Báo cáo khoa học: Acidic extracellular pH increases calcium influx-triggered phospholipase D activity along with acidic sphingomyelinase activation to induce matrix metalloproteinase-9 expression in mouse metastatic melanoma pot

... 3183 Acidic extracellular pH increases calcium in ux-triggered phospholipase D activity along with acidic sphingomyelinase activation to induce matrix metalloproteinase-9 expression in mouse metastatic melanoma Yasumasa ... attenu- ated matrix metalloproteinase-9 induction but did not affect calcium in ux. We found that acidic sphingomy...

Ngày tải lên: 07/03/2014, 09:20

... resolved. To ensure that the flotation was due to binding to light vesicles, 1% Triton X-100 and 1% SDS were added to dissolve the membranes, as demonstrated for BIP, synaptophysin and biotinylated ... p25a p25aDN, p25aDC and the p25a core, corre- sponding to residues 44–156, were cloned into pET1 1d vector, expressed in E. coli and purified. The purified proteins were subjected to...

Ngày tải lên: 14/02/2014, 21:20

... aB-crystallin. Reduction-induced aggregation of the insulin B-chain Light scattering due to DTT-induced amorphous aggregation and precipitation of the insulin B-chain commenced after 10 min and reached ... aB-crystallin protects against the DTT-induced aggregation of insulin, and this activity is enhanced by Arg-HCl. Insulin ( , 0.25 mgÆmL )1 ) was incubated at 37 °C in 50 m M phosphate...

Ngày tải lên: 18/02/2014, 16:20

... Preincuba- tion of CatE with the antibody showed a dose-depend- ent inhibition of antibody binding (IC 50 ¼ 48.6 ng; Fig. 2C). Increasing concentrations of CatD did not affect antibody binding. This experiment ... CatD is reported to be involved in processing MHC II-associated invariant chain [24] in antigen processing and presentation [25,26]. CatE is also reported to be involved...

Ngày tải lên: 07/03/2014, 09:20

... this involves storing energy as fat during periods of an abundant food supply to hedge against periods of food shortage. Today, humans have pushed storage too far, to the point of widespread obesity. Although ... preferable to starvation, this state frequently leads directly or indirectly to serious pathol- ogies including diabetes and heart disease. Interven- tions to diminish adip...

Ngày tải lên: 14/02/2014, 22:20

... stud- ies, the human control and DRPLA brain tissues were fixed in 10% formalin and embedded in paraffin. The sections from the brain tissues were immunostained with ATN1 antibodies as described ... and Gerontology, Aichi, Japan Introduction The polyglutamine (polyQ) diseases are a group of hereditary neurodegenerative disorders that include Huntington’s disease (HD), dentatorubral-pallid...

Ngày tải lên: 15/02/2014, 01:20

... locali- zation within the developing neural tube [3,62,121]. In addition, mHSF2 is associated with DNA binding in the developing brain, including cortex, striatum, olfactory bulbs and mesencephalon, ... before and after birth in mouse brains [122,124]. No HSF4 DNA-binding activity was reported in the developing mouse brain so far. How- ever, Hsf4b is expressed in the adult...

Ngày tải lên: 18/02/2014, 04:20

... its inhibitory effects at or before the adipogenic commitment stage and that the IDM-induced genes appear to overcome the inhibitory effects of miR-27. In order to determine whether miR-27 inhibits ... +IDM +IDM A B C D Fig. 4. Inhibition of expression of PPARc and C ⁄ EBPa in preadipocytes by miR-27 . (A) 3T3-L1 preadipocytes were transfected with miRNAs, and then induced...

Ngày tải lên: 18/02/2014, 08:20

... replaced by Cys in order to introduce a symmetry-related disulfide bond with the Cys residue at position 13 of the other sub- unit [25,26], yielding a covalently bridged dimer. The oxidized and reduced ... contained 100 mm triethanol- amine, 5 mm EDTA, 0 .5 mm NADH and a-glycerolphosphate dehydrogenase (20 lgÆmL )1 ) and 0.10–3.0 mm glyceralde- hyde 3-phosphate. Enzyme activity was det...

Ngày tải lên: 18/02/2014, 11:20

... stressed the importance of light-induced protein degradation in phytochrome-con- trolled signalling and showed data on mutants, isolated in a custom-designed genetic screen, that are impaired in ... showed that the CUL4–DDB1– DDB2 complex plays a role in nucleotide excision repair of UV-C-induced DNA damage and that this activity is controlled by the ATR kinase [68]. In addition,...

Ngày tải lên: 18/02/2014, 11:20