Báo cáo khoa học: Human recombinant prolidase from eukaryotic and prokaryotic sources Expression, purification, characterization and long-term stability studies pptx

... multidomain structure of ceruloplasmin from calorimetric and limited proteolysis studies. J Biol Chem 265, 21016–21022. 35 Durao P, Chen Z, Fernandes AT, Hildebrandt P, Murgida DH, Todorovic S, Pereira ... antifer- romagnetically coupled through a bridging ligand and therefore EPR silent, show a characteristic absorption band at 330 nm. The T2 site lacks strong absorption bands, and...

Ngày tải lên: 18/02/2014, 04:20

... transfer format between sources other than SABIO-RK. Derived from the SABIO-RK database schema [23], it comprises kinetic laws, parameters and relevant metadata in a structured and standardized for- mat, ... means of identifying species, reactions and parameters across each of these data resources. Data submitted from the kineticswizard populates both databases, and from this,...

Ngày tải lên: 18/02/2014, 04:20

... values from duplicate determi- nations from a single experiment (data for 0.1, 5 and 10 n M) and the mean ± SD from three independent experiments each with duplicate determinations (data for 0.5 and ... purchased from ATCC (Rockville, MD, USA) and soluble TM was produced as described previously [42]. Human prothrombin was purchased from Enzyme Research Laboratories, Kordia...

Ngày tải lên: 18/02/2014, 06:20

... essentially random phosphorylation and dephosphorylation. Combinations of random and sequential mechanisms are possible. For example, it is conceivable that phos- phorylation of a protein is random ... of conformations available to the ligand, and leading to a sigmoidal change in the fraction of bound ligand as a function of its phosphorylation state. Effective binding of the ligand wou...

Ngày tải lên: 18/02/2014, 08:20

... SDS sample buffer, and the mixture was subjected to immunoblotting. Acknowledgements B. Vojtesek and R. Hrstka are funded by grants 301 ⁄ 05 ⁄ 0416 and 301 ⁄ 08 ⁄ 1468 from GACR and grant LC06035. ... well as primary human cancers, rather than just cell lines and cDNA from the NEDO human sequencing project. We evaluated the expression of the mRNAs encompassing full-length DA...

Ngày tải lên: 18/02/2014, 17:20

... expression of human Cdc45 in proliferating and nonproliferating cells. Our findings show that Cdc45 protein is absent from long-term quiescent, terminally differentiated and senescent human cells, although ... whether the human replication factor Cdc45 is also downregulated when human cells exited from the cell cycle and entered into the G 0 phase. Therefore, T98G glioblast...

Ngày tải lên: 19/02/2014, 00:20

... spectrometry A protein band was excised from a gel and analyzed by tandem mass spectrometry at Laboratory of Mass Spectr- ometry, Institute of Biochemistry and Biophysics PAS, Warsaw, Poland. Briefly, after ... the ER and POLDIP3 genes. Array of 76 polyA + RNA samples isolated from various human adult and fetal tissues and cancer cell lines was hybridized with the radiolabeled...

Ngày tải lên: 19/02/2014, 05:20

... Meeting, PA), Sephadex G-25 and G-50 from Amersham Biosciences (GE Healthcare, Piscataway, NJ), Cleland’s reagent (dithio- threitol) from Calbiochem (San Diego, CA), and Zinquin ester from Molecular Probes ... 83–88. Aldehydes and zinc metabolism Q. Hao and W. Maret 4310 FEBS Journal 273 (2006) 4300–4310 ª 2006 The Authors Journal compilation ª 2006 FEBS Aldehydes release zinc...

Ngày tải lên: 19/02/2014, 06:20

... mechanistic and crystallographic studies have been carried out with the E. coli and human protein [1]. Epimerase has also been purified and analyzed from the yeast Kluyveromyces fragilis [4–6] and Saccharomyces ... UDPgalactose 4-epimerase from S. cerevisiae is described in the Materials and methods section and also summarized in Table 1. Either the wild- type or the recom...

Ngày tải lên: 19/02/2014, 12:20

... On the other hand, the Type 1 gene is lacking in a large number of prokaryotic genomes, and distribution of the gene in prokaryotic genomes is not apparently correlated with the prokaryotic evolutionary ... mL )1 ampicillin and 30 lgÆmL )1 chloramphenicol, and incubated at 30 °C and 42 °C. Plasmid constructions, overproductions and purifications Plasmids for overexpression of H...

Ngày tải lên: 19/02/2014, 18:20