày tải lên: 19/02/2014, 02:20

... depending on the nucleotide sequence of the given target site. The relative sensitivities of protein DNA binding to cisplatin DNA treatment correlated with the occurrence of sequence motifs forming ... 2A) whose mobilities reflected different molecular weights of the p73 isoforms. To verify the specificity of the band shifts for DNA complexes with the proteins...

Ngày tải lên: 19/02/2014, 05:20

... barriers in the reaction pathway. The FRET chan- ges observed were indeed related to stable changes associated with DNA in the strand exchange reaction as they were stable to the steps of protein ... line 3) reflects genuine FRET change associated with strand renaturation. Homology dependence of renaturation activity In the following experiment, we examined the homol- ogy...

Ngày tải lên: 19/02/2014, 07:20

... delete site I), C and D (to delete site II) , E and F (to delete site III), G and H (to delete the perfect inverted repeat) and I and F (to delete simultaneously sites II and III) were ligated ... Further single replacements within each binding site (I, II or III) may reveal the essential role of single nucleotides in the specific inter- action with SenR. The pos...

Ngày tải lên: 07/03/2014, 10:20