Báo cáo khoa học: Characterization of membrane-bound prolyl endopeptidase from brain ppt

... purification and characterization of isoforms of an extracellular serine glutamyl endopeptidase of Enterococcus faecalis with different enzymatic activities. J Bacteriol 187, 266–275. 30 Pavloff N, Pemberton ... presence of SDS. Role of N-terminal Val69 in processing of the GluV8 proform Finally, the role of N-terminal Val69 of mature GluV8 was investigated. It has been pro...

Ngày tải lên: 16/03/2014, 06:20

... and DcrH-Hr. Dv: DcrH-Hr fragment of DcrH from D. vulgaris. Mc: McHr of M. capsulatus. AB Fig. 7. SDS ⁄ PAGE analysis of proteins during purification of McHr. Samples from each step in the purification ... Lillehaug Department of Molecular Biology, University of Bergen, Norway Haemerythrin proteins comprise a family of O 2 -carrry- ing proteins mainly found in a few phyla of...

Ngày tải lên: 07/03/2014, 17:20

... biochemical characterization of a c-tocopherol methyltransferase (c-TMT) from Arabidopsis thaliana and of a c-TMT purified from Capsicum annuum fruits, a tissue with high accumulation of tocopherols. ... nutritional quality of crop plants [6]. Biosynthesis of tocopherols was demonstrated in plastid envelopes [7] from precursors originating from the plastidial isoprenoid pat...

Ngày tải lên: 17/03/2014, 09:20

... al.[12]. Purification and characterization of carboxypeptidases from H. armigera larval gut extract Gut extract was prepared from fourth instar lavae of H. armigera as described previously [10]. Extract from 65 larvae ... and characterization of cDNAs encoding H. armigera carboxypeptidase The construction of a cDNA library in the phage k vector Lambda Uni-ZAP XR (Stratagene)...

Ngày tải lên: 23/03/2014, 12:20

... al. Membrane-bound prolyl oligopeptidase: mPOP FEBS Journal 275 (2008) 4415–4427 ª 2008 The Authors Journal compilation ª 2008 FEBS 4427 Characterization of membrane-bound prolyl endopeptidase from ... The amounts of protein loaded onto gels were as follows: for the PSD- 95 blot, 70 lg of TM, 96 lg of LM, 89 lg of MM, and 93 lgof HM; for the calnexin blot, 70 lg of T...

Ngày tải lên: 07/03/2014, 06:20

... 10 6 mL )1 . Next, 0.5 mL of propidium iodide staining solution (10 mg of propidium iodide, 0.1 mL of Triton-X, 3.7 mg of EDTA in 100 mL of NaCl ⁄ P i ) was added to 0.5 mL of cell suspension. The ... spectra of extracted lipids (A) and PCA extracts (A¢) from one representative sample of MCF-7 cells; 2D 1 H NMR spectra of extracted lipids (B) and PCA extracts (B¢) from o...

Ngày tải lên: 18/02/2014, 13:20

... gigas. In this article, we report the characterization of a new CLP, named Cg-Clp2, from the oyster C. gigas. The tissue distribution and temporal pattern of expres- sion of the gene encoding Cg-Clp2 ... conserved amino acid sequences of the catalytic domain of members of the GH18 family resulted in the amplification of an expected 147 bp sequence. Cloning and sequencing of...

Ngày tải lên: 19/02/2014, 00:20

... number of transcripts containing exon 1a with the number starting from exon 1. The abun- dance of transcripts starting from exon 1 was 10-fold higher than that of the transcripts starting from exon ... 5¢-flanking region of exon 1 in DNASE1. Deletion of the upstream end of the 5¢-flanking region of exon 1 from position )1386 to )231, )116, or )78 did not result in any sign...

Ngày tải lên: 19/02/2014, 06:20

... are from one experiment, representative of three giving similar results. Fig. 6. Dependence on the concentration of PPI1 588 His 6 of the sti- mulation of H + -ATPase activity in PM purified from ... the mechanism of action of PPI1. The analysis of the pH dependence of PPI1- induced activation of the H + -ATPase showed that stimulation decreases dramatically with the incr...

Ngày tải lên: 19/02/2014, 07:20

... Proteinase K from Tritirachium album Limber. Characterization of the chromosomal gene and expression of the cDNA in Escherichia coli. Eur J Biochem 179, 185–194. Characterization of a Serratia ... Isolation of genomic DNA from Serratia sp. Genomic DNA was isolated as described by Chen and Kuo [38] for use in identification of the peptidase gene. Generation of an  200-bp fr...

Ngày tải lên: 19/02/2014, 07:20