Báo cáo khoa học: Characterization of the NADH:ubiquinone oxidoreductase (complex I) in the trypanosomatid Phytomonas serpens (Kinetoplastida) ppt

... Characterization of the NADH:ubiquinone oxidoreductase (complex I) in the trypanosomatid Phytomonas serpens (Kinetoplastida) Petra C˘erma´kova´1, ... oligomer of the small form. The different in uences of DPI on the two forms could be caused by its failure to access the ﬂavin cofactor in the large form of complex I,whereas the cofactor remains ... arrows).Owing to the horizontal smear, however, the presence of the B14 subunit in the lower band cannot be ascer-tained at this point. A cross-reaction of the antibodyagainst the 39 kDa subunit of...

Tài liệu Báo cáo khoa học: Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. ppt

... N-terminalresidues of the catalytic domain are under-lined. The preregion is indicated in red, the pro-region in black, catalytic domain in blueand the C-terminal domains in violet. The assumed ... One or more of the disulﬁde bondsis shown to be essential for maintaining the activeconformation of VPRK, since cleavage of the disul-ﬁdes lead to inactivation of the enzyme [25]. The ﬁrst two ... extended C-terminalregion(s) of the peptidase sequences are commonwithin the bacterial subgroup of the proteinase K fam-ily. Database search on the second C-terminal domain(CII) of SPRK revealed...

Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

... wasbased on the conserved amino acid sequences of the catalytic domain of members of the GH18 familyresulted in the ampliﬁcation of an expected 147 bpsequence. Cloning and sequencing of this fragmentrevealed ... of the soleGlyco_18 domain, the C-terminal tail of C. gigas CLPsmay not noticeably contribute to the structure and the function of these proteins. Interestingly, Cg-Clp1 andCg-Clp2 C-terminal ... the oyster Crassostrea gigas [5]. Interestingly, thisprotein, named C. gigas chitinase-like protein 1 (Cg-Clp1) was found to be involved in the control of growth and remodelling processes in...

Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

... of alternative promoters in DNASE1 results in no generation of protein isoforms. The 5¢-UTR of eukaryotic mRNA in uences the initi-ation step of protein synthesis and thereby in partdetermines ... expression in vivo or in vitro, including characterization of the promoterregion of the gene and the associated transcriptionalfactors. Therefore, delineation of the transcriptionalregulation of human ... calculated using the sequence detection software as the cycle number atwhich the ﬂuorescence of the reporter dye crossed the threshold in log-linear range of PCR. The copy numbers of the respective...

Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... or byFC-induced binding of 14-3-3s.Results The C-terminus of isoform 1 of the PM H+-ATPase of A. thaliana (AHA1) interacts with the ﬁrst 88 aminoacids of PPI1 [22], indicating that the PM ... suggested by the parallel increase of the number of FC-bindingsites [18–20]. As to auxin, a soluble auxin receptor hasbeen reported to bind and activate the PM H+-ATPase,but the site of binding has ... fusicoccin-induced binding of 14-3-3 proteins. The latter treatment did not affectPPI1 afﬁnity for the H+-ATPase. These results indicate that PPI1 can bind the H+-ATPase independently of the...

Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx

... of the genes. In SNO1, the 199th G from the 5¢ terminus of the open readingframe was deleted to result in a frame-shift and appearance of stop codons in the downstream. As a result, a protein ... acetylpyridine adenine dinu-cleotide (APAD) as cosubstrate [17]. The glutaminasereaction was carried out in 0.3 mL of 50 mMTris/HCl,pH 8.0, in the presence of 1–10 mMglutamine and 30 lgof the ... the skeleton of pyridoxine in yeast[9,10]. Herein, we report that Sno1p and Snz1p serve as aglutaminase to supply ammonia for the ring nitrogen of pyridoxine in yeast. Based on these and other...

Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx

... and the novel Prm3 [25] relative to itstranslational start site (ATG, designated +1) ispresented in Fig. 1. In order to gain further insightsinto the modes of regulation of TPb, the aim of the current ... Transcriptional activation of the human hemato-poietic prostaglandin D synthase gene in megakaryo-blastic cells. Roles of the oct-1 element in the 5¢-ﬂanking region and the AP-2 element in the untranslated ... of Oct-2,but not Oct-1, in HEL cells and supershift assaysemploying anti-(Oct-2) IgG further conﬁrmed Oct-2binding to the Oct-1 ⁄ 2 site of Prm3. Owing to the absence of Oct-1 expression in...

Tài liệu Báo cáo khoa học: Characterization of ICAM-4 binding to the I domains of the CD11a/CD18 and CD11b/CD18 leukocyte integrins pptx

... localize the ligandbinding sites in the leukocyte b2integrins. The a chains of the CD11/CD18 integrins contain an inserted approxi-mately 200-amino-acid intervening domain (I domain),which ... anti-ICAM-4antibodies, indicate a major role for ICAM-4 in binding of red cells to the I domains. The efﬁcient inhibition of the Fig. 10. Speciﬁc binding of puriﬁed recombinant I domain GST fusionproteins to ... degrees of inhibition weredetected. The most efﬁcient inhibition of the binding of ICAM-1 L cell transfectants to the I domains wasobtained with the ICAM-1 mAb LB-2 reacting with the ﬁrst domain of...

Tài liệu Báo cáo khoa học: Characterization of the promoter for the mouse a3 integrin gene Involvement of the Ets-family of transcription factors in the promoter activity doc

... binding factors. The introduction of mutation in one of the Ets-bindingsequences greatly decreased its promoter activity, suggestingthat the transcription of the a3 integrin gene in these cells ... includingEts, GATA, and MyoD/E-box binding factors. The introduction of mutation into one of the putative Ets-binding sequences suppressed the promoter activity. In addition, the speciﬁc binding ... PCR products in the absence of the cDNA library.Fig. 6. Transcription start sites in the integrin a subunit genes. The sequences ﬂanking the transcription start sites in the integrin a subunitgenes...

Báo cáo khoa học: Characterization of the rice carotenoid cleavage dioxygenase 1 reveals a novel route for geranial biosynthesis ppt

... suggested a single cut at the C9–C10 double bond of the ring-bearing side of 3-OH-c-carotene in combination with several cleavageoptions in the linear half of the molecule, namely at the C9¢–C10¢,C7¢–C8¢ ... temperature of 340 °C, which was maintained for an addi-tional 5 min. The He carrier gas ﬂow was maintained at1mLÆmin)1using a split ﬂow of 1 : 20. The splitless timewas 3 min, and the injector ... represent ratios of the C17and C19dialdehydes in the sum of their peak areas calculated by integratingeach peak at its individual kmax. Data represent the average of sixindependent incubations.Fig....

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