Báo cáo khoa học: Conserved residues in the N-domain of the AAA+ chaperone ClpA regulate substrate recognition and unfolding pdf

... the substrate via two elements in ClpA, one in the N-domain and the other in the pore of the ClpA hexamer. In the case of short unstructured peptides or unfolded proteins such as casein, binding ... have analysed the role of five highly conserved basic residues in the N-domain of ClpA by monitor- ing the binding, unfolding and degradatio...

Ngày tải lên: 07/03/2014, 05:20

... atalytic function of the C-terminal domain is not apparent [12], and thus the role of the corresponding part of the gene is also unknown. These g ene f usions togeth er with single-copy genes of APXs and CCPs ... conserved arginine (Arg622 in HalomarCPc) in t he corresponding position, indicating that these domains lost their ability to co-ordinate the heme. Furthe...

Ngày tải lên: 19/02/2014, 16:20

... amino acid biosynthesis. Much of the generation of NADH during amino acid biosyn- thesis takes place in the mitochondria. Because of the block in the respiratory chain caused by the addition of ... N 2 (containing less than 3 p.p.m. O 2 ) at a flow rate of 0.1 LÆmin )1 per liter of medium, and a stir rate of 500 r.p.m. was maintained. In order to minimize the...

Ngày tải lên: 19/02/2014, 02:20

... using the main chain of residues Ser12 fiThr70 in t he structure a lignment. (B) O verlap of the X-ray structure with the 30 °C NMR structure using the main chain of residues Ser12fiThr70 in the ... considerable number of questions remain before we can solve the interaction at the atomic level and understand t he role of the threonine side chains in ice bin...

Ngày tải lên: 19/02/2014, 16:20

... we then analyzed the genomic organization of the murine o-macs gene. The mouse o-macs is located in the 7F1 region of chromosome 7 and is composed of 14 exons including the 5¢ -and3 ¢-non coding ... guanine nucleotide releasing protein ND – Rat IGF binding protein 5 ND – Rat FGF receptor activating protein 1 ND – Rat PKC delta-binding protein ND – Rat cellular retinoyl-bin...

Ngày tải lên: 08/03/2014, 02:20

... cells. Referring to Smad4, there are significant levels of protein in the nucleus of both cell lines already in the absence of ligand. Cycloheximide treatment for 1 h prior to the addition of NGF indicated ... confocal microscopy. The projection of multiple sections is seen on the left for each panel to visualize the morphology of the cells. The middle row shows...

Ngày tải lên: 16/03/2014, 16:20

... modifiers in the domain of cooking tasks. Much of this is pertinent to an examination of extent, in par- tieular the relation of different modifiers to the as- peetual category of an event (according ... frequency in time units, as in every 5 minutes. Duration of an action - delimiting the interval Here, Karlin enumerates the following kinds of modifier...

Ngày tải lên: 17/03/2014, 08:20

... except in the characters' dialogs. Behind the superficial dl~erence in the use of tenses stands the more important one in the basic meaning of the grammatical category of tense. The standard ... Hartley and Phoebe had been sent by their mother to fix the tail v-~hve of the windmilL b. In the great expanse of the prairie where they lived,...

Ngày tải lên: 18/03/2014, 02:20

... was imported and processed even in the absence of exoge- nous ATP. The yield of pTic22 import in the absence of ATP varied between 20–50% of that in the presence of ATP. The import of pMGD1 was ... [ 35 S]pTic22, [ 35 S]pMGD1 and [ 35 S]pSSU were added to the column in binding buffer and incubated for 45 min. The flow through after incubation (Ft), the...

Ngày tải lên: 23/03/2014, 07:20

... proteins of the thylakoid lumen. In addition, there are certain proteins that possess a thylakoid membrane anchor but expose the bulk of the poly- peptide chain and the prosthetic group involved in electron ... absence of specific translocase inhibitors (Fig. 5). A striking feature in the targeting process of Rieske protein is the remarkably slow sorting of the...

Ngày tải lên: 30/03/2014, 15:20