Báo cáo khoa học: Mapping of the 45M1 epitope to the C-terminal cysteine-rich part of the human MUC5AC mucin potx

... features; the number is the total number of the clusters; the sym- bol “+” in the Data column means which portion of the Gigaword data is used to cluster words; the sym- bol “S” and “SS” in parentheses ... tagging accuracies relative to the length of the spans. We can see that with the increase of the number of words governed by the token, the difficulty...

Ngày tải lên: 23/03/2014, 14:20

... Cell migration in the presence or absence of the chemotactic factor was evaluated by estimating the distance (in lm) migrated by the leading-front of the cell, after the method of Zigmond and Hirsch ... with other mechanisms [12,13], and may act at several steps in the cascade. MAPK phosphoryla- tions have an impact on cascade processes in the cyto- plasm, the nucleus,...

Ngày tải lên: 07/03/2014, 16:20

... MUC5AC, with epitopes located in the MUC5AC C-terminal cysteine-rich part. Further localization of the epitopes of 45M1, 2-12 M1 and 166M1 In order to further locate the epitopes of the hitherto unmapped ... in the N-terminal region of the C-terminal cysteine-rich part of MUC5AC, pre- sumably in the last CysD domain of the mucin. The epitope...

Ngày tải lên: 07/03/2014, 05:20

... surface of holo- TC. Western blot analysis of the CNBr cleavage fragments of TC allowed us to distribute the epitopes between two regions, which spanned either the second quarter of the TC sequence ... assigned to the second quarter of TC sequence, whereas epitope clusters 1 and 3 (group 2) were localized to the last quarter of the full-length sequence. Antig...

Ngày tải lên: 20/02/2014, 01:20

... Localization of the amino acids in the epitope for Mab-1. The structure shown is a ribbon representation of the coordinates of Stout et al. [40]. The side chains of the amino acids in the epitope and ... measurement of the increase in absorbance at 405 nm. The specific inhibitory activity of PAI-1 was calculated from the amount of PAI-1 that had to be ad...

Ngày tải lên: 20/02/2014, 11:20

... isoform of the prion protein in brains of individuals with these diseases. This abnormal form of the protein (PrP Sc ) is also suggested to be the infectious agent in the disease on the basis of infection ... PrP c have also been shown to influence the level of the activity of the protein, as protein with the sequence of the mouse ÔbÕ allele is more Corres...

Ngày tải lên: 21/02/2014, 00:20

... P2, P4 and P9–13 lowered the k rel values by c. 50%. The replacement of 3mm Mg 2+ with 3 mm Mn 2+ resulted in a restoration of the activity to the level of the k Mn obs of the unmodified enzyme (Table ... °C. c k Mg rel ¼ the ratio of the k obs values of modified deoxyribozyme to unmodified deoxyribozyme, in the presence of Mg 2+ . e k Mn rel ¼ the ratio of...

Ngày tải lên: 07/03/2014, 10:20

... We therefore mutated Asp36 and Glu39 of CP12 into Ala or Lys. To test whether the interactions of these mutants with the other partner, GAPDH were im- paired, we tried to reconstitute in vitro the ... of the C-terminus of CP12 to thioredoxin in the GAPDH ⁄ CP12 complex is specifically due to the inter- action of CP12 with GAPDH. Impact of cysteine residues of...

Ngày tải lên: 16/03/2014, 13:20

... by introducing the 3-carboxybenzyl group to the P1¢ side chain of the inhibitor together with the sulfhydryl zinc-binding moiety [32]. To analyze the binding mode of the sub- strate ⁄ inhibitor to the active ... the binding of the glutarate part of the inhibitor in the S1¢ pocket contributes to the inhibition effect of the specific inhibitor 2-PMPA...

Ngày tải lên: 23/03/2014, 07:20

... from the product-binding pocket of the CM site to the substrate-binding pocket of the PDH site, then the weak CM activity of the CM fragments might be due to slow product release or trapping of ... into individual domains of the T-protein. A further goal of the study was to ascertain whether feedback inhibition by Tyr might also involve a discrete region of...

Ngày tải lên: 31/03/2014, 07:20