Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx

... Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition Karuppiah Chockalingam 1, *, ... rela- tionships of human manganese superoxide dismutase. Abbreviations FeSOD, iron superoxide dismutase; hMnSOD, human manganese superoxide dismutase; MnSOD, manganese superoxide...

Ngày tải lên: 07/03/2014, 11:20

... specifici- ty of ESP (Table 2) and the increased ESP activity observed in conjunction with reduced myrosinase activity at lower temperatures (Table 1). Because the putative substrates of ESP and NSP are highly ... min final hold). MS and FID were carried out as described previously [15]. Products were identified by comparison of mass spectra and retention times with those...

Ngày tải lên: 23/03/2014, 11:20

... D60, E63) and ⁄ or at both ends of VpUcyt (S38, V78, D79, D80) in the disallowed region of the Ramachandran plot. Superposition of the 20 lowest energy conformers yielded a narrow bundle of backbone ... (HSQC) spectra with enhanced spectral dispersion and the expected number of cross-peaks of VpUcyt were obtained with perdeuterated DPC (DPC-d38) at 30 °C. A series of HSQ...

Ngày tải lên: 18/02/2014, 06:20

... salt bridge with Arg47 (distance of 3.0 A ˚ ), an electrostatic interaction with the Zn 2+ ion (closest distance of 4.1 A ˚ ) and an extensive network of hydrogen bonds involving a set of well-ordered ... orientation of Arg47, and is ‘edge-on’ oriented with respect to the DHAP aliphatic chain with a shortest distance of 3.7 A ˚ from C1 (Fig. 3B). Such a conformation is...

Ngày tải lên: 18/02/2014, 06:20

... time span of the assay was 2 weeks using a rela- tively high concentration of LlChi18A and LlCBP33A (1.0 and 3.0 lm, respectively). The production of (GlcNAc) 2 (the major end -product of enzymatic hydrolysis ... after 2 min of incubation with 1 n M of enzyme. The double peaks represent the a- and b-anomers of the oligomers. Using standard curves, the total concentrat...

Ngày tải lên: 18/02/2014, 08:20

... of STAT3 and colocalizes with STAT3 to the cytoplasm of SW480 cells The transcriptional activity of STAT3 after treatment of the cells with hpST3dODN was analysed in SW480 cells transfected with ... control ODN (con) and the dead cells were counted after 16, 24 and 48 h of culture using trypan blue staining. (C) Cells were treated with 0.5, 1 and 2 lg of hpST3dOD...

Ngày tải lên: 18/02/2014, 08:20

... those for each of the MCAD mutants, K364R and R256T, and for the wild-type enzyme, in E. coli cells grown both at 31 °C and 37 °C, and the effect on activity (ferri- cenium assay), and tetramer ... (Fig. 3B), and, as expected, were very A B Fig. 2. Comparison of (A) western blot (showing the amount of tetramer formed) and (B) activity stain (showing the activity leve...

Ngày tải lên: 20/02/2014, 02:21

... additional hydrogen bonding with b-strand A5 and the extension of b-strand B3 interacts with a b-strand B4. BAX also has a longer C-terminal b-strand A4 and a short additional b-strand after that. When ... al.[8],theshapeofthemoleculeresemblesa right hand: two b-sheets and the a-helix form fingers and a palm, a long loop between the B7 and B8 strands forms a thumb, and a loop...

Ngày tải lên: 20/02/2014, 23:20

... one of the most abundant enzymes in the biosphere [1,2]. It consists of the Fe-protein (dinitrogenase reductase, also referred to as NifH), an a 2 dimer of the nifH gene product and of the MoFe-protein ... digestions of the NifH protein with trypsin and endoproteinase Asp-N. A peak of 3764.5 m ⁄ z corresponding to the ADP-ribosylated peptide 87–117 could not be observed in...

Ngày tải lên: 07/03/2014, 02:20

... respect to the role of HSP70 in the inhi- bition of the release of Smac and apoptosis. The role of the ATP-binding domain of HSP70 in the prevention of the release of Smac and apoptosis after ... domain of HSP70 is essential for the inhibition of H 2 O 2 -induced activation of caspases-9 and -3 and apoptosis. (A) The effects of HSP70 and its deletion mutant p...

Ngày tải lên: 16/03/2014, 01:20