Báo cáo khoa học: Versatile regulation of multisite protein phosphorylation by the order of phosphate processing and protein–protein interactions pptx

... RL & London IM (1984) The regulation of initia- tion of protein synthesis by phosphorylation of eIF- 2(alpha) and the role of reversing factor in the recycling of eIF-2. J Biol Chem 259, ... 2007) doi:10.1111/j.1742-4658.2007.06110.x Phosphorylation of the a-subunit of the eukaryotic initiation factor 2 (eIF2) on Ser51 is an early event associated with...

Ngày tải lên: 07/03/2014, 05:20

... cases. The order of phos- phate processing and the characteristics of protein protein interactions turn out to be of overriding importance for both sensitivity and speed of response. Random phosphate ... thermodynamic aspects of complex multisite phosphorylation on the one hand, and the specific and versatile regulation of protein activation requ...

Ngày tải lên: 07/03/2014, 10:20

... composed of a hOrn and a haOrn moiety. Furthermore, a long-range 1 H– 13 C correlation was detected between the carbonyl carbon of the serine and the d-CH 2 of the hOrn, which constitutes the DKP. Therefore, ... characterization of these compounds is growing and may result in the synthesis of specific inhibitors based on the structure of the pathogen sideropho...

Ngày tải lên: 16/02/2014, 09:20

... MS data on the components of all HPLC fractions indicate the presence of a variety of lipid, sugar and phosphate Fig. 1. The young of Macropus eugenii (A) climbing into the pouch and (B) attached ... (i.e. when the young has no immune system of its own), when there is a profound fall in the microbial flora of the pouch [6]. Neither eugenin nor other low molecula...

Ngày tải lên: 19/02/2014, 16:20

... show that the data fit well with the indi- vidual determination of flux control coefficients by PFK, PK and LDH. The role of the las operon is dis- cussed on the basis of the distribution of flux control for ... the activity of the PTS system and thereby result in further increases in hexose phosphate pools. The decreased LDH activity may contribute further to...

Ngày tải lên: 19/02/2014, 17:20

... preparation. Of particular importance is the finding that exogenous cADPR failed to increase the spontaneous release of ATP in the absence of CD38. In other words, the presence of CD38 is mandatory for the ... nucleotides and nucleosides plus products of the degradation of the exogenous cADPR, and therefore were not analyzed in detail. The enhancing effect of...

Ngày tải lên: 05/03/2014, 23:20

... important portion of the fusion protein was degraded by the host proteases. On the other hand, attempts to produce a His-tagged version of the Rv1771 protein by using the pRSETc or the Gateway pDEST17 ... confirmed by enterokinase treatment. After digestion of the affinity-purified GST- tagged protein with enterokinase followed by concen- tration, the 70 kDa b...

Ngày tải lên: 07/03/2014, 12:20

... Gla(3)–TxVI: confirmation of the presence of BrTrp. Product ion mass spectrum of the doubly charged ion at m ⁄ z ¼ 660.18. The isotopic distribution of the b 2 ion (inset) indicates the presence of bromine. The MS ... to the N-terminal signal peptide [7,14– 17]. The propeptide mediates binding of the substrate to the carboxylase and also activates the enzyme...

Ngày tải lên: 23/03/2014, 11:20

... high-resolution images of protein localization and protein protein interactions [55] Isothermal titration calorimetry Measure thermodynamic constants, affinity and stoichiometry of a protein protein interaction [28] Analytical ... methods. These approaches offer the opportunity to probe the basic mechanism of protein protein interactions in great detail. First, the...

Ngày tải lên: 23/03/2014, 15:21

... Effects of different concentrations of CsA and its analogs on the Ca 2+ -dependent induction of opening of the MPTP. The data are representative of four experiments. A concentration of 1 m M Ca 2+ was ... from the mito- chondrial matrix towards the inner membrane space, thereby creating a proton gradient. The energy stored in the proton gradient is used to drive t...

Ngày tải lên: 30/03/2014, 08:20