Báo cáo khoa học: Structural mobility of the monomeric C-terminal domain of the HIV-1 capsid protein pptx

... at the entrance of the cleft of EcoMBP, but the distance between Od2 of Asp209 and Ng2 of Arg344 is only 10.2 A ˚ .InTliTMBP, the cleft is the smallest among the three proteins (Fig. 7C). The ... half-buried, and the form of the cleft is markedly different from those of TvuCMBP and EcoMBP. These observations suggest that the structure of the N -domain part...

Ngày tải lên: 19/02/2014, 00:20

... respectively, of that of HPRT. This low activity could be explained by the fact that PRTFDC1 has a Gly in the position of the proposed catalytic Asp of HPRT. In PRTFDC1, a water molecule at the position of ... strength [20]. In the present study, we report the structural and functional characterization of the human HPRT homolog PRTFDC1. To shed light on the poten...

Ngày tải lên: 15/02/2014, 01:20

... hinge domains The composition of the two hinges that connect the FMN subdomain in NOS enzymes (H1 and H2 in Fig. 5) defines the allowable movements of the FMN subdomain and thus controls the FMN–NOSoxy ... in NOS, the second electron is provided to the heme- dioxy species by a bound H 4 B cofactor rather than by the flavoprotein domain [16]. The H 4 B radical is then r...

Ngày tải lên: 18/02/2014, 11:20

... nature. The growing body of sequence and structural data on these well-studied enzymes affords an opportunity to evaluate the conse- quences of mutations. In the case of TIM, only nine of the 220–250 ... method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72, 248–254. 54 Oesper P & Meyer...

Ngày tải lên: 18/02/2014, 11:20

... evidence supporting a structural interruption of the geometry of the recognition helix for either Antp or the TTF-1 HD, the anomalous amide exchange pattern and the NOE connectivity data of the C-terminal ... conflict with the global interpretation of the data. With the exclusion of the N-terminal octapeptidyl and C-terminal nonapept- idyl fragments of Glu...

Ngày tải lên: 18/02/2014, 16:20

... proton from the catalytic Cys pres- ent at the N-terminus of the helix to the His of the dyad [19,50,51]. The first stage of catalysis is mediated by the highly active thiolate ion of the Cys. Biochemi- cal ... erva- tamin-A, the P3 moiety of both the molecules of the asymmetric unit runs along the extended backbone of residues 65–64 and is partly exposed...

Ngày tải lên: 18/02/2014, 16:20

... urchin EGF-like protein and bone morphogenic protein 1 [16]. These proteins are often referred to as the prototype CUB domains. Like the prototype CUB domains, the PDGF-C and -D CUB domains span ... the prototypic CUB domains, respectively (Fig. 2) [8]. The CUB domains of PDGF-C and -D share  55% sequence identity. CUB domains are assembled as a Fig. 2. The CUB domain. Th...

Ngày tải lên: 19/02/2014, 07:20

... motif’, a structurally uncharacterized motif. Fig. 2. An overview of the PhKc domain structure. The first 296 residues of the subunit encode a protein kinase domain. The struc- ture of the constitutively ... these structures a C-terminal extension to the protein kinase folds back on the kinase domain and interferes with the substrate binding sites. In the cas...

Ngày tải lên: 19/02/2014, 17:20

... about the function of the protein. The Fig. 1. (A) Cartoon representation of CagZ protein fold. The L-shape is clearly visible. (B) Two views of the electrostatic potential surface of CagZ. In the ... side of the peanut. The results of the crystallo- graphic model of CagS do not show any clear evidence of architectural similarity to other known structures,...

Ngày tải lên: 06/03/2014, 00:21

... C-lobe domain containing 1) [9]. The KIND domain in these proteins is localized to the N-terminal region, and their specific functional domains are located in the C-termi- nal region. The C-lobe of protein ... central domain of MAP2 contain the v-KIND binding core module MAP2 consists of three main structural domains: the cAMP-dependent protein kinase regulatory s...

Ngày tải lên: 14/02/2014, 19:20