Báo cáo khoa học: Protein folding intermediates of invasin protein IbeA from Escherichia coli pdf
... al. Protein folding intermediates FEBS Journal 275 (2008) 458–469 ª 2007 The Authors Journal compilation ª 2007 FEBS 469 Protein folding intermediates of invasin protein IbeA from Escherichia coli Damodara ... Gdm-HCl-induced unfolding; Escherichia coli; molten globule; protein unfolding intermediates of IbeA Correspondence K. S. Kim, Division of Pediatr...
Ngày tải lên: 07/03/2014, 05:20
... of the Sir2 family of NAD + -dependent histone ⁄ protein deacetyl- ases. Biochemistry 43, 9877–9887. 51 Grozinger CM, Chao ED, Blackwell HE, Moazed D & Schreiber SL (2001) Identification of ... Identification of a small molecule inhi- bitor of Sir2p. Proc Natl Acad Sci USA 98, 15113– 15118. 53 Posakony J, Hirao M, Stevens S, Simon JA & Bedalov A (2004) Inhibitors of Sir2:...
Ngày tải lên: 20/02/2014, 01:20
... and refolding. Tot al activity, 1 U ¼ 1 lmol of s ubstrate hydrolysed per min. Steps Protein amount (mg) Total activity (U) Amount of active protein (mg) Specific activity (UÆmg )1 ) % of refolding Inclusion ... performed in 30 mL of 2.5 m M of Tris/HCl buffer, pH 7.0 and 0.1 M NaCl. Release of fatty acid was titrated with 0.1 M NaOH. Enzymatic activity was expressed in units (...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: The enzymatic activity of SR protein kinases 1 and 1a is negatively affected by interaction with scaffold attachment factors B1 and 2 pot
... significance of enzyme–substrate contacts for catalysis. This study highlights the importance of the interaction of a phosphoserine of the RS domain of the substrate with the catalytic region of the ... known to be protein protein and protein RNA interaction surfaces and the phos- phorylation of serines affects those interactions as well as interactions between RS domain-conta...
Ngày tải lên: 30/03/2014, 01:20
Tài liệu Báo cáo khoa học: Post-translational cleavage of recombinantly expressed nitrilase from Rhodococcus rhodochrous J1 yields a stable, active helical form ppt
... number of degrees of freedom. The twofold axis of the dimer model must coincide with the appropriate twofold axis of the stain envelope. Thus, the two possible degrees of freedom (apart from the ... stereo- chemical quality of protein structures. J Appl Crystal- logr 26, 283–291. 31 Sippl M (1993) Recognition of errors in three-dimen- sional structures of proteins. Protein...
Ngày tải lên: 19/02/2014, 00:20
Tài liệu Báo cáo khoa học: "Sense-based Interpretation of Logical Metonymy Using a Statistical Method" pdf
... This means that sense 3 of watch, sense 3 of view and sense 7 of see would be correct interpretations of the metonymic expression. The obtained number of synsets ranges from 14 (try shampoo) to ... covers 80% of their lists (our system does not find some of the low- probability range verbs of Lapata and Lascarides). We compared the rankings of the two implemen- tations in t...
Ngày tải lên: 20/02/2014, 09:20
Báo cáo khoa học: Molecular identification of monomeric aspartate racemase from Bifidobacterium bifidum pptx
... unit of the enzyme was defined as the amount of enzyme that catalyzes the formation of 1 lmol of D -aspartate from L -aspartate p er hour. Protein concentrations were determined using a protein assay ... molecular marker proteins; lane 1, the cell extract of E. coli JM109 (10 lg); lane 2, t he cell extract of the E. coli JM109/pBASPR c lone (10 lg); and lane 3, the enzyme...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Macrocypins, a family of cysteine protease inhibitors from the basidiomycete Macrolepiota procera pot
... con- tents of proline, of around 9.2% (the average overall protein content is 4.7 %), of tryptophan, of 3.6% (average 1.1 %) and of tyrosine, of 6.0–6.6% (average 2.9%), and low leucine contents of around ... sequence for the lower-molecular-mass protein could not be determined, possibly because of a blocked N terminus. The pres- ence of a protein of similar size to...
Ngày tải lên: 16/03/2014, 02:20
Báo cáo khoa học: "A Comparative Study of Reinforcement Learning Techniques on Dialogue Management" pdf
... Proceedings of the 12th Confer- ence of the European Chapter of the ACL (EACL 2009), pp 683–691. Ross, S., Pineau, J., Paquet, S., Chaib-draa, B., 2008, Online planning algorithms for POMDPs, Journal of ... Policy Q Learning No Off Value Q(λ) No Off Value Actor Critic - QV No On Policy IAC No On Policy NAC No On Policy DynaSARSA(λ) Yes On Value DynaQ Yes Off Value DynaQ(λ) Yes Off Val...
Ngày tải lên: 17/03/2014, 22:20
Báo cáo khoa học: X-ray structure of glucose/galactose receptor from Salmonella typhimurium in complex with the physiological ligand, (2R)-glyceryl-b-D-galactopyranoside pdf
... dimer of the Escherichia coli galactose-binding protein. Eur J Biochem 69, 545–550. 24 Vyas NK, Vyas MN & Quiocho FA (1988) Sugar and signal-transducer binding sites of the Escherichia coli galactose ... isolated from a mutant negative in the b-meth- ylgalactoside transport system of Escherichia coli. J Biol Chem 247, 5414–5424. 2 Hazelbauer GL & Adler J (1971) Rol...
Ngày tải lên: 23/03/2014, 04:21