Báo cáo khoa học: Solution structure of the catalytic domain of RICH protein from goldfish pot

... to the magnesium binding site M3 [4]. Fig. 4. The influence of Mg 2+ on the kinetic properties of APase from E. coli. The influence of Mg 2+ on the kinetic properties of APase from E. coli inthepresenceofaZn 2+ to ... catalysis of both subunits. Model representation of the catalytic cycle for APase from E. coli A model describing the catalytic mechanism of...

Ngày tải lên: 21/02/2014, 00:20

... for various adaptor proteins [6–10]. ClpA consists of three domains: an N -domain and two ATP-binding domains referred to as the D1 and D2 domains. Interestingly, deletion of the N -domain from ClpA not ... that the N -domain controls bind- ing of ClpA to ClpP [14]. Interestingly, there is also considerable debate regarding the role of the ClpB N -domain (which shares...

Ngày tải lên: 07/03/2014, 05:20

... mecha- nism of transphosphorylation, which implies the A B Fig. 3. Stereo diagram of the active center of HtA. (A) Superposition of the active-site residues of the 20 conformers of the solu- tion structure ... electrostatically with the other charged groups. In the NMR structures, this side chain of D40 is a short distance (£ 3A ˚ ) from the side chains of...

Ngày tải lên: 18/02/2014, 08:20

... cluster in these two regions. Know- ledge of the structure of a larger portion of MeCP2 including the transcriptional repression domain would resolve these speculations and clarify the role of the helical ... [15]. The solution structure of the MBD of rat (r)MeCP2 has recently been determined [17]. The MBD adopts a wedge- shaped structure, composed of an a...

Ngày tải lên: 21/02/2014, 00:20

... al. Solution structure of the RICH catalytic domain FEBS Journal 274 (2007) 1600–1609 ª 2007 The Authors Journal compilation ª 2007 FEBS 1609 Solution structure of the catalytic domain of RICH protein from ... close to its Fig. 2. Structure of goldfish RICH catalytic domain. (A) Stereo view of the backbone superposition of 10 lowest energy s...

Ngày tải lên: 07/03/2014, 10:20

... towards the interior of the protein. The space that in the wild-type molecule is occupied by the large hydrophobic side chain of Fig. 3. Structure ensemble of HPr(I14A). The average structure of the 10 ... for the calculations. Therefore, we recalculated the structure of the w ild-type protein on the b asis of the restraints used previously [15] wi...

Ngày tải lên: 07/03/2014, 16:20

... further our knowledge with respect to the mechanisms of action of these proteins, it is crucial that we define the precise boundaries of the STI1-homologous domain and compare the struc- tures of ... N-terminal part of XPCB–hHR23B ABC Fig. 2. NMR structure of the XPCB domain of hHR23B. (A) Stereoview of the 12 superimposed structures of XPCB–hHR23B. All Pro...

Ngày tải lên: 07/03/2014, 17:20

... fact, in the structures of Crt, the side-chain of Phe 25 is close enough to the amide proton of Lys38 to induce a ring current effect. Description of the toxin structure and potential surface The ... reported in Fig. 3A, and the analysis of the / and w angle distribution allowed the identification of the type and boundaries of the secondary structure eleme...

Ngày tải lên: 20/02/2014, 11:20

... the condensation and fragmentation of their nuclei. The percentage of apoptotic cells was calculated as the number of apoptotic cells divided by the total number of cells counted. A minimum of ... still largely unknown. The aim of the current study was to investigate the potential involvement of nucleolin ⁄ C23 in the antiapoptotic mechanism of Hsp70. We found...

Ngày tải lên: 16/02/2014, 09:20

... volumes of 5 M urea solution and then run on SDS ⁄ PAGE. Lanes a and d, in the absence of both TnC and Ca 2+ ; lanes b and e, in the presence of TnC and the absence of Ca 2+ ; lanes c and f, in the ... cation. Therefore, this interaction potentially participates in both the Ca 2+ -dependent activation of the contraction and the maintenance of structural integrity...

Ngày tải lên: 20/02/2014, 01:20