Báo cáo khoa học: Kinetically controlled refolding of a heat

Báo cáo khoa học: Kinetically controlled refolding of a heat-denatured hyperthermostable protein pot

... Denaturation on heating (a) at a heating rate of 0.1 °CÆh )1 and the exothermic peak (b) observed on cooling with the same scanning rate. Fig. 3. LamA refolding as a function of time: (a) heat ... unfolding ⁄ refolding process of LamA was moni- tored using calorimetry. Analysis of the thermograms Fig. 4. Enzymatic activity of native and heat-treated LamA at differ- ent stor...

Ngày tải lên : 07/03/2014, 05:20

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Báo cáo khoa học: Chaperone-assisted refolding of Escherichia coli maltodextrin glucosidase doc

... concentration of urea was measured and plotted against the concentration of urea. Percent- age of residual activity was expressed relative to activity obtained from same amount of native protein. (B) MalZ ... 299, 713–716. 23 da Costa MS, Santos H & Galinski EA (1998) An over- view of the role and diversity of compatible solutes in Bacteria and Archaea. Adv Biochem Eng Biotec...

Ngày tải lên : 16/03/2014, 06:20

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Tài liệu Báo cáo khoa học: C-Terminal extension of a plant cysteine protease modulates proteolytic activity through a partial inhibitory mechanism doc

... CT ex 114 aa 208 aa 24 aa BamH 34 aa Start Stop Xho1 N -Pro Protease domain Protease domain CT - ex 380 aa 1 II NP 114 aa 208 aa BamH1 34 aa Start Stop Xho1 SS SS G SSS S N-Pro 356 aa III MSTLFII S ILLFLAS F SYAMDI S TIEYKYDKSS AWRTDEEVKEIYELWLAKHDKVY SG LVEYEKRFEIFKDNLKFIDEH NSENHTYKMGLTPYTDLTNEEFQAIYLGTRSDTIHRLKRTINISERYAYEAGDNLPEQIDWRKKGAVTPVKNQGKCG SCWAFSTVSTVESINQIRTGNLISLSEQQLVDCNKKNHG...

Ngày tải lên : 14/02/2014, 14:20

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Tài liệu Báo cáo khoa học: Intrinsic GTPase activity of a bacterial twin-arginine translocation proofreading chaperone induced by domain swapping ppt

... TorD GTPase activity can be isolated by Cibacron Blue affinity chromatography. (A) Unusual behaviour of TorD on CibacronÔ Blue affinity media. A sample of 0.5 m M metal affinity chromatography-purified ... malachite green GTPase assay. The fraction at 33 mL contained no detectable protein and was assayed as an internal control to establish that incubation with the column buffers alone...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc

... structure of micelle-associated VpUcyt was calculated on the basis of the ﬁnal set of proton–proton dis- tance constraints using cyana, a software program that combines simulated annealing with ... introduction of paramagnetic probes into the interior of the micelle or the aqueous buffer. Qualitative analysis of secondary chemical shift and paramagnetic relaxation enhancement...

Ngày tải lên : 18/02/2014, 06:20

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Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the ﬁlamentous fungus Trichoderma reesei ppt

... rounds and tested for tyrosinase activity with a plate assay. In the assay plates, Trichoderma minimal med- ium [19] with 2% lactose as a carbon source, 1% potassium phthalate as a buffering agent ... compounds of phenolic compounds, etc. and use thereof, European patent 919628. 36 Yamada Y (1983) Production of heat-resistant polyphenol oxidase, Japanese patent 61115488. 37 Yamada...

Ngày tải lên : 19/02/2014, 06:20

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Tài liệu Báo cáo khoa học: Structure-activity relationships of a-conotoxins targeting neuronal nicotinic acetylcholine receptors ppt

... University of Queensland, Brisbane, QLD, Australia a- Conotoxins that target the neuronal nicotinic acetylcholine receptor have a range of potential therapeutic applica- tions and are valuable probes ... mutational analysis has been carried out on ImI, PnIA, PnIB and to a lesser extent on GID and MII. Analysis of ImI has revealed that Asp5-Pro6-Arg7 and Trp10 are important for bi...

Ngày tải lên : 19/02/2014, 12:20

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Tài liệu Báo cáo khoa học: Novel aggregate formation of a frame-shift mutant protein of tissue-nonspeciﬁc alkaline phosphatase is ascribed to three cysteine residues in the C-terminal extension pdf

... Michigami T, Yamamoto T, Yasui N, Satomura K, Yamagata M, Shima M, Nakajima S, Mushiake S, Okada S & Ozono K (1998) Analysis of localization of mutated tissue-nonspeciﬁc alkaline phosphatase proteins associated ... fraction). BSA (b, 68 kDa), alcohol dehydrogenase (a, 141 kDa) and catalase (250 kDa) were loaded on to a separate gradient as mole- cular mass markers. K. Komaru et al....

Ngày tải lên : 19/02/2014, 17:20

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Tài liệu Báo cáo khoa học: Photochemical cross-linking of Escherichia coli Fpg protein to DNA duplexes containing pdf

... The yield of the complex was calculated as the ratio of shifted band radioactivity to the total radioactivity of the loaded sample. The apparent dissociation constants were deter- mined as described ... Equal mobilities of the radioactive and the protein- containing bands indicated covalent attachment of DNA to the enzyme. The yield of the photochemical cross-linking reaction was...

Ngày tải lên : 20/02/2014, 11:20

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Tài liệu Báo cáo khoa học: Site-directed mutagenesis of a loop at the active site of E1 (a2b2) of the pyruvate dehydrogenase complex A possible common sequence motif docx

... DCPIP assay, the E1aY28 1A and E1aR28 2A mutants displayed a catalytic activity about twice that of wild-type E1. The single mutants E1aR26 7A and E1aD27 6A and the multiple mutants E1aY28 1A/ R28 2A/ S28 3A ... 16-fold molarexcessofdi-domainoverE1.Lane1:E1wild-type;lane2:E1 wild-type + di-domain; lane 3: E1aF26 6A mutant; lane 4: E1aF26 6A mutant + di-domain; lane 5: E1aR26 7A mu...

Ngày tải lên : 20/02/2014, 23:20

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