Báo cáo khoa học: Protein folding includes oligomerization – examples from the endoplasmic reticulum and cytosol doc

... fam- ily and their co-chaperones, such as the DnaJ proteins. However, once the protein is released from the ribo- some, the folding pathways in the cytosol and ER may well diverge. The ER is ... mediated by the other components of the TCR complex, the de, ce and ff dimers, which assemble step by step with the ab dimer. C. Christis et al. Protein folding...

Ngày tải lên: 07/03/2014, 06:20

... molecules for export of proteins from the ER, and promote transport of newly synthesized proteins from the ER into ER subdomains or Golgi in yeast [16 7–1 70]. Newly synthesized proteins that do not ... development, where the UPR is presumed to enhance the ability of the ER to fold these proteins [195]. However, there is currently no experimental confirmation of this, and...

Ngày tải lên: 16/03/2014, 11:20

... Broad zones with R f 0. 2–0 .26 and 0.6 3– 0.77 containing DGDG and MGDG were scraped from the plates. DGDG and MGDG were eluted from silica with ethanol. The UV spectra of DGDG and MGDG were recorded ... (6.26 p.p.m., dddt) and H4¢¢ ¢ (5,42 p.p.m., m). These signals, their multiplicity, coupling constants and the arrangement of the spin interactions between them...

Ngày tải lên: 23/03/2014, 05:22

... high temperature. The gel shift of 5 kDa is similar to the other modifiable membrane proteins [2 0–2 2]. We assume that the 50 kDa protein is fully folded protein and that the 53 and 55 kDa proteins could ... 0.1 (C m1 )– – 3.2 ± 0.1 (C m2 )– – Fluorescence 1.8 ± 0.1 (C m1 )– – 3.2 ± 0.1 (C m2 )– – D. R. Mendu et al. Protein folding intermediates F...

Ngày tải lên: 07/03/2014, 05:20

... pests, namely the car- boxypeptidase inhibitors from the leech and from the tick, and the leech tryptase inhibitor. Structures of these intermediates have been solved, and the folding path- ways ... their coat proteins, and A. Land (Utr- echt, The Netherlands) described how complex this is Protein folding and disulfide formation A. M. Benham 6906 FEBS Journal 27...

Ngày tải lên: 29/03/2014, 22:21

... (i.e. the outer and inner envelope mem- brane and the photosynthetic thylakoid membranes) as well as three soluble subcompartments, the thylakoid lumen, the stroma and the intermembrane space. The large ... majority of chloroplast proteins are encoded by nuclear genes, synthesized in the cytosol and post- translationally imported into the organelle [1,2]. In the c...

Ngày tải lên: 23/03/2014, 07:20

... into the nucleus and enhances secretory granule gene expres- sion by binding and protecting STAT5 phosphotyro- sines. For the R4 (RPTPa and RPTPe) and R5 (RPTPc and RPTPf) receptor-type PTPs the ... isolation of native protein complexes, for example, by exploiting tandem affinity purification protocols and the selective enrichment of phosphoprotein-containing proteins, an...

Ngày tải lên: 30/03/2014, 04:20

... been employed in protein structure prediction [1 8–2 7], as well as in the prediction of protein protein [25,2 8–3 0] and protein ligand interactions [3 0–3 3]. A few studies have explored the use of knowledge-based ... better than the reduced atom approach. The protein RNA score has distinctive properties compared to the protein DNA potential. When we scored the p...

Ngày tải lên: 18/02/2014, 16:20

... reference, the position of the sugar and of the metal in the crystallo- graphic template 1YOE. The two ligands were modelled in each binding site of SsCU-NH, and then the structure of the resulting ... of the program, setting the highest level of optimization. The quality of the mod- els and their stereochemical properties were analyzed using the programs prochec...

Ngày tải lên: 18/02/2014, 17:20

... space as did the selection for proper folding of the initially randomized helices with the wild type template. A sequential strategy of randomizing helix 1 first and then randomizing helices 2 and 3 ... (Fig. 3): first, the N-terminal helix alone was randomized, secondly, the two C-terminal helices were randomized simultaneously, and finally, positives from the first two lib...

Ngày tải lên: 19/02/2014, 12:20