Báo cáo khoa học: Kinetic properties of catecholoxidase activity of tarantula hemocyanin pot

... the higher inhibitory activity of the cEL peptide and the very low inhibitory activity of the cYT peptide compared to other peptides. The flanking residue of the b-turn, i.e. Tyr3 of the cVY peptide ... chain topologies of the turn region mimic those of rat CD2. Structure of cyclic hexapeptides – cEL peptide. The chemical shifts of amide resonances of peptide cEL were wel...

Ngày tải lên: 19/02/2014, 13:20

... instead of glycopeptides [8]. For detection of IgE reactivity, streptavidin conjugated with horseradish peroxidase instead of alkaline phosphatase was used. After visualization of the enzymatic activity ... subtraction of the spontaneous release of the basophils, the allergen- induced histamine release was calculated as percent of the total amount of histamine determined aft...

Ngày tải lên: 21/02/2014, 00:20

... added. Endotoxin activity determination by the chromogenic Limulus test Endotoxin activity of the glycolipids was determined by a quantitative kinetic assay [27] based on the reactivity of Gram-negative ... of the horseshoe crab Limulus polyphemus,to form a solid gel in the presence of minute amounts of endotoxins. The comparison of LPS and MfGl-II in the LAL assay shows, as...

Ngày tải lên: 21/02/2014, 00:20

... rotation of the bTD module at the main chain of D606 to dock it against the calf-2 domain with its C-terminus in prox- imity of the N-terminus of the b 3 ‘open’ TM helix. Overall evaluation of the ... (involving the swing-out of the b 3 hybrid domain [6]) taking place without the requirement of full separation of the TM helices. Since then, crystallographic structures of the...

Ngày tải lên: 18/02/2014, 04:20

... AAA activity of BuChE in plasma and tissues could participate in the metabo- lism of these aryl acylamide drugs and xenobiotics. However, the potential detoxification role of the AAA activity of ... effects of these compounds on the AAA and esterase activities of human BuChE. The concentrations of tyramine and serotonin that activate or inhibit the AAA activity of BuChE (...

Ngày tải lên: 18/02/2014, 17:20

... constant of the 5¢ exon mimic, h is the fraction of 5¢ exon mimic bound to the ribozyme, and [ribozyme] u is the concentration of unbound ribozyme in the reaction. Measurement of rate constant of substrate-cleavage ... property of nuclear group I introns. RNA 9, 1464–1475. 6 Herschlag D & Cech TR (1990) Catalysis of RNA cleavage by the Tetrahymena thermophila ribozyme. 1. K...

Ngày tải lên: 18/02/2014, 18:20

... FEBS potential regulates lateral distribution of K-Ras after it binds to membrane. The third component of membrane potential, elec- trostatic membrane surface potential, is a consequence of incomplete ... of cytoplasmic composition of anionic li- pids in membrane binding of K-Ras was evaluated by studying the effect of ATP depletion, which inhibits inward movement of PS, with...

Ngày tải lên: 19/02/2014, 00:20

... a twofold molar excess of papain. The residual activity of papain was measured with Z-FR-AMC. Rel- ative activity of cystatin F is shown, from 0% (uninhibited enzyme) to 100% (the lowest activity ... localization of cystatin F, its restricted and readily regulated expres- sion [18], selective and not too potent inhibition of cathepsins are all in favor of the active role of...

Ngày tải lên: 19/02/2014, 17:20

... determine the kinetic para- meters of chitinase A. Having established confidence in the validity of the method, we systematically investigated, by using ESI MS, the kinetic properties of chitinase ... quantities of protein util- izing the principle of protein-dye binding. Anal Biochem 72, 248–254. 33 Laemmli UK (1970) Cleavage of structural proteins dur- ing the assembly of...

Ngày tải lên: 20/02/2014, 01:20

... and absence of 4 lm ChlH; this concentration of ChlH gives almost maximal stimulation of methyltrans- ferase activity. 0.2 lm ChlM was assayed with 1 mm SAM and various concentrations of MgD. Figure ... the methyl carbon of SAM, or the enhancement of the negative charge on the propionate carboxyl groups of MgD. The rate constants quoted in Scheme 1 are all Table 1. Summary of...

Ngày tải lên: 20/02/2014, 02:21