Báo cáo khoa học: Equilibrium distribution of skeletal actin–tropomyosin– troponin states, determined by pyrene–tropomyosin fluorescence potx

... Chalovich Distribution of actin–tropomyosin troponin states FEBS Journal 274 (2007) 2287–2299 ª 2007 The Authors Journal compilation ª 2007 FEBS 2299 Equilibrium distribution of skeletal actin–tropomyosin– troponin ... actin–tropomyosin– troponin states, determined by pyrene–tropomyosin fluorescence Boris Gafurov 1 and Joseph M. Chalovich 2 1 Uniformed Services...

Ngày tải lên: 07/03/2014, 09:20

... the posi- tion of the filter (basolateral side of cells). Scale bar ¼ 6 lm. A B Fig. 5. Expression of iNOS in duodenum tissue of mice. (A) Quanti- fication of iNOS mRNA induction by flagellin in ... distribu- tion of iNOS activity. The enzyme activity was determined by the amount of citrulline produced in cytosol vs. resuspended particulate fraction and was expressed as perce...

Ngày tải lên: 19/02/2014, 16:20

... goal of this work was to investigate the possibility that changes in the expression of gangliosides could modulate the membrane distribution of EGFr members and thereby regulate their function. By ... Glycolipid labelling of CHO-K1 cell clones. Aschematicrep- resentation of the pathway of glycolipid biosynthesis is shown at the top of the figure. It is appreciable how the pat...

Ngày tải lên: 16/03/2014, 16:20

... resulting se- quences are of a length which no practicable machine can manage. A study of the distribu- tion of the number of symbols between pairs of successive symbols of certain classes would ... words. The length distribution of the latter subsequences has the desirable property, not shared by the others, of being concentrated at relatively low values of length...

Ngày tải lên: 16/03/2014, 19:20

... exhibit a wide range of structural diversity produced by a sequence of biosynthetic reactions. Cyclization of oxidosqualene is the ini- tial origin of structural diversity of skeletons in their ... the production of not only flavonoid but also of triterpene saponins is induced by elicitation as mentioned above, triterpene hydroxylase is one possible function of CYP93E1. Fee...

Ngày tải lên: 19/02/2014, 07:20

... half-maximal amounts of pPacUSp1 (50 ng, left panel) or increasing amount of Ets-1 with 50 ng of either Sp1 or Sp3. (B) Assay of fgl2 promoter activity upon cotransfection of thresholds amount of both Sp1 ... site of fgl2 mRNA. (A) 5¢-RACE of fgl2 mRNA from macrophages. Stars indicate the 5¢-ter- minus of 33 clones isolated after 5¢-RACE. (B) Primer extension analysis of f...

Ngày tải lên: 20/02/2014, 11:20

... fdcreina@quim.iq.usp.br Abbreviations: Tn, troponin complex; TnI, skeletal troponin I; 5HW, 5-hydroxytryptophan; TnC, skeletal troponin C; TnT, skeletal troponin T. (Received 10 October 2002, revised ... reconstitution of troponin complexes. TheabilityofeachmutantTnItobindTnCwasassessedbyurea/ PAGEinthepresenceof(A)0.5m M EDTA and (B) 0.5 m M Ca 2+ .In the absence of Ca 2+...

Ngày tải lên: 20/02/2014, 11:20

... activity of W35A compared to that of WT Trx was reduced by a factor of approximately two [25]. T hirdly, a ctivity of D 30A was weakly modified only after irradiation w ith 100 Gy, while that of WT ... selective and provides a simpler model of oxidation than OH  radicals allowing the determination of the main process of o xidation of OH • radicals, without all of the sid...

Ngày tải lên: 07/03/2014, 16:20

... new carboxylesterase has molecular weight of 58 kDa as deter- mined by SDS/PAGE and pI of 6.0 (Table 4). Analysis of the new protein by SCANPROSITE software (http://www. expasy.ch/tools/scnpsit1.html) ... pharmacokinetics of retinyl ester metabolism will be determined by the tissue specific expression of carboxylesterase and their catalytic efficiency for ester hydrolysis. Suc...

Ngày tải lên: 08/03/2014, 10:20

... tandem GAF domain of PDE10, and by cGMP when linked to the tandem GAF domain of PDE2, )5or)11. The bio- chemical properties of signalling by human tandem GAF domains to cyaB1 AC were by and large ... at the beginning of the tandem of upstream conserved regions, which precedes the catalytic domain. PDE4 activation by this phosphorylation is about twofold, i.e. in the same ord...

Ngày tải lên: 16/03/2014, 06:20