Báo cáo khoa học: Inhibition of an iron-responsive element/iron regulatory protein-1 complex by ATP binding and hydrolysis docx

... regulatory protein-1 complex by ATP binding and hydrolysis Zvezdana Popovic and Douglas M. Templeton Laboratory Medicine and Pathobiology, University of Toronto, Canada Mechanisms of post-transcriptional ... (GraphPad Software, San Diego, CA). Acknowledgements This work was supported by grants from the Heart and Stroke Foundation of Canada (grant T4134) and the...

Ngày tải lên: 07/03/2014, 09:20

... interaction between ETA and cyto- solic EF-2 on the one hand, and the mitochondrial membrane on the other hand. However, the potential role (if any) of ADP-ribosylation of EF-2 in the mitochondrial ... absence of ATP, the substrate of the vacuolar H + -ATPase pump (Fig. 3A). Immunoblot analyses showed a progressive loss of intact ETA and ETA-A in the presence of ATP, with...

Ngày tải lên: 18/02/2014, 04:20

... implication of another class of transcriptional regulator in JH signaling. Wilson and coworkers examined the resistance mechanism of Dro- sophila to the JHA, methoprene, and isolated mutant lines of flies ... having overhangs of EcoRV and XbaI sites was prepared by PCR and subcloned into pBIND vector (Promega); the cDNA fragment containing the fused ORF of GAL4–DBD and...

Ngày tải lên: 16/03/2014, 18:20

... Shi Q, Zhang Z, Li G, Pillow PC, Hernandez LM, Spitz MR & Wei Q (2005) Polymorphisms of methionine synthase and methionine synthase reductase and risk of lung cancer: a case-control analysis. ... reactions of trans-sulfura- tion through the production of homocysteine, biologi- cal methylations of DNA, lipids and proteins, and polyamine biosynthesis through the production...

Ngày tải lên: 19/02/2014, 05:20

... Con- formational changes induced by ligands on C-LytA monitored by near-UV CD with no ligand added (—) and upon addition of ligands: s, d, 2.5 and 20 m M choline; n, m, 2.5 mM and 20 mM atropine; h, ... other hand, ipratropium and atropine also induced the appearance of these negative bands, although with reduced intensi- ties and with a much lower concentration of ligand,...

Ngày tải lên: 19/02/2014, 05:20

... [51,52]. The O of Ser90 and the S of Cys266 of pea FNR are close to N5 and O4 of the isoalloxazine, which are involved in hydride transfer. The hydroxy group of Ser90 could accept a hydrogen bond and thus ... (0, 1.5, 3 and 5 l M ), at different concentrations of NADPH (5, 10, 25, 50, 100 and 300 l M ), DCPIP (5, 10, 15, 25, 35 and 50 l M ), ferricyanide (100, 250, 500...

Ngày tải lên: 19/02/2014, 16:20

... (a-1,3- and a-1,6-glu- can), nigeran (a-1,3- and a-1,4-glucan), soluble starch (a-1,4- and a-1,6-glucan), pustulan (b-1,6-glucan), laminarin (b-1,3-glucan), carboxymethilcelullose (b-1,4-glucan) ... resistance in plants [3], their potential for promoting plant growth [4] and their antagonistic activity against a wide range of plant pathogenic fungi [5]. The inhibi- tory effect of...

Ngày tải lên: 19/02/2014, 16:20

... being more sensitive than GR and LDH, and inactivation of G APDH being metal- ion independent, whereas inhibition of GR and LDH by H 2 O 2 occurs most rapidly in the presence of metal ions. It is ... peroxides a re also Table 1. Inhibition of glutathione reductase by H 2 O 2 and N-Ac-Trp-OMe peroxides, and lactate dehydrogenase by H 2 O 2 , in the presence and ab...

Ngày tải lên: 21/02/2014, 03:20

... expression of Akt and PrP C was examined by immunohistochemistry and confocal microscope in human gastric cancer cases and cell lines. Expression of total and p-Akt was detected in cells transfected ... resistance in gastric cancer cells Jie Liang*, Fulin Ge*, Changcun Guo, Guanhong Luo, Xin Wang, Guohong Han, Dexin Zhang, Jianhong Wang, Kai Li, Yanglin Pan, Liping Yao, Zhanxin...

Ngày tải lên: 07/03/2014, 03:20

... of Ca 2+ (lanes 5, 6 and 9, 10). In the absence of Ca 2+ (lanes 3, 4 and 7, 8) and for the GST control (lanes 1 and 2) there was no retention of hCaM detectable. Table 1. Binding of MCGS-(H) 6 -hCaM ... channels by Ca 2+ ⁄ CaM. Employment of CaM-EF-hand mutants showed that CaM binding to the N- and C-ter- minus are primarily dependent on EF-hand motifs 3 and 4. Hen...

Ngày tải lên: 07/03/2014, 12:20