Báo cáo khoa học: Insights into substrate and product traffic in the Drosophila melanogaster acetylcholinesterase active site gorge by enlarging a back channel ppt

... Insights into substrate and product traffic in the Drosophila melanogaster acetylcholinesterase active site gorge by enlarging a back channel Florian Nachon 1 , Jure Stojan 2 and Didier ... hydrolysis because the traffic can pass via an alternative route, presumably the enlarged back channel. Abbreviations CAS, acylation site; DmAChE, Drosophi...

Ngày tải lên: 07/03/2014, 05:20

... enhance- ment of the 57-kDa band with a concomitant diminu- tion of the 38-kDa and 19-kDa bands, as analyzed by SDS ⁄ PAGE (Fig. 3A) . The 38-kDa and 19-kDa bands did not disappear completely after ... proteinase. Structure 10, 865–876. 7 Wlodawer A, Li M, Gustchina A, Tsuruoka N, Ashida M, Minakata H, Oyama H, Oda K, Nishino T & Nakayama T (2004) Crystallographic and bi...

Ngày tải lên: 19/02/2014, 07:20

... agmatinase activity of the Asn149Asp vari- ant, it is clear that the interactions of arginase II with l-arginine and agmatine are greatly altered by replace- ment of this residue with aspartate. ... 5¢-CTGGGAGGAGA CAACAGCCTGGCAATC-3¢ for His120Asn, 5¢-TGGGTT GATGCCAATGCTGACATCAAC-3¢ for His145Asn and 5¢-TGACATCGACACACCCC-3¢ for Asn149Asp. Fluorescence spectra and thermal inactivat...

Ngày tải lên: 20/02/2014, 02:21

... N-terminal to the unpaired cysteinyl residues observed in jararhagin and catrocollastatin. Jarahagin and catrocollastatin are known to undergo post-translational processing to yield a disintegrin-like ... pairs located in the center and in the C-terminal region of the domains are shared between the disinte- grin flavoridin and VAP1. The disulfide bond patterns of th...

Ngày tải lên: 18/02/2014, 18:20

... members of the PLD superfamily. However, there is low similar- ity in the remaining parts of the molecules. Most mam- malian PLDs contain a PX and a PH domain instead of the C2 domain in plants, whereas ... latter being dominant. The fluorescence and near-UV CD spectra point to tight packing of the aromatic residues in the core of the protein. From the near-UV C...

Ngày tải lên: 19/02/2014, 02:20

... put before the publisher and talked [to him] _ROOT_ us__lekhakiiy__asmitaa__ko ham sagarv prakaashak__ke−saamane rakhakar baat__karate_ _the b) a) _ROOT_ isake__baad vah jamaan__shaah aur−phir 1795__meM ... iden- tifying appropriate karakas. The dependency tagset in the annotation scheme has 28 relations in it. These include six basic karaka relations (adhikarana [location], ap...

Ngày tải lên: 20/02/2014, 09:20

... obtain total chemiluminescence. Statistical analysis The animals were maintained at an ambient temperature of 22 °C for at least 90 min to obtain the thermal balance before any tested agent was ... Colavita AR, FitzGerald GA & Violi F (1997) Platelet activation by superoxide anion and hydroxyl radicals intrinsically generated by platelets that had undergone anoxia and then reo...

Ngày tải lên: 19/02/2014, 00:20

... Bofill R, Palacios O, Capdevila M, Cols N, Gonzalez- Duarte R, Atrian S & Gonzalez-Duarte P (1999) A new insight into the Ag+ and Cu+ binding sites in the met- allothionein beta domain. J Inorg ... using the program dyana and the published solution structure of the respective cad- mium-containing domain of the intact protein as a starting point. The resulting stru...

Ngày tải lên: 07/03/2014, 09:20

... tyrosine kinase activity. Domains within Src kinases target the enzyme to specific subcellular locations where they bind to regulatory and ⁄ or substrate proteins and are integrated into cell signalling ... with phosphorylated Tyr527 in the C-terminal tail binding to the SH2 domain. The ligand binding surfaces of the SH2 and SH3 domains are tucked inside, thus present- in...

Ngày tải lên: 07/03/2014, 12:20

... The gel was then dried and auto- radiographed. In the UV cross-linking assay, the samples were incuba- ted at room temperature, as described above for the EMSA assay, and then irradiated at 302 ... morphogenesis, a sixfold elevation in eEF 1A specific activity is accompanied by a dramatic increase in protein methylation at as many as nine lysine residues, without any c...

Ngày tải lên: 21/02/2014, 00:20