... the two CYP17 isoforms were not the product of two alleles of the same gene, but two separate CYP17 genes encoding the two unique CYP17 isoforms. Thisnovel finding was subsequently confirmed by ... encoded by a single gene, two sequences, which differ by two nucleotides, havebeen deposited in GenBank. To date it is unknownwhether these sequences are two alleles of CYP17 orthe result of a ... CA, USA) [20]. Cotransfections of CYP17 and3bHSD, with and without cytochrome b5, were performed with an equal amount of each construct up to a total of 0.5 lg of plasmid DNA using Genejuice...