Báo cáo khoa học: An acridone-producing novel multifunctional type III polyketide synthase from Huperzia serrata pot
Báo cáo khoa học: An acridone-producing novel multifunctional type III polyketide synthase from Huperzia serrata pot
... 2007 FEBS
Multifunctional type III PKS from Huperzia serrata K. Wanibuchi et al.
An acridone-producing novel multifunctional type III
polyketide synthase from Huperzia serrata
Kiyofumi Wanibuchi
1
, ... a novel plant type III polyketide synthase was cloned
and sequenced from the Chinese club moss Huperzia serrata (Huperzia-
ceae). The deduced...
Tài liệu Báo cáo khoa học: Functional expression and mutational analysis of flavonol synthase from Citrus unshiu pptx
... (lane 3),
Gly261fiAla (lane 5), Gly68fiAla (lane 7), Gly68fiPro (lane 9) and
Gly261fiPro (lane 11), respectively, as well as the solubilized pellet
fractions of these wild -type (lane 2) and mutant ... Gly68fiPro and Gly261fiPro
mutant extracts (lanes 9 and 11).
Table 2. Specific activities of the wild -type and the Gly68fiAla, Gly68fiPro, Gly261fiAla, Gly261fiPro and Pro207fiGly mutant flavonol
synthas...
Báo cáo khoa học: Characterization of a thiamin diphosphate-dependent phenylpyruvate decarboxylase from Saccharomyces cerevisiae potx
... decarboxylase from
Saccharomyces cerevisiae
Malea M. Kneen
1
, Razvan Stan
1
, Alejandra Yep
2
, Ryan P. Tyler
2
, Choedchai Saehuan
2,
* and
Michael J. McLeish
1
1 Department of Chemistry and Chemical ... 2-ketopentanoic acid.
Each variant has a K
m
value less than 1 mm, which is
lower than that of the wild -type enzyme, as well as
k
cat
⁄ K
m
values that are greater than that of the wild...
Tài liệu Báo cáo khoa học: An alternative isomerohydrolase in the retinal Muller cells of a cone-dominant species doc
... reviewing the manuscript. This study was
supported by NIH grants EY018659, EY012231 and
EY019309; a grant (P20RR024215) from the National
Center for Research Resources; and a grant from
OCAST.
References
1 ... specificity
of the antibody was confirmed using recombinant
RPE65c, human RPE65, zebrafish RPE65a and
13cIMH. As shown by western blot analysis, the anti-
body specifically recognized R...
Tài liệu Báo cáo khoa học: An anthrax lethal factor mutant that is defective at causing pyroptosis retains proapoptotic activity pdf
... 9500 g for 30 min, and
the supernatant was decanted. The pellet was resuspended in
10 mL of supernatant, and then centrifuged at 20 000 g for
30 min. The supernatant was decanted, and 10 mL of 20 ... activities of wild-
type LF and LF-K518E ⁄ E682G in a cytotoxicity assay
using this melanoma cell line. PA and either wild -type
or mutant LF were incubated with MALME-3M cells
for 72 h, and v...
Tài liệu Báo cáo khoa học: An autoinhibitory effect of the homothorax domain of Meis2 ppt
... using an Absolutely RNA kit
(Agilent, Santa Clara, CA, USA). For quantitative RT-PCR,
cDNA was generated using Superscript III (Invitrogen), and
analyzed by PCR using a DNA engine cycler and Promega
Taq. ... shown are human Meis1, Meis2, Meis3, Prep1,
and Prep2, Xenopus laevis Meis1, Meis3, and Prep (XlMs1, XlMs3, and XlPrep), Drosophila melanogaster HTH (DmHth), and a Meis-like pro-
tein...
Tài liệu Báo cáo khoa học: An allosteric DNAzyme with dual RNA-cleaving and DNA-cleaving activities doc
... conserved. A12 can be
changed to T12, and A15 can be changed to G15.
When 8–17 DNAzyme binds its substrate, a gÆT
wobble pair can be formed, and is considered to be
significant and crucial for the ... An allosteric DNAzyme with dual RNA-cleaving and
DNA-cleaving activities
Dazhi Jiang*, Jiacui Xu*, Yongjie Sheng, Yanhong Sun and Jin Zhang
Key Laboratory for Molecular Enzymology and Engineeri...
Tài liệu Báo cáo khoa học: An ecdysteroid-inducible insulin-like growth factor-like peptide regulates adult development of the silkmoth Bombyx mori docx
... by Grants-in-aid for Scientific
Research (20570056) from the Ministry of Education,
Culture, Sports, Science and Technology of Japan.
N. Yamanaka was a recipient of a research fellowship
from ... organizations and physiological func-
tions: insulin is a heterodimeric peptide consisting of
an A-chain and a B-chain, whereas IGFs are single-
chain peptides with domains B, C, A and D, and .....
Tài liệu Báo cáo khoa học: TransLISA, a novel quantitative, nonradioactive assay for transcription factor DNA-binding analyses pdf
... basic
biological and drug discovery ramifications, there is
an increasing demand for quantitative, fast and high-
throughput assays of transcription factor function
with improved sensitivity and increased analytical
range.
Keywords
DNA-binding ... for the study of any transcription factor.
Thus, TransLISA can replace EMSAs and may be used in various applica-
tions and research fields where qu...
Tài liệu Báo cáo khoa học: An intermediate step in the evolution of ATPases – a hybrid F0–V0 rotor in a bacterial Na+ F1F0 ATP synthase pdf
... 1).
Fig. 1. Isolation and subunit composition of
the c rings from the Na
+
F
1
F
0
ATP synthase
from A. woodii. Samples of isolated enzyme
(lane 1) and isolated c rings (lanes 3, 4 and
7) were boiled ... NaCl and 3 mm NaN
3
for 24 h at 25 °C and
another 24 h at 37 ° C. The 2D crystals were stored at 4 °C
until further analysis.
Atomic force microscopy
An atomic force microscope (Nanos...