... important portion of the fusion protein wasdegraded by the host proteases. On the other hand,attempts to produce a His-tagged version of the Rv1771 protein by using the pRSETc or the GatewaypDEST17 ... confirmed by enterokinasetreatment. After digestion of the affinity-purified GST-tagged protein with enterokinase followed by concen-tration, the 70 kDa band disappeared, and two newbands of 45 and ... oxidoreductases.In order to determine the enzymatic activity of the GST-tagged Rv1771 protein of M. tuberculosis, the oxidase activity was tested in the presence of the l-gul-ono-1,4-lactone and l-galactono-1,4-lactone...