Báo cáo khoa học: Human skin cell stress response to GSM-900 mobile phone signals In vitro study on isolated primary cells and reconstructed epidermis docx

... compilation ª 2006 FEBS 5507 Human skin cell stress response to GSM-900 mobile phone signals In vitro study on isolated primary cells and reconstructed epidermis Sandrine Sanchez 1 , Alexandra Milochau 2 , ... T the temperature in Kelvin and t the time in seconds. In line with the International Commission on Non-Ionizing Radiation Protection g...

Ngày tải lên: 07/03/2014, 11:20

... proteins released by it in the surrounding medium [3]. Major virulence factors that contribute to the in ammatory response and to epithelial cell damage have been iden- tified, among them cytotoxin-associated ... is among the proteins that play a relevant role in bacterial colonization and persistence in the stomach. Indeed, it was demonstrated to be overexpressed under ac...

Ngày tải lên: 16/02/2014, 14:20

... transcription in response to mtDNA depletion and repletion in HeLa cells. Nucleic Acids Res 30, 1929–1934. 13 Fisher RP & Clayton DA (1988) Purification and characterization of human mitochondrial ... of total protein) from the parental cell line (T-Rex), un- induced (2462 unind) and induced (2462 ind) 2462 cell line as well as the mitochondrial fraction (Mito) from th...

Ngày tải lên: 07/03/2014, 05:20

... techniques Recombinant proteins. Recombinant proteins were separ- ated on 15% SDS/PAGE, under reducing and nonreducing conditions (approximately 1 lg) for the silver staining and under nonreducing conditions ... positive in uence. To further evaluate the role of b-chain CCP2 in the interaction with protein S we decided to introduce amino acid changes into putative binding sites...

Ngày tải lên: 17/03/2014, 09:20

... 5 ¢ -and3 ¢-ends fully complementary to the hairpin flanking sequences, was used to monitor the primer extension reaction conditions; and primer 2, with its 3¢-terminus located in the hairpin stem, ... as selected on Luria–Bertani agarose plates containing isopro- pyl thio-b- D -galactoside and X-gal was isolated, and the orientation of the insert in M13mp19 was determined by...

Ngày tải lên: 23/03/2014, 13:20

... SKAR, including the amino acids of the RNA recognition motif (RRM) also known as the RNA binding domain (Fig. 2) [26]. Interaction of the human proteins ER and PDIP46/SKAR in vitro In order to confirm ... other functions. Contrary to the study on the Xen- opus ER, we have found that the human ER predom- inantly localizes to the nucleus of mammalian cells, including HeLa...

Ngày tải lên: 19/02/2014, 05:20

... immune responses. Several viruses have been demonstrated to interfere with antigen processing and presentation by infected cells, through inhibition of peptide recruitment to and processing by ... antigen presentation about five-fold and impair T -cell- mediated killing in vitro, and may there- fore be sufficient to explain the failure of E7-expressing skin to be rejected...

Ngày tải lên: 29/03/2014, 00:20

... DNA and a histone octamer, is complemented with linker histone (H1) and an additional 20 DNA bp to form the chromato- some. Linker DNA completes and links consecutive nucleosomes which fold into ... inter-nucleosomal interactions. The last region was interpreted to reflect extension of the beads -on- a-string fiber. The incorporation of the linker histone had only a minor effect on...

Ngày tải lên: 14/02/2014, 18:20

... to bind both essential and nonessential metal ions in vivo and in vitro strongly suggests a role in metal ion storage, metabolism and trafficking of Cu and Zn, as well as sequestration of Cd and ... that upon loss of the first metal ion, the three remaining metal ions in the a domain rear- range, either independently or in conjunction with the b domain, to position ano...

Ngày tải lên: 19/02/2014, 00:20

... proliferating and nonproliferating cells. First, we analysed the expression and subcellular distribution of human Cdc45 protein during the cell cycle in proliferating HeLa S3 cells. To this end, cells were ... [3]. In contrast to quiescent cells, senescent cells fail to initiate DNA replication in response to mitogens [4]. Previous reports have shown that licens...

Ngày tải lên: 19/02/2014, 00:20